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1. 发明授权

EP0254771B1 Detection of microbes in a sample 有权
标题翻译：检测样品中的微生物
公开(公告)号：EP0254771B1
公开(公告)日：1992-12-02
申请号：EP86117754.1
申请日：1986-12-19
申请人： Edberg, Stephen C.
发明人： Edberg, Stephen C.
IPC分类号： C12Q1/04
CPC分类号： C12Q1/10 , C12Q1/04 , C12Q1/34

2. 发明公开

EP0254771A2 Detection of microbes in a sample 有权
标题翻译： Nachweis von Mikroben in einer Probe。
公开(公告)号：EP0254771A2
公开(公告)日：1988-02-03
申请号：EP86117754.1
申请日：1986-12-19
申请人： Edberg, Stephen C.
发明人： Edberg, Stephen C.
IPC分类号： C12Q1/04
CPC分类号： C12Q1/10 , C12Q1/04 , C12Q1/34
摘要： The presence or absence of a predetermined target microbe in a sample is determined by adding a testing medium to the sample, or vice versa. The testing medium provides a selective growth medium for the target microbe and includes a specific nutrient which only the target microbe can metabolize. This specific nutrient is modified by attaching a sample-altering moiety thereto, thereby converting the nutrient to a nutrient-indicator. The sample-altering moiety is activated to alter the sample only if the specific nutrient is metabolized by the target microbe. The sample-altering moiety can be a material which changes the color of the sample (visible or non-visible) or changes an electrical characteristic of the sample, or alters some other detectable characteristic of the sample. The testing media does not have to be kept sterile, and the testing procedure does not have to be performed in a sterile environment.
摘要翻译：通过向样品中加入测试培养基来确定样品中预定目标微生物的存在或不存在，反之亦然。测试培养基为目标微生物提供选择性生长培养基，并且包括只有目标微生物可代谢的特定营养物质。通过将样品改变部分附接到其上来改变该特定营养物，从而将营养物转化为营养指示剂。只有当特定营养物质被目标微生物代谢时，样品改变部分被激活才能改变样品。样品改变部分可以是改变样品的颜色（可见或不可见）或改变样品的电特性或改变样品的一些其它可检测特征的材料。测试介质不必保持无菌状态，测试程序不必在无菌环境中进行。

3. 发明授权

EP0254771B2 Detection of microbes in a sample 有权
标题翻译：的样品中微生物的检测
公开(公告)号：EP0254771B2
公开(公告)日：2000-02-02
申请号：EP86117754.1
申请日：1986-12-19
申请人： Edberg, Stephen C.
发明人： Edberg, Stephen C.
IPC分类号： C12Q1/04
CPC分类号： C12Q1/10 , C12Q1/04 , C12Q1/34

4. 发明公开

EP0254771A3 Detection of microbes in a sample 有权
标题翻译：检测样品中的微生物
公开(公告)号：EP0254771A3
公开(公告)日：1988-08-24
申请号：EP86117754
申请日：1986-12-19
申请人： Edberg, Stephen C.
发明人： Edberg, Stephen C.
IPC分类号： C12Q01/04
CPC分类号： C12Q1/10 , C12Q1/04 , C12Q1/34
摘要： The presence or absence of a predetermined target microbe in a sample is determined by adding a testing medium to the sample, or vice versa. The testing medium provides a selective growth medium for the target microbe and includes a specific nutrient which only the target microbe can metabolize. This specific nutrient is modified by attaching a sample-altering moiety thereto, thereby converting the nutrient to a nutrient-indicator. The sample-altering moiety is activated to alter the sample only if the specific nutrient is metabolized by the target microbe. The sample-altering moiety can be a material which changes the color of the sample (visible or non-visible) or changes an electrical characteristic of the sample, or alters some other detectable characteristic of the sample. The testing media does not have to be kept sterile, and the testing procedure does not have to be performed in a sterile environment.

5. 发明授权

US5429933A Detection of first generation environmental sourced microbes in an environmentally-derived sample 失效
标题翻译：在环境衍生的样品中检测第一代环境来源的微生物
公开(公告)号：US5429933A
公开(公告)日：1995-07-04
申请号：US323064
申请日：1994-10-14
申请人： Stephen C. Edberg
发明人： Stephen C. Edberg
IPC分类号： C12Q1/04 , C12Q1/10 , C12Q1/34
CPC分类号： C12Q1/045 , C12Q1/04 , C12Q1/10 , C12Q1/34 , Y10S435/802 , Y10S435/968
摘要： The presence or absence of a predetermined target first generation environmental sourced microbe in an environmentally derived sample is determined by adding a testing medium to the sample, or vice versa. The testing medium provides a selective growth medium for the target microbe and includes a specific nutrient which only the target microbe can significantly metabolize and use for growth This specific nutrient is modified by attaching a sample-altering moiety thereto, thereby converting the nutrient to a nutrient-indicator. The sample-altering moiety is activated to alter the sample only if the specific nutrient is metabolized by the target microbe. The sample-altering moiety can be a material which changes the color of the sample (visable or non-visable) or changes an electrical characteristic of the sample, or alters some other detectable characteristic of the sample. The testing media does not have to be kept sterile, (but, obviously must be free of viable target microbes) and the test ire procedure does not have to be performed in a sterile environment. The medium also includes an accelerant which hastens the advancement of the target microbes to the log phase of growth during the testing procedure.
摘要翻译：在环境衍生的样品中存在或不存在预定的目标第一代环境来源的微生物通过向样品中加入测试培养基来确定，反之亦然。测试培养基为目标微生物提供选择性生长培养基，并且包括只有目标微生物可以显着代谢并用于生长的特定营养物。通过将样品改变部分附加到其上来修饰该特定营养物，从而将营养物转化为营养物 -指示符。只有当特定营养物质被目标微生物代谢时，样品更换部分被激活才能改变样品。样品改变部分可以是改变样品的颜色（可见或不可见）或改变样品的电特性或改变样品的一些其它可检测特征的材料。测试介质不必保持无菌（但显然必须没有可行的目标微生物），并且测试过程不必在无菌环境中进行。该培养基还包括促进剂，其加速了目标微生物在测试过程中生长的对数阶段。

6. 发明授权

US4925789A Method and medium for use in detecting target microbes in situ in a specimen sample of a possibly contaminated material 失效
标题翻译：用于在可能被污染的材料的样本样品中原位检测目标微生物的方法和介质
公开(公告)号：US4925789A
公开(公告)日：1990-05-15
申请号：US880305
申请日：1986-06-30
申请人： Stephen C. Edberg
发明人： Stephen C. Edberg
IPC分类号： C12N1/20 , C12Q1/04 , C12Q1/10 , C12Q1/34 , C12Q1/37 , C12R1/19
CPC分类号： C12Q1/10 , C12Q1/04 , C12Q1/34
摘要： The presence or absence of a predetermined target microbe in a sample is determined by adding a testing medium to the sample, or vice versa. The testing medium provides a selective growth medium for the target microbe and includes a specific nutrient which only the target microbe can metabolize. This specific nutrient is modified by attaching a sample-altering moiety thereto, thereby converting the nutrient to a nutrient-indicator. The sample-altering moiety is activated to alter the sample only if the specific nutrient is metabolized by the target microbe. The sample-altering moiety can be a material which changes the color of the sample (visible or non-visible) or changes an electrical characteristic of the sample, or alters some other detectable characteristic of the sample. The testing media does not have to be kept sterile, and the testing procedure does not have to be performed in a sterile environment.
摘要翻译：通过向样品中加入测试培养基来确定样品中预定目标微生物的存在或不存在，反之亦然。测试介质为目标微生物提供选择性生长培养基，并且包括只有目标微生物可以代谢的特定营养物质。通过将样品改变部分附着到其上来修饰该特定营养物，从而将营养物转化为营养指示剂。只有当特定营养物质被目标微生物代谢时，样品更换部分被激活才能改变样品。样品改变部分可以是改变样品的颜色（可见或不可见）或改变样品的电特性或改变样品的一些其它可检测特征的材料。测试介质不必保持无菌状态，测试程序不必在无菌环境中进行。

7. 发明授权

US5780259A Medium and method for detecting a target microbe 失效
标题翻译：培养基和检测目标微生物的方法
公开(公告)号：US5780259A
公开(公告)日：1998-07-14
申请号：US465010
申请日：1995-06-05
申请人： Stephen C. Edberg
发明人： Stephen C. Edberg
IPC分类号： C12Q1/04 , C12Q1/10 , C12Q1/34
CPC分类号： C12Q1/045 , C12Q1/04 , C12Q1/10 , C12Q1/34 , Y10S435/802 , Y10S435/968
摘要： The invention relates to a target microbe-specific medium for detecting the presence or absence of a target microbe in an environmental or biological sample, the medium including: a) an effective amount of vitamin, amino acid, element and salt ingredients operable to allow viability and log phase reproduction of the target microbe in the presence of a nutrient-indicator and to aid the target microbe through lag phase and into log phase of growth in the sample; and b) an effective amount of a nutrient-indicator which is provided in an amount sufficient to support log phase growth of the target microbe of a sample until a detectable characteristic signal is produced in the medium/sample mixture during the log phase growth; the nutrient-indicator being incapable of supporting continued logarithmic growth of any viable non-target microbes in the medium/sample mixture to produce a detectable characteristic signal; and the nutrient-indicator being operable to alter a detectable characteristic of the medium/sample mixture if metabolized by the target microbe so as to confirm the presence or absence of the target microbe in the sample; wherein the medium lacks a gelling agent so that when the medium is mixed with a liquefied sample a liquid is formed, and wherein the ingredients in (a), and the nutrient-indicator are chosen such that growth of non-target microbes do not interfere with growth of the target microbe. The invention also relates to methods of detecting the presence or absence of a target microbe in which an environmental or liquid sample is mixed with the medium, and the mixture is then evaluated for the alteration of a detectable characteristic.
摘要翻译：本发明涉及用于检测环境或生物样品中目标微生物的存在或不存在的靶微生物特异性培养基，所述培养基包括：a）有效量的维生素，氨基酸，元素和盐成分，其可操作以允许存活力并在存在营养指标的情况下对目标微生物进行对数期再生，并帮助目标微生物通过滞后期和样品生长的对数期; 和b）有效量的营养指示剂，其以足以支持样品的目标微生物的对数生长的量提供，直到在对数生长期间在培养基/样品混合物中产生可检测的特征信号; 营养指标不能支持培养基/样品混合物中任何活的非目标微生物的持续对数生长，以产生可检测的特征信号; 并且所述营养指示剂可操作以改变所述培养基/样品混合物的可检测特性，如果被所述靶微生物代谢，以便确认样品中目标微生物的存在或不存在; 其中所述介质缺少胶凝剂，使得当所述介质与液化样品混合时，形成液体，并且其中选择（a）中的成分和所述营养指示剂使得非目标微生物的生长不会干扰随着目标微生物的增长。本发明还涉及检测其中将环境或液体样品与培养基混合的目标微生物的存在或不存在的方法，然后评估混合物以改变可检测特征。

8. 发明专利

DE3687232T3 未知
公开(公告)号：DE3687232T3
公开(公告)日：2000-09-07
申请号：DE3687232
申请日：1986-12-19
申请人： EDBERG STEPHEN C
发明人： EDBERG STEPHEN C
IPC分类号： C12N1/20 , C12Q1/04 , C12Q1/10 , C12Q1/34 , C12Q1/37 , C12R1/19

9. 发明专利

AU5911099A Modulation of microbial metabolism and/or growth rate by plant growth hormones 未知
公开(公告)号：AU5911099A
公开(公告)日：2000-03-27
申请号：AU5911099
申请日：1999-09-07
申请人： EDBERG STEPHEN C
发明人： EDBERG STEPHEN C
IPC分类号： C12N1/14 , C12N1/20 , C12N1/38 , C12Q1/04 , C12Q1/18 , C12Q1/02 , C12N1/16

10. 发明专利

DK175229B1 未知
公开(公告)号：DK175229B1
公开(公告)日：2004-07-19
申请号：DK332687
申请日：1987-06-29
申请人： EDBERG STEPHEN C
发明人： EDBERG STEPHEN C
IPC分类号： C12N1/20 , C12Q1/04 , C12Q1/10 , C12Q1/34 , C12Q1/37 , C12R1/19

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