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    • 92. 发明授权
    • Assays and other reactions involving droplets
    • 测定和其他涉及液滴的反应
    • US09029085B2
    • 2015-05-12
    • US12529926
    • 2008-03-07
    • Jeremy AgrestiLiang-Yin ChuDavid A. WeitzJin-Woong KimAmy RowatMorten SommerGautam DantasGeorge Church
    • Jeremy AgrestiLiang-Yin ChuDavid A. WeitzJin-Woong KimAmy RowatMorten SommerGautam DantasGeorge Church
    • C12Q1/68C12P19/34B01J13/00B01F3/08B01F13/00
    • C12P19/34B01F3/0807B01F3/0811B01F13/0062B01F13/0071B01F2215/0037B01J13/0052B01J13/0065B01L3/502784C12Q1/6834C12Q1/6848C12Q1/686G01N15/1404G01N2015/1006
    • The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
    • 本发明通常涉及液滴和/或乳液,例如多重乳液。 在一些情况下,液滴和/或乳液可用于测定中,并且在某些实施方案中,液滴或乳液可被硬化以形成凝胶。 在一些方面,可以使用凝胶进行异质测定。 例如,液滴可以被硬化以形成凝胶,其中液滴包含细胞,DNA或其它合适的物质。 凝胶可以暴露于反应物,并且反应物可以以某种方式与凝胶和/或与细胞,DNA等相互作用。 例如,反应物可以扩散通过凝胶,或者硬化的颗粒可以液化以形成液体状态,允许反应物与细胞相互作用。 作为具体实例,包含在凝胶颗粒内的DNA可以进行PCR(聚合酶链式反应)扩增,例如通过使用能够在形成凝胶时结合凝胶的PCR引物。 当使用PCR扩增DNA时,一些DNA将通过PCR引物与凝胶结合。 PCR反应后,未结合的DNA可以从凝胶中去除,例如通过扩散或洗涤。 因此,可以在本发明的一个实施方案中形成具有结合DNA的凝胶颗粒。
    • 93. 发明授权
    • Apparatus and method for restoring multi-channel audio signal using HE-AAC decoder and MPEG surround decoder
    • 使用HE-AAC解码器和MPEG环绕解码器恢复多声道音频信号的装置和方法
    • US08611547B2
    • 2013-12-17
    • US12307289
    • 2007-07-04
    • Jeong-Il SeoSeung-Kwon BeackIn-Seon JangDae-Young JangJin-Woo HongJin-Woong Kim
    • Jeong-Il SeoSeung-Kwon BeackIn-Seon JangDae-Young JangJin-Woo HongJin-Woong Kim
    • H04R5/00
    • G10L19/008G10L19/02G10L19/18
    • Provided is a method for controlling synchronizing downmix signals and MPEG surround side information signals by controlling a delay according to the kind of downmix audio signals in an MPEG surround decoder. When multi-channel audio signals are restored using an HE-AAC decoder and a low-power MPEG surround decoder and complex QMF signals outputted from the HE-AAC decoder are used as downmix signals, a delay unit compensates for a delay caused in a real-to-complex converter. Anther delay unit delays spatial parameters to compensate for a delay caused in QMF and Nyquist banks when time-domain downmix signals are used. Also, when multi-channel audio signals are restored using an HE-AAC decoder and a high-quality MPEG surround decoder and complex QMF signals outputted from the HE-AAC decoder are used as downmix signals, a delay unit compensates for a delay caused in a real-to-complex converter.
    • 提供了一种通过根据MPEG环绕解码器中的下混音频信号的种类来控制延迟来控制同步下混信号和MPEG环绕侧信息信号的方法。 当使用HE-AAC解码器和低功率MPEG环绕解码器来恢复多声道音频信号时,使用从HE-AAC解码器输出的复杂QMF信号作为下混合信号,延迟单元补偿实际产生的延迟 复合转换器。 花时延单元延迟空间参数以补偿在使用时域降混信号时在QMF和奈奎斯特银行中引起的延迟。 此外,当使用HE-AAC解码器和高质量MPEG环绕解码器恢复多声道音频信号并且将从HE-AAC解码器输出的复杂QMF信号用作下混合信号时,延迟单元补偿由 一个真实到复杂的转换器。
    • 98. 发明申请
    • ASSAYS AND OTHER REACTIONS INVOLVING DROPLETS
    • 测量和其他涉及倾销的反应
    • US20100136544A1
    • 2010-06-03
    • US12529926
    • 2008-03-07
    • Jeremy AgrestiLiang-Yin ChuDavid A. WeitzJin-Woong KimAmy RowatMorten SommerGautam DantasGeorge Church
    • Jeremy AgrestiLiang-Yin ChuDavid A. WeitzJin-Woong KimAmy RowatMorten SommerGautam DantasGeorge Church
    • C12Q1/68C12Q1/04
    • C12P19/34B01F3/0807B01F3/0811B01F13/0062B01F13/0071B01F2215/0037B01J13/0052B01J13/0065B01L3/502784C12Q1/6834C12Q1/6848C12Q1/686G01N15/1404G01N2015/1006
    • The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
    • 本发明通常涉及液滴和/或乳液,例如多重乳液。 在一些情况下,液滴和/或乳液可用于测定中,并且在某些实施方案中,液滴或乳液可被硬化以形成凝胶。 在一些方面,可以使用凝胶进行异质测定。 例如,液滴可以被硬化以形成凝胶,其中液滴包含细胞,DNA或其它合适的物质。 凝胶可以暴露于反应物,并且反应物可以以某种方式与凝胶和/或与细胞,DNA等相互作用。 例如,反应物可以扩散通过凝胶,或者硬化的颗粒可以液化以形成液体状态,允许反应物与细胞相互作用。 作为具体实例,包含在凝胶颗粒内的DNA可以进行PCR(聚合酶链式反应)扩增,例如通过使用能够在形成凝胶时结合凝胶的PCR引物。 当使用PCR扩增DNA时,一些DNA将通过PCR引物与凝胶结合。 PCR反应后,未结合的DNA可以从凝胶中去除,例如通过扩散或洗涤。 因此,可以在本发明的一个实施方案中形成具有结合DNA的凝胶颗粒。