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91. 发明申请

US20110159481A1 Solid-phase chelators and electronic biosensors 有权
标题翻译：固相螯合剂和电子生物传感器
公开(公告)号：US20110159481A1
公开(公告)日：2011-06-30
申请号：US12655459
申请日：2009-12-30
申请人： David J. Liu , Xing Su , Kai Wu
发明人： David J. Liu , Xing Su , Kai Wu
IPC分类号： C12Q1/68 , G01N33/50 , G01N27/26
CPC分类号： C12Q1/48 , C12Q1/6837 , C12Q1/6869 , G01N27/4145 , G01N2333/9125 , C12Q2565/607 , C12Q2565/507 , C12Q2565/301
摘要： Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and electronically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced. Additionally, devices and methods are provided for detecting biomolecules through the detection of pyrophosphate ions.
摘要翻译：介绍了核酸测序方法。通过化学扩增DNA合成产物和化学扩增产物的检测来完成测序。在本发明的实施方案中，提供了具有多个待测序的DNA分子的底物和能够螯合连接的焦磷酸根离子的多个分子，引入待测序的DNA分子，并且掺入下一个互补核苷酸，切除多次导致待测序的DNA分子局部周围的焦磷酸盐离子的积聚。焦磷酸根离子被底物连接的螯合剂捕获并电子检测以确定待测序的DNA分子中下一个互补核酸的同一性。另外，提供了通过检测焦磷酸根离子来检测生物分子的装置和方法。

92. 发明授权

US07714118B2 Polynucleotides encoding the fkbB gene of the FK-520 polyketide synthase gene cluster 有权
标题翻译：编码FK-520聚酮化合物基因簇的fkbB基因的多核苷酸
公开(公告)号：US07714118B2
公开(公告)日：2010-05-11
申请号：US10885305
申请日：2004-07-06
申请人： Christopher Reeves , Kai Wu
发明人： Christopher Reeves , Kai Wu
IPC分类号： C12P19/62 , C12P17/18 , C12P19/00 , C12P19/32 , C12N15/31 , C12N15/52 , C12N15/60 , C12N15/79
CPC分类号： C07D498/18 , C07K2319/00 , C12N9/1029 , C12N9/1205 , C12N15/52 , C12P17/188 , C12P19/32
摘要： Host cells comprising recombinant vectors encoding the FK-520 polyketide synthase and FK-520 modification enzymes can be used to produce the FK-520 polyketide. Recombinant DNA constructs comprising one or more FK-520 polyketide synthase domains, modules, open reading frames, and variants thereof can be used to produce recombinant polyketide synthases and a variety of different polyketides with application as pharmaceutical and veterinary products.
摘要翻译：包含编码FK-520聚酮化合物合成酶和FK-520修饰酶的重组载体的宿主细胞可用于产生FK-520聚酮化合物。包含一个或多个FK-520聚酮化合物结构域，模块，开放阅读框及其变体的重组DNA构建体可用于生产重组聚酮化合物合酶和各种不同的聚酮化合物，作为药物和兽用产品的应用。

93. 发明申请

US20090186378A1 Polynucleotides encoding the fkbB gene of the FK-520 polyketide synthase gene cluster 有权
标题翻译：编码FK-520聚酮化合物基因簇的fkbB基因的多核苷酸
公开(公告)号：US20090186378A1
公开(公告)日：2009-07-23
申请号：US10885305
申请日：2004-07-06
申请人： Christopher Reeves , Daniel Chu , Chaitan Khosla , Daniel Santi , Kai Wu
发明人： Christopher Reeves , Daniel Chu , Chaitan Khosla , Daniel Santi , Kai Wu
IPC分类号： C12P21/04 , C12N15/11 , C12P17/16 , C12N1/21 , C12N15/00
CPC分类号： C07D498/18 , C07K2319/00 , C12N9/1029 , C12N9/1205 , C12N15/52 , C12P17/188 , C12P19/32
摘要： Host cells comprising recombinant vectors encoding the FK-520 polyketide synthase and FK-520 modification enzymes can be used to produce the FK-520 polyketide. Recombinant DNA constructs comprising one or more FK-520 polyketide synthase domains, modules, open reading frames, and variants thereof can be used to produce recombinant polyketide synthases and a variety of different polyketides with application as pharmaceutical and veterinary products.
摘要翻译：包含编码FK-520聚酮化合物合成酶和FK-520修饰酶的重组载体的宿主细胞可用于产生FK-520聚酮化合物。包含一个或多个FK-520聚酮化合物结构域，模块，开放阅读框及其变体的重组DNA构建体可用于生产重组聚酮化合物合酶和各种不同的聚酮化合物，作为药物和兽用产品的应用。

94. 发明申请

US20080160630A1 Enzymatic signal generation and detection of binding complexes in stationary fluidic chip 有权
标题翻译：在固定流体芯片中的酶信号产生和结合复合物的检测
公开(公告)号：US20080160630A1
公开(公告)日：2008-07-03
申请号：US11647565
申请日：2006-12-29
申请人： David J. Liu , Kai Wu , Xing Su
发明人： David J. Liu , Kai Wu , Xing Su
IPC分类号： G01N21/00 , G01N1/00
CPC分类号： G01N21/658 , B01L3/50273 , B01L3/502761 , B01L2300/0654 , B01L2400/043 , B01L2400/086 , B82Y15/00 , B82Y30/00 , G01N21/255 , G01N21/6428 , G01N21/645 , G01N21/76 , G01N33/54333 , G01N33/587
摘要： An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.
摘要翻译：本发明的一个实施例涉及用于检测样品中的分析物的装置。该装置包括流体网络和集成电路部件。流体网络包括样品区，清洁区和检测区。流体网络包含磁性粒子和/或信号粒子。引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

95. 发明申请

US20070218478A1 Methods for fragmentation and analysis of nucleic acid 审中-公开
标题翻译：核酸分离和分析方法
公开(公告)号：US20070218478A1
公开(公告)日：2007-09-20
申请号：US11612454
申请日：2006-12-18
申请人： Qing Bai , Susana Salceda , Kai Wu , Thong Nguyen , Charles Miyada
发明人： Qing Bai , Susana Salceda , Kai Wu , Thong Nguyen , Charles Miyada
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6806 , C12Q2521/531 , C12Q2521/301 , C12Q2521/131
摘要： Methods for fragmenting and labeling DNA in a single reaction volume and incubation step using a uracil DNA glycosylase, an apurinic/apyrimidinic endonuclease, and a terminal transferase are disclosed. In a preferred embodiment the UDG, AP and TdT activities are first mixed together to form an enzyme mixture and then the enzyme mixture is mixed with the uracil containing DNA. The fragmentation and labeling reactions thus take place simultaneously as part of the same reaction. The methods may be used in a variety of applications where fragmenting and end-labeling single or double stranded DNA is desired.
摘要翻译：公开了在单个反应体积中分离和标记DNA的方法以及使用尿嘧啶DNA糖基化酶，无嘌呤/脱嘧啶核苷酸内切酶和末端转移酶的温育步骤。在优选的实施方案中，首先将UDG，AP和TdT活性混合在一起以形成酶混合物，然后将该酶混合物与含有尿嘧啶的DNA混合。因此，碎裂和标记反应同时作为相同反应的一部分进行。该方法可用于需要片段化和末端标记单链或双链DNA的多种应用。

96. 发明申请

US20070020654A1 Methods and kits for preparing nucleic acid samples 审中-公开
标题翻译：用于制备核酸样品的方法和试剂盒
公开(公告)号：US20070020654A1
公开(公告)日：2007-01-25
申请号：US11419459
申请日：2006-05-19
申请人： John Blume , Yanxiang Cao , Kyle Cole , Kai Wu , Charles Miyada
发明人： John Blume , Yanxiang Cao , Kyle Cole , Kai Wu , Charles Miyada
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6806 , C12Q2531/143 , C12Q2525/179 , C12Q2521/531 , C12Q2521/131
摘要： The present invention provides methods for preparing nucleic acid samples. The methods of the present invention are particularly amenable for preparing samples that substantially represent the whole transcripts. In some aspects the methods include a step of reducing the amount of ribosomal RNA in a total RNA sample prior to amplification. In preferred aspects single stranded sense strand cDNA is generated, labeled and hybridized to arrays of probes. The method is particularly suitable to use with microarray based expression analysis.
摘要翻译：本发明提供了制备核酸样品的方法。本发明的方法特别适于制备基本上代表整个转录物的样品。在一些方面，所述方法包括在扩增前减少总RNA样品中的核糖体RNA的量的步骤。在优选的方面，产生单链有义链cDNA，标记并与探针阵列杂交。该方法特别适用于基于微阵列的表达分析。

97. 发明申请

US20060235937A1 System and method for processing commands in a storage enclosure 有权
标题翻译：用于处理存储机柜中的命令的系统和方法
公开(公告)号：US20060235937A1
公开(公告)日：2006-10-19
申请号：US11108285
申请日：2005-04-18
申请人： Kai Wu , Farzad Khosrowpour
发明人： Kai Wu , Farzad Khosrowpour
IPC分类号： G06F15/167
CPC分类号： G06F15/16
摘要： A system and method for receiving and responding to issued commands in a storage enclosure is disclosed in which each storage enclosure processor of the storage enclosure is coupled to each expander of the storage enclosure. Each storage enclosure processor receives each interrupt and command issued by another expander of the storage enclosure. In the event of a failure of one of the storage enclosure processors, any interrupts or commands issued by the expander associated with the failed storage enclosure processor will be handled by the operational storage enclosure processor. In this configuration, the storage enclosure processor can also arbitrate or determine the storage enclosure processor that will handle each interrupt and any associated command.
摘要翻译：公开了一种用于接收和响应存储外壳中发布的命令的系统和方法，其中存储外壳的每个存储外壳处理器耦合到存储外壳的每个扩展器。每个存储机箱处理器接收由存储机柜的另一个扩展器发出的每个中断和命令。如果一个存储机箱处理器发生故障，则与故障存储机箱处理器相关联的扩展器发出的任何中断或命令将由操作存储机箱处理器处理。在这种配置中，存储机箱处理器还可以仲裁或确定将处理每个中断的存储机箱处理器和任何相关联的命令。

98. 发明申请

US20060194243A1 Methods for Identifying DNA Copy Number Changes 审中-公开
标题翻译：识别DNA拷贝数变化的方法
公开(公告)号：US20060194243A1
公开(公告)日：2006-08-31
申请号：US11381813
申请日：2006-05-05
申请人： Rajeswari Tadakamalla , Kai Wu
发明人： Rajeswari Tadakamalla , Kai Wu
IPC分类号： C12Q1/68 , G06F19/00
CPC分类号： C12Q1/6809 , G16B25/00 , Y02A90/26 , C12Q2565/501 , C12Q2531/119 , C12Q2521/301
摘要： Methods and computer software products for identifying changes in genomic DNA copy number are disclosed. Methods for identifying homozygous deletions and genetic amplifications are disclosed. Genomic DNA is amplified generically and amplified sample is hybridized to an expression array. The expression array comprises probes to regions of genes that are expressed. The probes are complementary to genomic sequences found in mRNAs. Signal intensity is correlated to copy number. The methods may be used to detect copy number changes in cancerous tissue compared to normal tissue. The methods may be used to diagnose cancer and other diseases associated with chromosomal anomalies.
摘要翻译：公开了用于鉴定基因组DNA拷贝数变化的方法和计算机软件产品。公开了鉴定纯合缺失和遗传扩增的方法。基因组DNA被放大一般，扩增的样品与表达数组杂交。表达阵列包括对表达的基因区域的探针。探针与mRNAs中发现的基因组序列互补。信号强度与拷贝数相关。与正常组织相比，该方法可用于检测癌组织中的拷贝数变化。该方法可用于诊断与染色体异常相关的癌症和其他疾病。

99. 发明申请

US20060174085A1 Storage enclosure and method for the automated configuration of a storage enclosure 审中-公开
标题翻译：用于自动配置存储机柜的存储机柜和方法
公开(公告)号：US20060174085A1
公开(公告)日：2006-08-03
申请号：US11046599
申请日：2005-01-28
申请人： Kai Wu , Farzad Khosrowpour
发明人： Kai Wu , Farzad Khosrowpour
IPC分类号： G06F12/00
CPC分类号： G11B33/12 , G06F3/0601 , G06F2003/0692
摘要： An architecture and a method for the automated configuration of a storage enclosure are disclosed. The disclosed storage enclosure includes an internal communications link between the storage controllers of the storage enclosure. The storage enclosure is operable to determine if the storage enclosure is a terminal storage enclosure or if the expansion ports of the storage enclosure are externally cabled. If the storage enclosure is the terminal storage enclosure, the internal communications link is enabled, providing a communications pathway between the two controllers that is internal to the storage enclosure.
摘要翻译：公开了用于存储外壳的自动配置的架构和方法。所公开的存储机箱包括存储机柜的存储控制器之间的内部通信链路。存储机箱可操作以确定存储机箱是终端存储机箱还是存储机柜的扩展端口是外部电缆连接。如果存储机柜是终端存储机柜，则启用内部通信链路，从而在存储机柜内部的两个控制器之间提供通信路径。

100. 发明申请

US20050123956A1 Methods for modifying DNA for microarray analysis 审中-公开
标题翻译：修改DNA用于微阵列分析的方法
公开(公告)号：US20050123956A1
公开(公告)日：2005-06-09
申请号：US10951983
申请日：2004-09-27
申请人： John Blume , Yanxiang Cao , Glenn McGall , Kyle Cole , Fredrick Christians , Kai Wu , Linda Hsie , Charles Miyada , Anthony Barone , Vivi Truong
发明人： John Blume , Yanxiang Cao , Glenn McGall , Kyle Cole , Fredrick Christians , Kai Wu , Linda Hsie , Charles Miyada , Anthony Barone , Vivi Truong
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/683 , C12Q2565/501 , C12Q2525/119 , C12Q2521/531
摘要： In one aspect of the invention, methods and compositions are provided for fragmenting nucleic acid samples. Fragmented nucleic acid samples may be used for hybridization with microarrays.
摘要翻译：在本发明的一个方面，提供了用于片段化核酸样品的方法和组合物。分离的核酸样品可用于与微阵列杂交。

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