专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

91. 发明专利

DE69636658D1 未知
公开(公告)号：DE69636658D1
公开(公告)日：2006-12-07
申请号：DE69636658
申请日：1996-12-05
申请人： STRATAGENE CALIFORNIA , CHILDRENS MEDICAL CENTER
发明人： BAUER C , WRIGHT A , BRAMAN C , GEHA S
IPC分类号： C12N15/09 , C12Q1/68 , C12N15/10 , C12P19/34
摘要： The invention provides improved method of introducing site-directed mutations into circular DNA molecules of interest by means of mutagenic primer pairs. The mutagenic primer pairs are also selected so as to be either completely complementary or partially complementary to each other, wherein the mutation site (or sites) is located within the region of complementarity. A mutagenic primer pair is annealed to opposite strands of a circular DNA molecule containing the DNA sequence to be mutagenized. After annealing, first and second mutagenized DNA strands , each incorporating a member of the mutagenic oligonucleotide primer pair is synthesized by a linear cyclic amplification reaction. After the linear cyclic amplification mediated synthesis step is completed, the reaction mixture is treated with a section enzyme that digests the parental template strands. After the digesting step, a double-stranded circular DNA intermediate is formed. The double-stranded circular DNA intermediates are transformed in suitable competent host cells and closed circular double-stranded DNA corresponding to the parental template molecules, but containing the desired mutation or mutations of interest, may be conveniently recovered from the transformed cells. The invention also provides kits for site-directed mutagenesis in accordance with methods of the present invention.

92. 发明专利

DE60027040T2 未知
公开(公告)号：DE60027040T2
公开(公告)日：2006-11-23
申请号：DE60027040
申请日：2000-10-27
申请人： STRATAGENE CALIFORNIA
发明人： SORGE A , HOGREFE H , HANSEN J
IPC分类号： C12N15/09 , C12N15/54 , C12N9/10 , C12N9/12 , C12N15/70 , C12P19/34 , C12Q1/48 , C12Q1/68
摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3' to 5' exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.

93. 发明专利

CA2608151A1 SNAPBACK OLIGONUCLEOTIDE PROBE 未知
公开(公告)号：CA2608151A1
公开(公告)日：2006-11-16
申请号：CA2608151
申请日：2006-05-10
申请人： STRATAGENE CALIFORNIA
发明人： SORGE JOSEPH A
IPC分类号： C12Q1/68 , C12P19/34
摘要： The invention relates to compositions and methods for detection of specific nucleic acid sequences during an amplification reaction. The invention furth er relates to a kit format of said compositions for detection of nucleic acid sequences. The Snapback oligonucleotide probes of the invention comprise a target binding sequence, a hairpin forming sequence, and a reporter binding sequence. The action of a nucleic acid polymerase or a flap endonuclease cleaves off a "snapback segment" of the probe containing the hairpin forming sequence and reporter binding sequence, causing the probe to "snap back" and form a hairpin structure. The further action of a nucleic acid polymerase or a flap endonuclease cleaves off a label moiety from a reporter oligonucleotide hybridized to the reporter binding sequence, resulting in a detectable signa l.

94. 发明专利

AU2006244035A1 Snapback oligonucleotide probe 未知
公开(公告)号：AU2006244035A1
公开(公告)日：2006-11-16
申请号：AU2006244035
申请日：2006-05-10
申请人： STRATAGENE CALIFORNIA
发明人： SORGE JOSEPH A
IPC分类号： C12Q1/68 , C12P19/34
摘要： The invention relates to compositions and methods for detection of specific nucleic acid sequences during an amplification reaction. The invention further relates to a kit format of said compositions for detection of nucleic acid sequences. The Snapback oligonucleotide probes of the invention comprise a target binding sequence, a hairpin forming sequence, and a reporter binding sequence. The action of a nucleic acid polymerase or a flap endonuclease cleaves off a "snapback segment" of the probe containing the hairpin forming sequence and reporter binding sequence, causing the probe to "snap back" and form a hairpin structure. The further action of a nucleic acid polymerase or a flap endonuclease cleaves off a label moiety from a reporter oligonucleotide hybridized to the reporter binding sequence, resulting in a detectable signal.

95. 发明专利

CA2519309A1 DNA POLYMERASE FUSIONS AND USES THEREOF 未知
公开(公告)号：CA2519309A1
公开(公告)日：2004-10-14
申请号：CA2519309
申请日：2004-03-19
申请人： STRATAGENE CALIFORNIA
发明人： BORNS MICHAEL
IPC分类号： C12N20060101 , C12N9/00 , C12N9/12 , C12N15/10 , C12P19/34 , C12Q1/68
摘要： The present invention discloses methods of using DNA polymerase fusions at high pH in PCR, DNA sequencing and mutagenesis protocols.

96. 发明专利

AU2002360638A1 HIGH FIDELITY DNA POLYMERASE COMPOSITIONS AND USES THEREFOR 未知
公开(公告)号：AU2002360638A1
公开(公告)日：2003-07-30
申请号：AU2002360638
申请日：2002-12-17
申请人： STRATAGENE CALIFORNIA
发明人： SORGE JOSEPH A , HOGREFE HOLLY , BORNS MICHAEL
IPC分类号： C12N15/09 , C12N9/10 , C12N9/12 , C12Q1/68
摘要： The subject invention relates to compositions comprising an enzyme mixture which comprises a first enzyme and a second enzyme, where the first enzyme comprises a DNA polymerization activity and the second enzyme comprises an 3′-5′ exonuclease activity and a reduced DNA polymerization activity. The invention also relates to the above compositions in kit format and methods for high fidelity DNA synthesis using the subject compositions of the invention.

97. 发明申请

WO2008094902A3 COMPOSITIONS AND METHODS FOR THE DETECTION OF A NUCLEIC ACID USING A CLEAVAGE REACTION 审中-公开
标题翻译：使用CLEAVAGE反应检测核酸的组合物和方法
公开(公告)号：WO2008094902A3
公开(公告)日：2011-07-21
申请号：PCT/US2008052304
申请日：2008-01-29
申请人： STRATAGENE CALIFORNIA , SORGE JOSEPH A , FIRMIN ANDREW , HAPPE SCOTT
发明人： SORGE JOSEPH A , FIRMIN ANDREW , HAPPE SCOTT
IPC分类号： C12N15/11 , C12Q1/68 , G01N33/50
CPC分类号： C12Q1/6844 , C12Q1/6823 , C12Q2525/307 , C12Q2565/101 , C12Q2521/301 , C12Q2525/161
摘要： The invention relates to compositions and methods for generating a signal indicative of the presence of a target nucleic acid in a sample utilizing a primer-probe duplex.
摘要翻译：本发明涉及使用引物 - 探针双链体产生指示样品中靶核酸存在的信号的组合物和方法。

98. 发明申请

WO2007022045A3 MUTANT REVERSE TRANSCRIPTASE AND METHODS OF USE 审中-公开
标题翻译：突变体逆转录酶及其使用方法
公开(公告)号：WO2007022045A3
公开(公告)日：2009-05-22
申请号：PCT/US2006031567
申请日：2006-08-10
申请人： STRATAGENE CALIFORNIA , HOGREFE HOLLY , AREZI BAHRAM , XING WEIMEI
发明人： HOGREFE HOLLY , AREZI BAHRAM , XING WEIMEI
IPC分类号： C12N9/00 , C07H21/04 , C12N9/12 , C12P19/34 , C12Q1/68
CPC分类号： C12Y207/07049 , C12N9/1276
摘要： The invention relates to the generation and characterization of stable MMLV reverse transcriptase mutants. The invention also discloses methods of using table MMLV reverse transcriptase mutants.
摘要翻译：本发明涉及稳定的MMLV逆转录酶突变体的产生和表征。本发明还公开了使用表MMLV逆转录酶突变体的方法。

99. 发明申请

WO2009008854A1 METHODS FOR DETECTION OF A TARGET NUCLEIC ACID BY FORMING A CLEAVAGE STRUCTURE USING A REVERSE TRANSCRIPTASE 审中-公开
标题翻译：通过使用反向转录物形成CLEAVAGE结构来检测目标核酸的方法
公开(公告)号：WO2009008854A1
公开(公告)日：2009-01-15
申请号：PCT/US2007/014590
申请日：2007-06-22
申请人： STRATAGENE CALIFORNIA , SORGE, Joseph, A.
发明人： SORGE, Joseph, A.
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6823 , C12Q2525/301 , C12Q2525/161 , C12Q2521/307
摘要： The invention relates to and methods for generating a signal indicative of the presence of a target nucleic acid in a sample. The compositions and methods include a reverse transcriptase, a nuclease, an upstream primer and downstream probe.
摘要翻译：本发明涉及产生指示样品中目标核酸存在的信号的方法。组合物和方法包括逆转录酶，核酸酶，上游引物和下游探针。

100. 发明申请

WO2008054556A3 METHODS AND COMPOSITIONS FOR DETECTION OF A TARGET NUCLEIC ACID SEQUENCE 审中-公开
公开(公告)号：WO2008054556A3
公开(公告)日：2008-05-08
申请号：PCT/US2007/014588
申请日：2007-06-22
申请人： STRATAGENE CALIFORNIA , SORGE, Joseph, A.
发明人： SORGE, Joseph, A.
IPC分类号： C12Q1/68
摘要： The invention relates to compositions and methods for generating a signal indicative of the presence of a target nucleic acid in a sample, where the compositions and methods include an RNA polymerase, a FEN nuclease, and a probe.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式