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1. 发明申请

US20100173364A1 Repair of Nucleic Acids for Improved Amplification 审中-公开
标题翻译：用于改进扩增的核酸修复
公开(公告)号：US20100173364A1
公开(公告)日：2010-07-08
申请号：US12296827
申请日：2007-04-11
申请人： Thomas C. Evans, JR. , Barton Slatko , Lixin Chen , Romaldas Vaisvila , Chudi Guan , Rebecca Kucera
发明人： Thomas C. Evans, JR. , Barton Slatko , Lixin Chen , Romaldas Vaisvila , Chudi Guan , Rebecca Kucera
IPC分类号： C12P19/34 , C12N9/00 , C12N9/10
CPC分类号： C12Q1/686 , C12Q2521/514 , C12Q2521/501 , C12Q2521/301 , C12Q2525/119
摘要： Methods and compositions are provided for repairing a polynucleotide so that it can be copied with improved fidelity and/o yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a DNA ligase and an effective amount of at least one endonuclease as well as a cofactor selected from NAD+ or ATP.
摘要翻译：提供了用于修复多核苷酸的方法和组合物，使得其可以在例如扩增反应中以改进的保真度和/或产率复制。这包括使用包括DNA连接酶和有效量的至少一种内切核酸酶以及选自NAD +或ATP的辅因子的反应混合物。

2. 发明授权

US07700283B2 Repair of nucleic acids for improved amplification 有权
标题翻译：修复核酸以改善扩增
公开(公告)号：US07700283B2
公开(公告)日：2010-04-20
申请号：US11255290
申请日：2005-10-20
申请人： Thomas C. Evans , Barton Slatko , Lixin Chen , Romaldas Vaisvila , Chudi Guan
发明人： Thomas C. Evans , Barton Slatko , Lixin Chen , Romaldas Vaisvila , Chudi Guan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6844 , C12Q1/6848 , C12Q2521/501 , C12Q2521/301 , C12Q2521/514 , C12Q2521/101 , C12Q2521/331
摘要： Methods and compositions are provided for repairing a polynucleotide so that it can be synthesized efficiently with improved fidelity and yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a ligase and a cofactor selected from NAD+ or ATP and incubating the polynucleotide with the reaction mixture in the absence of Endonuclease VI.The reaction mixture may further contain an AP endonuclease and a polymerase. These enzymes are optionally selected according to their ability to withstand high temperatures so they can be included in an amplification mixture. The reaction mixture may be used prior to a polynucleotide synthesis reaction in which case enzymes that are not thermophilic may be used. The repair reaction is not time sensitive with respect to seconds, minutes or hours of incubation in the enzyme mixture.
摘要翻译：提供了用于修复多核苷酸的方法和组合物，使得其可以在例如扩增反应中以提高的保真度和产率被有效地合成。这包括使用包含连接酶和选自NAD +或ATP的辅因子并在不存在核酸内切酶VI的情况下将多核苷酸与反应混合物一起温育的反应混合物。反应混合物还可以含有AP内切核酸酶和聚合酶。这些酶根据其耐受高温的能力任选地进行选择，因此它们可以被包括在扩增混合物中。反应混合物可以在多核苷酸合成反应之前使用，在这种情况下可以使用不是嗜热的酶。相对于在酶混合物中孵育的秒，分钟或数小时，修复反应不是时间敏感的。

3. 发明申请

US20060088868A1 Repair of nucleic acids for improved amplification 有权
标题翻译：修复核酸以改善扩增
公开(公告)号：US20060088868A1
公开(公告)日：2006-04-27
申请号：US11255290
申请日：2005-10-20
申请人： Thomas Evans , Barton Slatko , Lixin Chen , Romaldas Vaisvila , Chudi Guan
发明人： Thomas Evans , Barton Slatko , Lixin Chen , Romaldas Vaisvila , Chudi Guan
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , C12Q1/6844 , C12Q1/6848 , C12Q2521/501 , C12Q2521/301 , C12Q2521/514 , C12Q2521/101 , C12Q2521/331
摘要： Methods and compositions are provided for repairing a polynucleotide so that it can be synthesized efficiently with improved fidelity and yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a ligase and a cofactor selected from NAD+ or ATP and incubating the polynucleotide with the reaction mixture in the absence of Endonuclease VI. The reaction mixture may further contain an AP endonuclease and a polymerase. These enzymes are optionally selected according to their ability to withstand high temperatures so they can be included in an amplification mixture. The reaction mixture may be used prior to a polynucleotide synthesis reaction in which case enzymes that are not thermophilic may be used. The repair reaction is not time sensitive with respect to seconds, minutes or hours of incubation in the enzyme mixture.
摘要翻译：提供了用于修复多核苷酸的方法和组合物，使得其可以在例如扩增反应中以提高的保真度和产率被有效地合成。这包括使用包含连接酶和选自NAD +或ATP的辅因子并在不存在核酸内切酶VI的情况下将多核苷酸与反应混合物一起温育的反应混合物。反应混合物还可以含有AP内切核酸酶和聚合酶。这些酶根据其耐受高温的能力任选地进行选择，因此它们可以被包括在扩增混合物中。反应混合物可以在多核苷酸合成反应之前使用，在这种情况下可以使用不是嗜热的酶。相对于在酶混合物中孵育的秒，分钟或数小时，修复反应不是时间敏感的。

4. 发明申请

US20060177867A1 Repair of nucleic acids for improved amplification 有权
标题翻译：修复核酸以改善扩增
公开(公告)号：US20060177867A1
公开(公告)日：2006-08-10
申请号：US11401826
申请日：2006-04-11
申请人： Thomas Evans , Barton Slatko , Lixin Chen , Romaldas Vaisvila , Chudi Guan , Rebecca Kucera
发明人： Thomas Evans , Barton Slatko , Lixin Chen , Romaldas Vaisvila , Chudi Guan , Rebecca Kucera
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q2521/101 , C12Q2521/501 , C12Q2521/331 , C12Q2521/301 , C12Q2521/514
摘要： Methods and compositions are provided for repairing a polynucleotide so that it can be copied with improved fidelity and/or yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a ligase and a cofactor selected from NAD+ or ATP and incubating the polynucleotide with the reaction mixture in the absence of Endonuclease VI. The reaction mixture may further contain an AP endonuclease and a polymerase. If used, these enzymes may be selected according to their ability to withstand high temperatures. For example, the reaction mixture may be used prior to a polynucleotide synthesis reaction in which case enzymes that are not thermophilic may be used. The repair reaction is not time sensitive with respect to seconds, minutes or hours of incubation in the enzyme mixture in as much as the repair is effected rapidly and prolonged incubation is not generally adverse.
摘要翻译：提供了用于修复多核苷酸的方法和组合物，使得其可以在例如扩增反应中以改进的保真度和/或产率复制。这包括使用包含连接酶和选自NAD +或ATP的辅因子的反应混合物，并在不存在核酸内切酶VI的情况下将多核苷酸与反应混合物一起温育。反应混合物还可以含有AP内切核酸酶和聚合酶。如果使用，这些酶可以根据其耐受高温的能力来选择。例如，反应混合物可以在多核苷酸合成反应之前使用，在这种情况下可以使用不是嗜热的酶。维修反应对于酶混合物中的几秒钟，几分钟或几小时的孵育时间并不敏感，只要修复迅速进行并且延长的孵育通常不是不利的。

5. 发明授权

US08158388B2 Repair of nucleic acids for improved amplification 有权
标题翻译：修复核酸以改善扩增
公开(公告)号：US08158388B2
公开(公告)日：2012-04-17
申请号：US11401826
申请日：2006-04-11
申请人： Thomas C. Evans , Lixin Chen , Chudi Guan , Rebecca Kucera , Barton Slatko , Romualdas Vaisvila
发明人： Thomas C. Evans , Lixin Chen , Chudi Guan , Rebecca Kucera , Barton Slatko , Romualdas Vaisvila
IPC分类号： C12P19/34
CPC分类号： C12Q1/6844 , C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q2521/101 , C12Q2521/501 , C12Q2521/331 , C12Q2521/301 , C12Q2521/514
摘要： Methods and compositions are provided for repairing a polynucleotide so that it can be copied with improved fidelity and/or yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a ligase and a cofactor selected from NAD+ or ATP and incubating the polynucleotide with the reaction mixture in the absence of Endonuclease VI.The reaction mixture may further contain an AP endonuclease and a polymerase. If used, these enzymes may be selected according to their ability to withstand high temperatures. For example, the reaction mixture may be used prior to a polynucleotide synthesis reaction in which case enzymes that are not thermophilic may be used. The repair reaction is not time sensitive with respect to seconds, minutes or hours of incubation in the enzyme mixture in as much as the repair is effected rapidly and prolonged incubation is not generally adverse.
摘要翻译：提供了用于修复多核苷酸的方法和组合物，使得其可以在例如扩增反应中以改进的保真度和/或产率复制。这包括使用包含连接酶和选自NAD +或ATP的辅因子并在不存在核酸内切酶VI的情况下将多核苷酸与反应混合物一起温育的反应混合物。反应混合物还可以含有AP内切核酸酶和聚合酶。如果使用，这些酶可以根据其耐受高温的能力来选择。例如，反应混合物可以在多核苷酸合成反应之前使用，在这种情况下可以使用不是嗜热的酶。维修反应对于酶混合物中的几秒钟，几分钟或几小时的孵育时间并不敏感，只要修复迅速进行并且延长的孵育通常不是不利的。

6. 发明授权

US06858775B1 Method for generating split, non-transferable genes that are able to express an active protein product 有权
标题翻译：用于产生能够表达活性蛋白质产物的分裂的，不可转移的基因的方法
公开(公告)号：US06858775B1
公开(公告)日：2005-02-22
申请号：US09936588
申请日：2000-05-23
申请人： Ming-Qun Xu , Thomas C. Evans , Sriharsa Pradhan , Donald G. Comb , Henry Paulus , Luo Sun , Lixin Chen , Inca Ghosh
发明人： Ming-Qun Xu , Thomas C. Evans , Sriharsa Pradhan , Donald G. Comb , Henry Paulus , Luo Sun , Lixin Chen , Inca Ghosh
IPC分类号： C07K14/435 , C12N9/10 , C12N9/12 , C12N9/88 , C12N15/10 , C12N15/62 , C12N15/82 , C12P21/02 , A01H1/00
CPC分类号： C12N9/1029 , C07K14/43595 , C07K2319/00 , C07K2319/055 , C07K2319/06 , C07K2319/08 , C07K2319/09 , C07K2319/32 , C07K2319/75 , C07K2319/92 , C12N9/1092 , C12N9/1252 , C12N9/88 , C12N15/10 , C12N15/62 , C12N15/8214 , C12N15/8216 , C12N15/8241 , C12N15/8242 , C12N15/8257 , C12N15/8275 , C12N15/8278 , C12P21/02
摘要： A new type of transgene system is disclosed which allows efficient protein expression in a target host such as a plant, but avoids the undesirable result of the migration of the transgene into related host system and/or to the environment via the pollen. The methods described herein may also be applied to the expression of virtually any protein of interest (e.g. a toxic protein) in eukaryotic (yeast, insect, mammalian cells, etc.) and prokaryotic (E. coli, etc.) organisms.
摘要翻译：公开了一种新型的转基因系统，其允许在靶宿主如植物中有效的蛋白质表达，但避免了通过花粉将转基因迁移到相关宿主系统和/或环境中的不期望的结果。本文所述的方法也可应用于真核生物（酵母，昆虫，哺乳动物细胞等）和原核（大肠杆菌等）生物中几乎任何目标蛋白质（例如毒性蛋白质）的表达。

7. 发明申请

US20060081483A1 Hydrogen storage container and mixture therein 审中-公开
标题翻译：储氢容器及其中的混合物
公开(公告)号：US20060081483A1
公开(公告)日：2006-04-20
申请号：US11250412
申请日：2005-10-17
申请人： Changpin Chen , Lixin Chen , Qidong Wang
发明人： Changpin Chen , Lixin Chen , Qidong Wang
IPC分类号： B65B3/00
CPC分类号： B01D53/02 , B01D53/0415 , B01D2253/1126 , B01D2257/108 , B01D2258/01 , B01D2258/0208 , B01D2259/4143 , B01D2259/4525 , B01D2259/4541 , B01D2259/4566 , F17C11/005 , Y02E60/321
摘要： This invention relates to a new kind of hydrogen storage mixture and container thereof. This hydrogen storage container consists of a container casing, a hydrogen storage mixture and a valve. The hydrogen storage mixture loaded inside the container is made of hydrogen storage alloy granules and metal fibers and/or non-hydrogen-absorbing alloy fibers that do not absorb hydrogen. The non-hydrogen-absorbing fibers are dispersed among the hydrogen storage alloy granules and form a network structure. The non-hydrogen-absorbing fibers include non-hydrogen-absorbing metal fibers and/or non-hydrogen-absorbing alloy fibers, or their mixture. In the hydrogen storage mixture, the weight ratio of the non-hydrogen-absorbing fibers to the hydrogen storage alloys is about 0.01˜0.1. The hydrogen storage installation adopting the technology of this invention can effectively prevent the metal hydride granules from moving over a comparatively long distance and accumulating in some locations in the container during hydriding and dehydriding, and also can improve the thermal conductivity of the hydrogen storage mixture inside the container. The hydrogen storage container of this invention is easy to manufacture, safe to use, and low in cost. This invention can be used for the manufacture of hydrogen storage containers for hydrogen storage, transport, compression and purification.
摘要翻译：本发明涉及一种新型的储氢混合物及其容器。该储氢容器由容器壳体，储氢混合物和阀组成。装载在容器内的储氢混合物由储氢合金颗粒和不吸收氢的金属纤维和/或非吸氢合金纤维制成。非吸氢纤维分散在储氢合金颗粒中并形成网络结构。非吸氢纤维包括非吸氢金属纤维和/或非吸氢合金纤维或它们的混合物。在储氢混合物中，非吸氢纤维与储氢合金的重量比为约0.01〜0.1。采用本发明技术的氢存储装置可以有效地防止金属氢化物颗粒在氢化和脱氢过程中相对长的距离移动并积聚在容器中的某些位置，并且还可以提高储氢混合物内部的热导率容器本发明的储氢容器易于制造，使用安全，成本低。本发明可用于制造用于储氢，运输，压缩和净化的储氢容器。

8. 外观设计

USD1007302S1 Folding box 有权
公开(公告)号：USD1007302S1
公开(公告)日：2023-12-12
申请号：US29911086
申请日：2023-08-29
申请人： Lixin Chen
设计人： Lixin Chen
摘要： FIG. 1 is a front, top perspective view of a first embodiment of a folding box, showing my new design;
FIG. 2 is a rear, bottom perspective view thereof;
FIG. 3 is a front elevation view thereof;
FIG. 4 is a rear elevation view thereof
FIG. 5 is a left side elevation view thereof;
FIG. 6 is a right side elevation view thereof;
FIG. 7 is a top plan view thereof;
FIG. 8 is a bottom plan view thereof;
FIG. 9 is an enlarged view of detail “9” identified in FIG. 1;
FIG. 10 is another perspective view of the folding box, shown in an alternative position;
FIG. 11 is a front, top perspective view of a second embodiment of the folding box;
FIG. 12 is a rear, bottom perspective view thereof;
FIG. 13 is a front elevation view thereof;
FIG. 14 is a rear elevation view thereof;
FIG. 15 is a left side elevation view thereof;
FIG. 16 is a right side elevation view thereof;
FIG. 17 is a top plan view thereof;
FIG. 18 is a bottom plan view thereof;
FIG. 19 is an enlarged view of detail “19” identified in FIG. 11; and,
FIG. 20 is another perspective view of the folding box, shown in an alternative position.
The dash dot dash lines in FIGS. 1, 9, 11 and 19 are for the purpose of illustrating the enlarged view indicators and form no part of the claimed design.

9. 发明申请

US20090103974A1 BALL STUD SYSTEM FOR USE WITHIN A BALL JOINT 有权
标题翻译：球窝系统用于球联
公开(公告)号：US20090103974A1
公开(公告)日：2009-04-23
申请号：US11874450
申请日：2007-10-18
申请人： Christopher Dendis , Lixin Chen , Lars David Moravy
发明人： Christopher Dendis , Lixin Chen , Lars David Moravy
IPC分类号： F16C11/06
CPC分类号： B62D7/18 , F16B9/026 , F16B39/04 , Y10T403/32614 , Y10T403/32737
摘要： A ball stud system that provides a ball stud that will not loosen easily under the application of repetitive side loading on the ball portion of the stud. The ball stud is secured to the vehicle steering knuckle arm using the combination of a tapered nut and tapered washer. The tapered nut includes slots and the pin of the ball stud an aperture. A cotter pin may be placed through the slots and aperture in order to further prevent rotation of the nut.
摘要翻译：一种球螺栓系统，其提供在螺柱的球部分上施加重复的侧向载荷的情况下不容易松动的球杆螺栓。球螺栓使用锥形螺母和锥形垫圈的组合固定到车辆转向节臂上。锥形螺母包括槽，球的销钉为孔。开口销可以通过槽和孔放置，以进一步防止螺母旋转。

10. 发明授权

US09643645B2 Ball stud system for use within a ball joint 有权
公开(公告)号：US09643645B2
公开(公告)日：2017-05-09
申请号：US11874450
申请日：2007-10-18
申请人： Christopher Dendis , Lixin Chen , Lars David Moravy
发明人： Christopher Dendis , Lixin Chen , Lars David Moravy
IPC分类号： F16C11/00 , B62D7/18 , F16B9/02 , F16B39/04
CPC分类号： B62D7/18 , F16B9/026 , F16B39/04 , Y10T403/32614 , Y10T403/32737
摘要： A ball stud system that provides a ball stud that will not loosen easily under the application of repetitive side loading on the ball portion of the stud. The ball stud is secured to the vehicle steering knuckle arm using the combination of a tapered nut and tapered washer. The tapered nut includes slots and the pin of the ball stud an aperture. A cotter pin may be placed through the slots and aperture in order to further prevent rotation of the nut.

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