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1. 发明授权

US08222005B2 Method for gene identification signature (GIS) analysis 有权
标题翻译：基因鉴别特征（GIS）分析方法
公开(公告)号：US08222005B2
公开(公告)日：2012-07-17
申请号：US10664234
申请日：2003-09-17
申请人： Yijun Ruan , Patrick Ng , Chialin Wei
发明人： Yijun Ruan , Patrick Ng , Chialin Wei
IPC分类号： C12P19/34 , C12N15/66 , G01N33/50
CPC分类号： C12Q1/6809 , C12N15/1065 , C12N15/1093 , C12N15/1096 , C12Q1/6855 , C12Q2525/191 , C12Q2525/131 , C12Q2521/313
摘要： An isolated oligonucleotide comprising at least one ditag, wherein the ditag comprises two joined first and second sequence tags, wherein the first tag comprises the 5′-terminus sequence and the second tag comprises the 3′-terminus sequence of a nucleic acid molecule or a fragment thereof. The ditag analysis is useful for gene discovery and genome mapping.
摘要翻译：包含至少一个ditag的分离的寡核苷酸，其中所述ditag包含两个连接的第一和第二序列标签，其中所述第一标签包含5'-末端序列，并且所述第二标签包含核酸分子的3'-末端序列或片段。 ditag分析可用于基因发现和基因组图谱。

2. 发明申请

US20060084083A1 Method to generate or determine nucleic acid tags corresponding to the terminal ends of DNA molecules using sequences analysis of gene expression (terminal SAGE) 有权
标题翻译：使用基因表达序列分析（终端SAGE）产生或确定对应于DNA分子末端的核酸标签的方法
公开(公告)号：US20060084083A1
公开(公告)日：2006-04-20
申请号：US11145005
申请日：2005-06-03
申请人： Yijun Ruan , Chialin Wei
发明人： Yijun Ruan , Chialin Wei
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6809 , C12Q2539/103
摘要： A method of providing an indication of an instance of expression of a gene is described herein, the method which may comprise the steps of: (a) providing a complementary deoxyribonucleic acid (cDNA) having a terminus comprising a terminal transcribed sequence of a gene; (b) linking the cDNA to an linker sequence thereby forming a linked nucleic acid, in which the linker sequence comprises a first recognition site for a first nucleic acid cleavage enzyme, preferably a restriction endonuclease, that allows nucleic acid cleavage at a site distant from the first recognition site; and (c) cleaving the linked nucleic acid with the first nucleic acid cleavage enzyme to provide a linked tag, in which the linked tag comprises a nucleotide sequence tag representative of a terminal transcribed sequence of the gene; and (d) detecting the presence or identity of the linked tag or the nucleotide sequence tag to provide an indication of an instance of gene expression.
摘要翻译：本文描述了提供基因表达实例的指示的方法，所述方法可以包括以下步骤：（a）提供具有包含基因的末端转录序列的末端的互补脱氧核糖核酸（cDNA）（b）将cDNA连接到接头序列，从而形成连接的核酸，其中接头序列包含用于第一核酸切割酶的第一识别位点，优选限制性内切核酸酶，其允许在远离的位点进行核酸切割第一个认可网站; 和（c）用第一核酸切割酶切割连接的核酸以提供连接的标签，其中连接的标签包含代表该基因的末端转录序列的核苷酸序列标签; 和（d）检测连锁标签或核苷酸序列标签的存在或身份以提供基因表达实例的指示。

3. 发明授权

US08263367B2 Nucleic acid interaction analysis 有权
标题翻译：核酸相互作用分析
公开(公告)号：US08263367B2
公开(公告)日：2012-09-11
申请号：US12020071
申请日：2008-01-25
申请人： Yijun Ruan , Chialin Wei , Melissa Jane Fullwood , Jun Liu
发明人： Yijun Ruan , Chialin Wei , Melissa Jane Fullwood , Jun Liu
IPC分类号： C07H22/00 , C12P19/34
CPC分类号： C12N15/1065 , C12Q1/6804 , C12Q1/6806 , C12Q2525/191 , C12Q2525/186 , C12Q2523/101
摘要： The present invention provides at least one isolated linear composite nucleic acid molecule comprising at least one first tag from at least one first nucleic acid molecule and at least one second tag from at least one second nucleic acid molecule, wherein the first and second nucleic acids interact in a nucleic acid mixture; and wherein the first and second tags are from different nucleic acid molecules. The invention also provides a method of producing at least one isolated linear composite nucleic acid and to a method of detecting and/or identifying nucleic acid interactions.
摘要翻译：本发明提供至少一种分离的线性复合核酸分子，其包含来自至少一个第一核酸分子的至少一个第一标签和至少一个第二核酸分子的至少一个第二标签，其中所述第一和第二核酸相互作用在核酸混合物中; 并且其中所述第一和第二标签来自不同的核酸分子。本发明还提供了生产至少一种分离的线性复合核酸的方法以及检测和/或鉴定核酸相互作用的方法。

4. 发明申请

US20090191598A1 Nucleic Acid Interaction Analysis 有权
标题翻译：核酸相互作用分析
公开(公告)号：US20090191598A1
公开(公告)日：2009-07-30
申请号：US12020071
申请日：2008-01-25
申请人： Yijun Ruan , Chialin Wei , Melissa Jane Fullwood , Jun Liu
发明人： Yijun Ruan , Chialin Wei , Melissa Jane Fullwood , Jun Liu
IPC分类号： C07H21/00 , C12P19/34
CPC分类号： C12N15/1065 , C12Q1/6804 , C12Q1/6806 , C12Q2525/191 , C12Q2525/186 , C12Q2523/101
摘要： The present invention provides at least one isolated linear composite nucleic acid molecule comprising at least one first tag from at least one first nucleic acid molecule and at least one second tag from at least one second nucleic acid molecule, wherein the first and second nucleic acids interact in a nucleic acid mixture; and wherein the first and second tags are from different nucleic acid molecules. The invention also provides a method of producing at least one isolated linear composite nucleic acid and to a method of detecting and/or identifying nucleic acid interactions.
摘要翻译：本发明提供至少一种分离的线性复合核酸分子，其包含来自至少一个第一核酸分子的至少一个第一标签和至少一个第二核酸分子的至少一个第二标签，其中所述第一和第二核酸相互作用在核酸混合物中; 并且其中所述第一和第二标签来自不同的核酸分子。本发明还提供了生产至少一种分离的线性复合核酸的方法以及检测和/或鉴定核酸相互作用的方法。

5. 发明授权

US07553947B2 Method for gene identification signature (GIS) analysis 有权
标题翻译：基因鉴别特征（GIS）分析方法
公开(公告)号：US07553947B2
公开(公告)日：2009-06-30
申请号：US11610118
申请日：2006-12-13
申请人： Patrick Ng , Chialin Wei , Yijun Ruan
发明人： Patrick Ng , Chialin Wei , Yijun Ruan
IPC分类号： C12N15/11 , C12N15/63 , C07H21/04
CPC分类号： C12Q1/6809 , C12N15/1065 , C12N15/1093 , C12N15/1096 , C12Q1/6855 , C12Q2525/191 , C12Q2525/131 , C12Q2521/313
摘要： A method of identifying at least a nucleic acid molecule fragment to which a protein of interest binds, comprising: (i) preparing at least one nucleic acid molecule fragment to which a protein binds; (ii) isolating the 5′ terminus and the 3′ terminus of the nucleic acid fragment(s) and linking the 5′ terminus and 3′ terminus to create the at least one ditag; (iii) sequencing the ditag; and (iv) mapping the ditag sequence(s) to the genome.
摘要翻译：鉴定至少一个目标蛋白质所结合的核酸分子片段的方法，包括：（i）制备蛋白质结合至少一个核酸分子片段; （ii）分离核酸片段的5'末端和3'末端并连接5'末端和3'末端以产生至少一个ditag; （iii）测序ditag; 和（iv）将分离序列映射到基因组。

6. 发明申请

US20070161024A1 Method for Gene Identification Signature (GIS) Analysis 有权
标题翻译：基因鉴别特征（GIS）分析方法
公开(公告)号：US20070161024A1
公开(公告)日：2007-07-12
申请号：US11610118
申请日：2006-12-13
申请人： Patrick Ng , Chialin Wei , Yijun Ruan
发明人： Patrick Ng , Chialin Wei , Yijun Ruan
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/6809 , C12N15/1065 , C12N15/1093 , C12N15/1096 , C12Q1/6855 , C12Q2525/191 , C12Q2525/131 , C12Q2521/313
摘要： A method of identifying at least a nucleic acid molecule fragment to which a protein of interest binds, comprising: (i) preparing at least one nucleic acid molecule fragment to which a protein binds; (ii) isolating the 5′ terminus and the 3′ terminus of the nucleic acid fragment(s) and linking the 5′ terminus and 3′ terminus to create the at least one ditag; (iii) sequencing the ditag; and (iv) mapping the ditag sequence(s) to the genome.
摘要翻译：鉴定至少一个目标蛋白质所结合的核酸分子片段的方法，包括：（i）制备蛋白质结合至少一个核酸分子片段; （ii）分离核酸片段的5'末端和3'末端并连接5'末端和3'末端以产生至少一个ditag; （iii）测序ditag; 和（iv）将分离序列映射到基因组。

7. 发明申请

US20120214157A1 METHOD TO GENERATE OR DETERMINE NUCLEIC ACID TAGS CORRESPONDING TO THE TERMINAL ENDS OF DNA MOLECULES USING SEQUENCES ANALYSIS OF GENE EXPRESSION (TERMINAL SAGE) 审中-公开
标题翻译：使用序列分析基因表达（终端信号）生成或确定与DNA分子末端相关的核酸标签的方法
公开(公告)号：US20120214157A1
公开(公告)日：2012-08-23
申请号：US13407792
申请日：2012-02-29
申请人： Yijun Ruan , Chialin Wei
发明人： Yijun Ruan , Chialin Wei
IPC分类号： C12Q1/68 , G06F19/28
CPC分类号： C12Q1/6809 , C12Q2539/103
摘要： We describe a method of providing an indication of an instance of expression of a gene, the method comprising the steps of: (a) providing a complementary deoxyribonucleic acid (cDNA) having a terminus comprising a terminal transcribed sequence of a gene; (b) linking the cDNA to an linker sequence thereby forming a linked nucleic acid, in which the linker sequence comprises a first recognition site for a first nucleic acid cleavage enzyme, preferably a restriction endonuclease, that allows nucleic acid cleavage at a site distant from the first recognition site; and (c) cleaving the linked nucleic acid with the first nucleic acid cleavage enzyme to provide a linked tag, in which the linked tag comprises a nucleotide sequence tag representative of a terminal transcribed sequence of the gene; and (d) detecting the presence or identity of the linked tag or the nucleotide sequence tag to provide an indication of an instance of gene expression.
摘要翻译：我们描述了提供基因表达实例的指示的方法，所述方法包括以下步骤：（a）提供具有包含基因的末端转录序列的末端的互补脱氧核糖核酸（cDNA）（b）将cDNA连接到接头序列，从而形成连接的核酸，其中接头序列包含用于第一核酸切割酶的第一识别位点，优选限制性内切核酸酶，其允许在远离的位点进行核酸切割第一个认可网站; 和（c）用第一核酸切割酶切割连接的核酸以提供连接的标签，其中连接的标签包含代表该基因的末端转录序列的核苷酸序列标签; 和（d）检测连锁标签或核苷酸序列标签的存在或身份以提供基因表达实例的指示。

8. 发明授权

US08158355B2 Method to generate or determine nucleic acid tags corresponding to the terminal ends of DNA molecules using sequences analysis of gene expression (terminal SAGE) 有权
标题翻译：使用基因表达序列分析（终端SAGE）产生或确定对应于DNA分子末端的核酸标签的方法
公开(公告)号：US08158355B2
公开(公告)日：2012-04-17
申请号：US11145005
申请日：2005-06-03
申请人： Yijun Ruan , Chialin Wei
发明人： Yijun Ruan , Chialin Wei
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q2539/103
摘要： A method of providing an indication of an instance of expression of a gene is described herein, the method which may comprise the steps of: (a) providing a complementary deoxyribonucleic acid (cDNA) having a terminus comprising a terminal transcribed sequence of a gene; (b) linking the cDNA to an linker sequence thereby forming a linked nucleic acid, in which the linker sequence comprises a first recognition site for a first nucleic acid cleavage enzyme, preferably a restriction endonuclease, that allows nucleic acid cleavage at a site distant from the first recognition site; and (c) cleaving the linked nucleic acid with the first nucleic acid cleavage enzyme to provide a linked tag, in which the linked tag comprises a nucleotide sequence tag representative of a terminal transcribed sequence of the gene; and (d) detecting the presence or identity of the linked tag or the nucleotide sequence tag to provide an indication of an instance of gene expression.
摘要翻译：本文描述了提供基因表达实例的指示的方法，所述方法可以包括以下步骤：（a）提供具有包含基因的末端转录序列的末端的互补脱氧核糖核酸（cDNA）（b）将cDNA连接到接头序列，从而形成连接的核酸，其中接头序列包含用于第一核酸切割酶的第一识别位点，优选限制性内切核酸酶，其允许在远离的位点进行核酸切割第一个认可网站; 和（c）用第一核酸切割酶切割连接的核酸以提供连接的标签，其中连接的标签包含代表该基因的末端转录序列的核苷酸序列标签; 和（d）检测连锁标签或核苷酸序列标签的存在或身份以提供基因表达实例的指示。

9. 发明申请

US20060084111A1 Method for gene identification signature (GIS) analysis 审中-公开
标题翻译：基因鉴别特征（GIS）分析方法
公开(公告)号：US20060084111A1
公开(公告)日：2006-04-20
申请号：US11304538
申请日：2005-12-16
申请人： Yijun Ruan , Patrick Ng , Chialin Wei
发明人： Yijun Ruan , Patrick Ng , Chialin Wei
IPC分类号： C40B40/08 , C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6809 , C12N15/1065 , C12N15/1093 , C12N15/1096 , C12Q1/6855 , C12Q2525/191 , C12Q2525/131 , C12Q2521/313
摘要： An isolated oligonucleotide comprising at least one ditag, wherein the ditag comprises two joined first and second sequence tags, wherein the first tag comprises the 5′-terminus sequence and the second tag comprises the 3′-terminus sequence of a nucleic acid molecule or a fragment thereof. The ditag analysis is useful for gene discovery and genome mapping.
摘要翻译：包含至少一个ditag的分离的寡核苷酸，其中所述ditag包含两个连接的第一和第二序列标签，其中所述第一标签包含5'-末端序列，并且所述第二标签包含核酸分子的3'-末端序列或片段。 ditag分析可用于基因发现和基因组图谱。

10. 发明申请

US20050255501A1 Method for gene identification signature (GIS) analysis 审中-公开
标题翻译：基因鉴别特征（GIS）分析方法
公开(公告)号：US20050255501A1
公开(公告)日：2005-11-17
申请号：US11045468
申请日：2005-01-31
申请人： Patrick Ng , Chialin Wei , Yijun Ruan
发明人： Patrick Ng , Chialin Wei , Yijun Ruan
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6809 , C12N15/1065 , C12N15/1093 , C12N15/1096 , C12Q1/6855 , C12Q2525/191 , C12Q2525/131 , C12Q2521/313
摘要： A method of identifying at least a nucleic acid molecule fragment to which a protein of interest binds, comprising: (i) preparing at least one nucleic acid molecule fragment to which a protein binds; (ii) isolating the 5′ terminus and the 3′ terminus of the nucleic acid fragment(s) and linking the 5′ terminus and 3′ terminus to create the at least one ditag; (iii) sequencing the ditag; and (iv) mapping the ditag sequence(s) to the genome.
摘要翻译：鉴定至少一个目标蛋白质所结合的核酸分子片段的方法，包括：（i）制备蛋白质结合至少一个核酸分子片段; （ii）分离核酸片段的5'末端和3'末端并连接5'末端和3'末端以产生至少一个ditag; （iii）测序ditag; 和（iv）将分离序列映射到基因组。

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