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91. 发明专利

AU5136200A Chloramphenicol biosynthetic pathway and gene cluster characterization 未知
公开(公告)号：AU5136200A
公开(公告)日：2000-12-05
申请号：AU5136200
申请日：2000-05-15
申请人： DIVERSA CORP
发明人： PARADKAR ASHISH , SHORT JAY M , MATHUR ERIC J , GREEN BRIAN D
IPC分类号： C12N15/10 , C12N15/52 , C12P13/02 , C12Q1/68
摘要： The present invention provides a chloramphenicol gene cluster and methods of use thereof. Such gene clusters are useful for production of chloramphenicol.

92. 发明专利

CA2374532A1 RECOMBINANT BACTERIAL PHYTASES AND USES THEREOF 未知
公开(公告)号：CA2374532A1
公开(公告)日：2000-11-30
申请号：CA2374532
申请日：2000-05-25
申请人： DIVERSA CORP
发明人： SHORT JAY M , KRETZ KEITH A
IPC分类号： A01H5/00 , A01K67/027 , A21D8/04 , A23K1/16 , A23K1/165 , A23L5/20 , A23L7/10 , A23L11/00 , A23L11/30 , A23L29/00 , A23L33/00 , A61K36/18 , A61K38/00 , A61K38/46 , A61P1/14 , C12M1/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/02 , C12N5/10 , C12N9/16 , C12N15/09 , C12N15/55 , C12Q1/68 , A23L1/015 , A61K38/43
摘要： A purified recombinant phytase enzyme derived from Escherichia coliB. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity. The enzyme can be produced from native or recombinant host cells a nd can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve th e feeding value of phytate rich ingredients.

93. 发明专利

AU3879300A End selection in directed evolution 未知
公开(公告)号：AU3879300A
公开(公告)日：2000-09-28
申请号：AU3879300
申请日：2000-03-09
申请人： DIVERSA CORP
发明人： SHORT JAY M , FREY GERHARD JOHANN
IPC分类号： C12N9/14 , C12N15/10 , C40B40/02 , C40B50/06 , C12Q1/68
摘要： This invention provides methods of obtaining novel polynucleotides and encoded polypeptides by the use of non-stochastic methods of directed evolution (DirectEvolution™). A particular advantage of end-selection-based methods is the ability to recover full-length polynucleotides from a library of progeny molecules generated by mutagenesis methods. These methods include non-stochastic polynucleotide site-saturation mutagenesis (Gene Site Saturation Mutagenesis™) and non-stochastic polynucleotide reassembly (GeneReassembly™). This invention provides methods of obtaining novel enzymes that have optmized physical and/or biological properties. Through use of the claimed methods, genetic vaccines, enzymes, small molecules, and other desirable molecules can be evolved towards desirable properties. For example, vaccine vectors can be obtained that exhibit increased efficacy for use as genetic vaccines. Vectors obtained by using the methods can have, for example, enhanced antigen expression, increased uptake into a cell, increased stability in a cell, ability to tailor an immune response, and the like. Furthermore, this invention provides methods of obtaining a variety of novel biologically active molecules, in the fields of antibiotics, pharmacotherapeutics, and transgenic traits.

94. 发明专利

CA2330827A1 CONSTRUCTION AND USE OF CATALOGUED NUCLEIC ACID LIBRARIES THAT CONTAIN ADVANTAGEOUSLY ADJUSTED REPRESENTATIONS OF DEFINED COMPONENTS 未知
公开(公告)号：CA2330827A1
公开(公告)日：1999-12-09
申请号：CA2330827
申请日：1999-06-03
申请人： DIVERSA CORP
发明人： SHORT JAY M
IPC分类号： C12N15/09 , C07B61/00 , C07H21/00 , C12Q1/68 , C12N1/00 , C12N9/00 , C12N11/00 , C12Q1/00 , C12N15/10
摘要： A process for constructing a catalogued nucleic acid library in which the proportional representation of the constituents is adjusted to advantage through the use of disclosed technologies for positive and negative selection. The resultant benefit is that significantly fewer library constituents will need to be screened in order to identify a potentially desired constituent. Moreover, library constituents that previously would have been essentially "lost" are now recoverable. Preferred embodiments of this invention include the cataloguing, normalization, and enrichment of library constituents. By way of example, but not limitation, this technology is serviceable for constructing a library that contains an adequate representation of desirable constituents that (1) are initially found in low-copy numbers within a sample source or (2) originate from an organism that is problematic to culture. Applicable uses of this invention include any library-screening endeavor previously hindered by logistical impediments. By expanding previous logistical frontiers this invention allows for a novel generation of previously unattainable molecules - particularly molecules that are "unclonable" from conventional, unadjusted libraries - to now be detected, cloned, manipulated, expressed, studied, and used. By disclosing the construction and screening of high yielding nucleic acid libraries from mixed and uncultivated organisms, the instant technology eclipses former boundaries in the area of biological discovery and enables the full breadth of biological diversity to be accessed in the search for previously undiscovered genes and gene products. The benefits of the present invention are seen to extend to areas of diagnosis, medicine, agriculture, manufacturing, and academia.

95. 发明专利

CA2301953A1 COATED SURFACES FOR SELECTIVE ENRICHMENT OF MICROBIAL POPULATIONS 未知
公开(公告)号：CA2301953A1
公开(公告)日：1999-03-04
申请号：CA2301953
申请日：1998-08-25
申请人： DIVERSA CORP
发明人： SHORT JAY M
IPC分类号： C12M1/40 , C12M1/16 , C12M1/18 , C12M1/26 , C12Q1/24
摘要： The present invention relates to a method for collecting and concentrating microbes from complex or dilute microbial populations in aqueous or terrestrial environments. The present invention also relates to a method for collecting and concentrating microbes using monomers of polymeric substances conjugated to surface materials.

96. 发明专利

CA2259628A1 METHOD OF DNA SHUFFLING WITH POLYNUCLEOTIDES PRODUCED BY BLOCKING OR INTERRUPTING A SYNTHESIS OR AMPLIFICATION PROCESS 未知
公开(公告)号：CA2259628A1
公开(公告)日：1998-01-15
申请号：CA2259628
申请日：1997-07-09
申请人： DIVERSA CORP
发明人： SHORT JAY M
IPC分类号： C12N15/09 , C07K2/00 , C12N15/10 , C12P21/02 , C12Q1/68 , C12P19/34
摘要： Disclosed is a process of performing "Sexual" PCR which includes generating random polynucleotides by interrupting or blocking a synthesis or amplification process to show or halt synthesis or amplification of at least one polynucleotide, optionally amplifying the polynucleotides, and reannealing the polynucleotides to produce random mutant polynucleotides. Also provided are vector and expression vehicles including such mutant polynucleotides, polypeptides expressed by the mutant polynucleotides and a method for producing random mutant polypeptides.

97. 发明专利

BRPI0111124B1 polipeptídeo sintético ou recombinante com atividade fitase, polinucleotídeo codificando os mesmo, sistema de expressão e vetor compreendendo o referido polinucleotídeo, célula hospedeira compreendendo os mesmos, alimento compreendendo os mesmos, bem como métodos de aperfeiçoamento de valor nutricional e produção de um alimento 未知
公开(公告)号：BRPI0111124B1
公开(公告)日：2016-03-08
申请号：BR0111124
申请日：2001-05-24
申请人： DIVERSA CORP , VERENIUM CORP
发明人： O'DONOGHUE EILLEN , MATHUR ERIC J , GARRETT JAMES B , SHORT JAY M , KRETZ KEITH A , GRAY KEVIN A , BARTON NELSON ROBERT
IPC分类号： A01H5/00 , A01K67/027 , A21D8/04 , A23J3/14 , A23K1/14 , A23K1/165 , A23L1/30 , A23L5/20 , A23L7/104 , A23L11/00 , A23L11/30 , A23L29/00 , A61K36/48 , A61K38/00 , A61K38/46 , A61P1/14 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/02 , C12N5/10 , C12N9/16 , C12N15/09 , C12N15/14 , C12N15/29 , C12N15/55 , C12N15/63 , C12N15/79 , C12N15/82 , C12R1/84 , G06F17/30
摘要： "fitases bacterianas recombinantes e os usos das mesmas". é fornecida uma enzima fitase purificada e modificada da fitase appa de escherichia coli k12. a enzima possui atividade da fitase e aumentou a tolerância térmica quando comparada com a enzima do tipo selvagem. em adição, a enzima aumentou a estabilidade a proteases em ph baixo. a glicosilação da fitase modificada forneceu uma enzima melhorada adicional que possui tolerância térmica e estabilidade a proteases aumentadas. a enzima pode ser produzida partindo de células hospedeiras nativas ou recombinantes e pode ser utilizada para auxiliar na digestão da fitase quando desejado. em particular, a fitase da presente invenção pode ser utilizada em produtos alimentícios para aumentar o valor de alimentação de ingredientes ricos em fitase.

98. 发明专利

CA2492661C NON-STOCHASTIC GENERATION OF GENETIC VACCINES AND ENZYMES 未知
公开(公告)号：CA2492661C
公开(公告)日：2014-11-18
申请号：CA2492661
申请日：2000-02-04
申请人： DIVERSA CORP
发明人： SHORT JAY M
IPC分类号： C12N15/10 , A61K38/02 , A61K39/00 , C07K16/00 , C12P19/34 , C12P21/00 , C12Q1/68
摘要： This invention provides methods of obtaining novel polynucleotides and encoded polypeptides by use of non-stochastic methods of directed evolution (DirectEvolution.TM.). These methods include non-stochastic polynucleotide site-saturation mutagenesis (Gene Site Saturation Mutagenesis.TM.) and non-stochastic polynucleotide reassembly (GeneReassembly.TM.). Through use of the claimed methods, genetic vaccines, enzymes, and other desirable molecules can be evolved towards desirable properties. For example, vaccine vectors can be obtained that exhibit increased efficacy for use as genetic vaccines. Vectors obtained by using the methods can have, for example, enhanced antigen expression, increased uptake into a cell, increased stability in a cell, ability to tailor an immune response, and the like. This invention provides methods of obtaining novel enzymes that have optimized physical and/or biological properties. Furthermore, this invention provides methods of obtaining a variety of novel biologically active molecules, in the fields of antibiotics, pharmacotherapeutics, and transgenic traits.

99. 发明专利

CA2486062C NITRILASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 未知
公开(公告)号：CA2486062C
公开(公告)日：2014-10-14
申请号：CA2486062
申请日：2003-05-15
申请人： DIVERSA CORP
发明人： DESANTIS GRACE , SHORT JAY M , BURK MARK , WONG KELVIN , FARWELL ROBERT , CHATMAN KELLY
IPC分类号： C12N15/60 , C07H21/00 , C12N1/21 , C12N9/78 , C12N9/88 , C12N11/00 , C12N15/55 , C12P7/02 , C12P7/42 , C12P7/56 , C12P13/00 , C12P13/04 , C12P41/00 , C12Q1/34 , C12Q1/527
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

100. 发明专利

AU2003225947A8 Amylases, nucleic acids encoding them and methods for making and using them 未知
公开(公告)号：AU2003225947A8
公开(公告)日：2010-02-11
申请号：AU2003225947
申请日：2003-03-24
申请人： DIVERSA CORP
发明人： MATHUR ERIC , CALLEN WALTER , KAZAOKA MARTIN , FREY GERHARD , SHORT JAY M , RICHARDSON TOBY , MILLER CARL A
IPC分类号： C12N1/20 , C07H21/04 , C07K1/00 , C07K14/00 , C07K17/00 , C12N1/21 , C12N9/00 , C12N9/10 , C12N9/28 , C12N15/00 , C12N15/09 , C12P19/14

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