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1. 发明申请

US20100148126A1 GENOMIC SEQUENCING USING MODIFIED PROTEIN PORES AND IONIC LIQUIDS 审中-公开
标题翻译：使用改性蛋白质孔和离子液体进行基因测序
公开(公告)号：US20100148126A1
公开(公告)日：2010-06-17
申请号：US12625207
申请日：2009-11-24
申请人： Xiyun Guan , Dilani A. Jayawardhana , Daniel W. Armstrong , Ranulu Samanthi De Zoysa , Deqiang Wang , Qitao Zhao
发明人： Xiyun Guan , Dilani A. Jayawardhana , Daniel W. Armstrong , Ranulu Samanthi De Zoysa , Deqiang Wang , Qitao Zhao
IPC分类号： H01B1/12 , C07D233/00 , C07K1/00 , G01N31/00 , G01R27/08
CPC分类号： G01N33/84 , C07D233/06 , C12Q1/02 , C12Q1/6869 , G01N33/6803 , G01N33/6872 , C12Q2565/631 , C12Q2565/607
摘要： The present invention is a nanopore stochastic sensing system comprising modified protein pores for detection and sequencing of oligonucleotides. The system comprises a genetically modified protein pore with a variety of non-covalent bonding recognition sites to significantly slow down the translocation of ssDNA in the pores. The present invention also describes identification and application of DNA fingerprints, which are a sequence of small current modulation events for the determination of the sequence of ssDNA molecules. In separate embodiments the present invention describes a system and a method for the detection of monovalent cations, liquid explosives, water-insoluble compounds, biomolecules and oligonucleotides. The system comprising a wild-type or genetically modified protein pore with or without a molecular adaptor. Analyte samples and mixtures are added along with specially synthesized ionic liquids.
摘要翻译：本发明是一种纳米孔随机感测系统，其包含用于寡核苷酸检测和测序的修饰蛋白质孔。该系统包括具有多种非共价键合识别位点的经遗传修饰的蛋白质孔，以显着减慢细胞中ssDNA的易位。本发明还描述了DNA指纹的鉴定和应用，DNA指纹是用于测定ssDNA分子序列的小电流调制事件的序列。在单独的实施方案中，本发明描述了用于检测一价阳离子，液体炸药，水不溶性化合物，生物分子和寡核苷酸的系统和方法。该系统包含具有或不具有分子衔接子的野生型或经遗传修饰的蛋白质孔。分析物样品和混合物与特殊合成的离子液体一起加入。

2. 发明申请

US20100099198A1 APPARATUS AND SYSTEM FOR PATTERN RECOGNITION SENSING FOR BIOMOLECULES 审中-公开
标题翻译：用于生物分子模式识别的装置和系统
公开(公告)号：US20100099198A1
公开(公告)日：2010-04-22
申请号：US12499725
申请日：2009-07-08
申请人： Qitao Zhao , Dilani A. Jayawardhana , Xiyun Guan
发明人： Qitao Zhao , Dilani A. Jayawardhana , Xiyun Guan
IPC分类号： G01N27/416 , C12M1/00 , H01R43/00
CPC分类号： G01N33/48721 , Y10T29/49117
摘要： The present invention is an array nanopore stochastic sensing system for detection of single biomolecules and oligonucleotides. The system comprises a multi-channel system with multiple genetically modified protein pores for detection of analytes using the pattern recognition mechanism. By monitoring current blockade patterns, identity of single biomolecules can be determined in complex mixtures.
摘要翻译：本发明是用于检测单个生物分子和寡核苷酸的阵列纳米孔随机感测系统。该系统包括具有多个经遗传修饰的蛋白质孔的多通道系统，用于使用模式识别机制检测分析物。通过监测当前的阻断模式，可以在复杂的混合物中确定单个生物分子的身份。

3. 发明申请

US20150259724A1 NANOPORE STOCHASTIC SENSING OF BIOMARKERS 有权
标题翻译： NANOPORE生物标志物的STOCHASTIC SENSING
公开(公告)号：US20150259724A1
公开(公告)日：2015-09-17
申请号：US14657810
申请日：2015-03-13
申请人： Xiyun Guan , Liang Wang , Shuo Zhou
发明人： Xiyun Guan , Liang Wang , Shuo Zhou
IPC分类号： C12Q1/37 , C12Q1/68 , G01N33/487
CPC分类号： C12Q1/37 , C12Q1/6825 , G01N33/48721 , C12Q2565/631
摘要： A method and system for sensing or characterizing a biomarker, such as a proteolytic enzyme or nucleic acid. The system comprises a nanopore sensor to determine a current modulation of a sample including a biomarker, and a predetermined substrate or nucleic acid probe current modulation signature for comparison to a current signature from the nanopore sensor. The nanopore sensor includes a nanopore membrane between two fluid compartments, and a power supply in electrical contact with the membrane to provide an electric potential difference between the fluid compartments. A detector is used to detect an electrical current through the nanopore as the polypeptide substrate, or components thereof, transits the nanopore under an applied electric potential difference between the first and second fluid compartments. The result is a rapid, label-free method for the sensitive and accurate measurement of biomarker activity by real-time monitoring of the ionic current modulations arising from the substrate peptide-protease interactions or nucleic acid hybridization in the nanopore.
摘要翻译：用于感测或表征生物标志物如蛋白水解酶或核酸的方法和系统。该系统包括纳米孔传感器，以确定包含生物标志物的样品的当前调制，以及用于与来自纳米孔传感器的当前特征进行比较的预定底物或核酸探针电流调制特征。纳米孔传感器包括在两个流体室之间的纳米孔膜，以及与膜电接触以提供流体室之间的电位差的电源。检测器用于检测通过纳米孔的电流，因为多肽底物或其组分在第一和第二流体隔室之间施加的电位差下转移纳米孔。结果是通过实时监测由纳米孔中的底物肽 - 蛋白酶相互作用或核酸杂交产生的离子电流调节，快速，无标记的方法灵敏和准确地测量生物标志物活性。

4. 发明授权

US09828623B2 Nanopore stochastic sensing of biomarkers 有权
公开(公告)号：US09828623B2
公开(公告)日：2017-11-28
申请号：US14657810
申请日：2015-03-13
申请人： Xiyun Guan , Liang Wang , Shuo Zhou
发明人： Xiyun Guan , Liang Wang , Shuo Zhou
IPC分类号： C12Q1/37 , G01N33/487 , C12Q1/68
CPC分类号： C12Q1/37 , C12Q1/6825 , G01N33/48721 , C12Q2565/631
摘要： A method and system for sensing or characterizing a biomarker, such as a proteolytic enzyme or nucleic acid. The system comprises a nanopore sensor to determine a current modulation of a sample including a biomarker, and a predetermined substrate or nucleic acid probe current modulation signature for comparison to a current signature from the nanopore sensor. The nanopore sensor includes a nanopore membrane between two fluid compartments, and a power supply in electrical contact with the membrane to provide an electric potential difference between the fluid compartments. A detector is used to detect an electrical current through the nanopore as the polypeptide substrate, or components thereof, transits the nanopore under an applied electric potential difference between the first and second fluid compartments. The result is a rapid, label-free method for the sensitive and accurate measurement of biomarker activity by real-time monitoring of the ionic current modulations arising from the substrate peptide-protease interactions or nucleic acid hybridization in the nanopore.

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