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    • 1. 发明申请
    • ULTRAMARINE FLUORESCENT PROTEIN
    • 超紫外线荧光蛋白
    • US20100167394A1
    • 2010-07-01
    • US12453000
    • 2008-08-01
    • Takeharu NagaiWataru TomosugiTomoki Matsuda
    • Takeharu NagaiWataru TomosugiTomoki Matsuda
    • C12N5/10C07K14/00C07H21/00C12N15/74
    • C07K14/43595C07K2319/60
    • The present invention provides an artificial mutant of GFP having a novel emission peak, i.e., a fluorescent protein having an emission peak at 424 nm comprising an amino acid sequence represented by SEQ ID NO: 1, in which each of the amino acid residues at the 66th position and the 175th position is replaced and at least one of the amino acid residues at the 72nd position and the 206th position is further replaced, or a fluorescent protein having an emission peak at 424 nm and a pH-independent fluorescence intensity, in which each of the amino acid residues at the 65th, 145th, 148th, 46th and/or 203rd positions is further substituted. The fluorescent protein of the invention emits fluorescence having an emission peak at 424 nm and can be visually distinguished by its ultramarine color from other fluorescent proteins. The fluorescent protein has a pH-independent fluorescence intensity which is not affected by pH changes.
    • 本发明提供具有新发射峰的GFP的人工突变体,即在424nm处具有发射峰的荧光蛋白,其包含由SEQ ID NO:1表示的氨基酸序列,其中每个氨基酸残基 第66位和第175位被置换,第72位和第206位的氨基酸残基中的至少一个被进一步置换,或者在424nm具有发射峰和不依赖于pH的荧光强度的荧光蛋白,其中 第65,第145,第148,第46和/或第203位的每个氨基酸残基被进一步取代。 本发明的荧光蛋白发射具有424nm处的发射峰的荧光,并且可以通过其来自其他荧光蛋白的群青颜色来目视辨别荧光。 荧光蛋白具有pH不依赖于荧光强度,不受pH变化的影响。