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1. 发明申请

US20110045009A1 Cell Permeable Peptide 有权
标题翻译：细胞渗透肽
公开(公告)号：US20110045009A1
公开(公告)日：2011-02-24
申请号：US11883810
申请日：2006-02-09
申请人： Shinsaku Nakagawa , Tadanori Mayumi , Kiichi Fukui
发明人： Shinsaku Nakagawa , Tadanori Mayumi , Kiichi Fukui
IPC分类号： A61K39/00 , C07K7/08 , A61P37/04
CPC分类号： C40B40/02 , A61K39/385 , A61K47/62 , A61K47/645 , A61K47/646 , A61K2039/6031 , C07K7/06 , C07K7/08 , C07K14/005 , C07K2319/00 , C07K2319/735 , C12N15/1037 , C12N2740/16322
摘要： The number of peptides having an ability to bind to a cell or penetrate into a cell is narrowed down by being selectively enriched from a random peptide library with a diversity of not less than one hundred millions of peptides using a phage surface display technique, and then cytoplasmic transfer is evaluated by using protein synthesis inhibition as an indicator by adding to a cell, a fusion body of the selectively enriched peptide and a protein synthesis inhibitory factor (PSIF) that cannot solely penetrate into the cell.
摘要翻译：通过使用噬菌体表面显示技术从具有不少于一亿多种肽的多样性的随机多肽文库中选择性富集具有结合细胞或穿透细胞的能力的肽的数量变窄，然后通过添加细胞，选择性富集的肽的融合体和蛋白质合成抑制因子（PSIF）不能仅仅渗入细胞，通过使用蛋白质合成抑制作为指标来评价细胞质转移。

2. 发明授权

US07989588B2 Cell permeable peptide 有权
标题翻译：细胞可渗透肽
公开(公告)号：US07989588B2
公开(公告)日：2011-08-02
申请号：US11883810
申请日：2006-02-09
申请人： Shinsaku Nakagawa , Tadanori Mayumi , Kiichi Fukui
发明人： Shinsaku Nakagawa , Tadanori Mayumi , Kiichi Fukui
IPC分类号： C07K7/08 , C07K19/00 , A61K47/48
CPC分类号： C40B40/02 , A61K39/385 , A61K47/62 , A61K47/645 , A61K47/646 , A61K2039/6031 , C07K7/06 , C07K7/08 , C07K14/005 , C07K2319/00 , C07K2319/735 , C12N15/1037 , C12N2740/16322
摘要： The number of peptides having an ability to bind to a cell or penetrate into a cell is narrowed down by being selectively enriched from a random peptide library with a diversity of not less than one hundred millions of peptides using a phage surface display technique, and then cytoplasmic transfer is evaluated by using protein synthesis inhibition as an indicator by adding to a cell, a fusion body of the selectively enriched peptide and a protein synthesis inhibitory factor (PSIF) that cannot solely penetrate into the cell.
摘要翻译：通过使用噬菌体表面显示技术从具有不少于一亿多种肽的多样性的随机多肽文库中选择性富集具有结合细胞或穿透细胞的能力的肽的数量变窄，然后通过添加细胞，选择性富集的肽的融合体和蛋白质合成抑制因子（PSIF）不能仅仅渗入细胞，通过使用蛋白质合成抑制作为指标来评价细胞质转移。

3. 发明授权

US07342223B2 Mass spectrometer for biological samples 失效
标题翻译：质谱仪用于生物样品
公开(公告)号：US07342223B2
公开(公告)日：2008-03-11
申请号：US11151466
申请日：2005-06-14
申请人： Kunihiko Ohkubo , Kiichi Fukui , Kazuyoshi Itoh
发明人： Kunihiko Ohkubo , Kiichi Fukui , Kazuyoshi Itoh
IPC分类号： B01D59/44
CPC分类号： H01J49/162 , H01J49/0463
摘要： The mass spectrometer according to the present invention includes a light source for emitting pulse light including a plurality of wavelengths; an ionizer for ionizing molecules of a sample by irradiating the light from the light source to the sample; and a mass analyzer for separating ions ionized in the ionizer according to their mass to charge ratios. For the light source, one including a plurality of ultrashort pulse laser sources each emitting a wavelength different from others, and one emitting ultrashort pulse light including plural wavelengths ranging from the visible region to the infrared region generated by dispersing an ultrashort pulse light with continuous (white) spectrum can be used. Pulse lights having plural wavelengths ranging from near infrared to the ultraviolet region respectively share the role; i.e., one of them vaporizes the sample without fragmenting it, and another ionizes the vaporized sample with the single-photon process or two-photon (or multi-photon) process. This enables ionization of protein complexes as a whole contained in the sample, and enables mass analyses of them.
摘要翻译：根据本发明的质谱仪包括用于发射包括多个波长的脉冲光的光源; 电离器，用于通过将来自光源的光照射到样品上来使样品的分子离子化; 以及质量分析器，用于根据电离质量与电离比分离在离子发生器中离子化的离子。对于光源，包括多个发射不同于其他波长的超短脉冲激光源的光源，以及一个发射包括从可见光区域到红外区域的多个波长的超短脉冲光，通过分散具有连续的超短脉冲光白色）光谱。分别具有从近红外到紫外线区域的多个波长的脉冲光共同作用; 即，其中一个使样品蒸发而不使其分裂，另一个使用单光子过程或双光子（或多光子）过程将蒸发的样品电离。这使得能够将样品中包含的蛋白质复合物电离，并且能够进行质量分析。

4. 发明申请

US20100015638A1 Method for production of antibody directed against cell membrane surface antigen epitope and assaying method 审中-公开
标题翻译：生产针对细胞膜表面抗原表位的抗体的方法和测定方法
公开(公告)号：US20100015638A1
公开(公告)日：2010-01-21
申请号：US11887122
申请日：2006-03-24
申请人： Susumu Uchiyama , Kiichi Fukui
发明人： Susumu Uchiyama , Kiichi Fukui
IPC分类号： G01N33/53
CPC分类号： C07K16/2887 , A61K2039/515 , C07K2317/92
摘要： Disclosed are a method for determining the affinity of an antibody capable of binding to a cell membrane surface antigen for the antigen; and a method for assaying or screening an antibody capable of binding to a cell membrane surface antigen by utilizing the determination method. In the determination of the affinity of the antibody for the antigen, floating cells presenting the antigen on the surface of the cell membrane thereof are used and the B/F separation of the cells is made by centrifugation or on a filter through which the cells cannot pass.
摘要翻译：公开了一种确定能够结合抗原的细胞膜表面抗原的抗体的亲和力的方法; 以及通过利用测定方法测定或筛选能够结合细胞膜表面抗原的抗体的方法。在测定抗体对抗原的亲和性时，使用在其细胞膜表面上呈递抗原的浮游细胞，并通过离心或细胞不能通过的过滤器进行细胞的B / F分离通过。

5. 发明申请

US20130109874A1 POLYNUCLEOTIDE ENCODING PPAT DERIVED FROM JATROPHA AND USE THEREOF 有权
标题翻译：编码从JATROPHA衍生的PPAT的多核苷酸及其用途
公开(公告)号：US20130109874A1
公开(公告)日：2013-05-02
申请号：US13643396
申请日：2011-10-25
申请人： Kiichi Fukui , Joyce Cartagena , Naoki Wada , Tsutomu Kohinata , Yasuhisa Yushio , Naoki Ikeguchi , Satoshi Tabata
发明人： Kiichi Fukui , Joyce Cartagena , Naoki Wada , Tsutomu Kohinata , Yasuhisa Yushio , Naoki Ikeguchi , Satoshi Tabata
IPC分类号： C12N15/82
CPC分类号： C12N15/8247 , C12N9/1241 , C12N15/8243 , C12N15/8271 , C12N15/8273 , C12Y207/07003
摘要： A PPAT polypeptide of SEQ ID NO: 1 derived from Jatropha, a PPAT polynucleotide of SEQ ID NO: 2 and so on were found. By transforming Jatropha with these PPAT polynucleotides, it is possible to overexpress the PPAT polypeptide in comparison with a wild type, and biosynthesis of coenzyme A is promoted by these polypeptides, the metabolic function and viability of the transformed Jatropha are enhanced, and for example, stress resistance can be significantly improved.
摘要翻译：发现衍生自麻疯树的SEQ ID NO：1的PPAT多肽，SEQ ID NO：2的PPAT多核苷酸等。通过用这些PPAT多核苷酸转化麻疯树，可以与野生型相比过表达PPAT多肽，并且这些多肽促进了辅酶A的生物合成，增强了转化的麻疯树的代谢功能和活力，例如，耐应力可显着提高。

6. 发明申请

US20110263825A1 ANTI-CD20 MONOCLONAL ANTIBODIES 审中-公开
标题翻译：抗CD20单克隆抗体
公开(公告)号：US20110263825A1
公开(公告)日：2011-10-27
申请号：US12674783
申请日：2007-09-06
申请人： Susumu Uchiyama , Kiichi Fukui
发明人： Susumu Uchiyama , Kiichi Fukui
IPC分类号： C07K16/28 , C12P21/08
CPC分类号： C07K16/2887 , C07K2317/24 , C07K2317/56 , C07K2317/73 , C07K2317/732 , C07K2317/734 , C07K2317/92
摘要： It is intended to provide a monoclonal antibody having a growth inhibitory activity against a cell having a human CD20 antigen which is produced by using, as immunogens, a human B cell line expressing the human CD20 antigen and a cell line originating in a non-human animal, which is different from an animal to be immunized and has been transformed with human CD20 DNA, and a monoclonal antibody obtained by chimerization or humanization of the above-described monoclonal antibody. These monoclonal antibodies show biological activities suitable for using as drugs.
摘要翻译：旨在提供对具有人CD20抗原的细胞具有生长抑制活性的单克隆抗体，其通过使用表达人CD20抗原的人B细胞系和源自非人类的细胞系作为免疫原产生动物，其与被免疫的动物不同，并且已经用人CD20DNA转化，以及通过上述单克隆抗体的嵌合或人源化获得的单克隆抗体。这些单克隆抗体显示适合用作药物的生物活性。

7. 发明申请

US20090232381A1 Method for Monitoring Cells, System for Cell-Based Assay, and Program for Cell-Based Assay 审中-公开
标题翻译：监测细胞的方法，基于细胞的测定系统和基于细胞的测定的程序
公开(公告)号：US20090232381A1
公开(公告)日：2009-09-17
申请号：US11721120
申请日：2005-10-24
申请人： Sachihiro Matsunaga , Susumu Uchiyama , Kiichi Fukui
发明人： Sachihiro Matsunaga , Susumu Uchiyama , Kiichi Fukui
IPC分类号： G06K9/00
CPC分类号： G01N21/6458 , G01N21/6428 , G01N2021/6423 , G01N2021/6482
摘要： A method for monitoring cells is provided capable of determining the stage in cell cycle of a cell in a rapid and highly reliable manner.A step of obtaining an image that reflects the chromosomal state in the cell, and a step of calculating a parameter that corresponds to the chromosomal state based on the image to determine the stage in the cell cycle on the basis of the calculation result are included.
摘要翻译：提供了一种用于监测细胞的方法，其能够以快速且高度可靠的方式确定细胞的细胞周期的阶段。包括获得反映细胞中的染色体状态的图像的步骤，以及基于该图像计算与染色体状态对应的参数的步骤，以基于计算结果确定细胞周期中的阶段。

8. 发明授权

US07132289B2 Method for introducing foreign matters into living cells 失效
公开(公告)号：US07132289B2
公开(公告)日：2006-11-07
申请号：US10015607
申请日：2001-12-17
申请人： Akio Kobayashi , Kiichi Fukui , Satoshi Harajima , Eiichiro Fukusaki , Shinichiro Kajiyama , Shinya Okuda , Takeshi Shoji
发明人： Akio Kobayashi , Kiichi Fukui , Satoshi Harajima , Eiichiro Fukusaki , Shinichiro Kajiyama , Shinya Okuda , Takeshi Shoji
IPC分类号： C12N15/09 , C12N5/10 , C12N15/63 , C12N15/88 , A01H5/00
CPC分类号： C12N15/87 , C12M35/02 , C12N13/00 , C12N15/8206
摘要： A method for introducing a foreign matter into a cell, includes the steps of placing a small particle carrying a foreign matter at a part of a cell surface of a living cell, boring a hole in a cell wall and/or a cell membrane by irradiating and treating said part of the cell surface with a laser beam, and introducing the foreign matter into the living cell.

9. 发明授权

US09447426B2 Polynucleotide encoding NF-YB derived from jatropha and use thereof 有权
标题翻译：编码来自麻疯树的NF-YB的多核苷酸及其用途
公开(公告)号：US09447426B2
公开(公告)日：2016-09-20
申请号：US13639522
申请日：2011-03-30
申请人： Sachihiro Matsunaga , Tsutomu Kohinata , Kiichi Fukui , Satoshi Tabata
发明人： Sachihiro Matsunaga , Tsutomu Kohinata , Kiichi Fukui , Satoshi Tabata
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8273 , C07K14/415
摘要： By analyzing a Jatropha genome, NF-YB-encoding genes of SEQ ID NOs: 1 to 11, fragments of NF-YB-encoding genes of SEQ ID NOs: 12 and 13, and genes relating thereto were found. By transforming Jatropha with these NF-YB-encoding genes and the like, it is possible to overexpress a NF-YB polypeptide and so on, and to significantly improve the productivity of protein synthesis involved by the NF-YB polypeptide, and to significantly improve the dry stress resistance, for example. As a result, it is possible to create dry stress resistant Jatropha capable of ensuring high growth even under water deficient conditions.
摘要翻译：通过分析麻风树基因组，发现SEQ ID NO：1至11的NF-YB编码基因，SEQ ID NO：12和13的NF-YB编码基因的片段，以及与之相关的基因。通过使用这些NF-YB编码基因等将麻疯树进行转化，可以过表达NF-YB多肽等，并显着提高NF-YB多肽所涉及的蛋白质合成的生产力，并显着提高耐干性，例如。因此，即使在缺水条件下，也可以产生能够确保高生长的耐干旱麻疯树。

10. 发明授权

US09255278B2 Polynucleotide encoding PPAT derived from jatropha and use thereof 有权
标题翻译：编码来自麻疯树的PPAT的多核苷酸及其用途
公开(公告)号：US09255278B2
公开(公告)日：2016-02-09
申请号：US13643396
申请日：2011-10-25
申请人： Kiichi Fukui , Joyce Cartagena , Naoki Wada , Tsutomu Kohinata , Yasuhisa Yushio , Naoki Ikeguchi , Satoshi Tabata
发明人： Kiichi Fukui , Joyce Cartagena , Naoki Wada , Tsutomu Kohinata , Yasuhisa Yushio , Naoki Ikeguchi , Satoshi Tabata
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N9/06 , C12N15/53 , C12N9/12
CPC分类号： C12N15/8247 , C12N9/1241 , C12N15/8243 , C12N15/8271 , C12N15/8273 , C12Y207/07003
摘要： A PPAT polypeptide of SEQ ID NO: 1 derived from Jatropha, a PPAT polynucleotide of SEQ ID NO: 2 and so on were found. By transforming Jatropha with these PPAT polynucleotides, it is possible to overexpress the PPAT polypeptide in comparison with a wild type, and biosynthesis of coenzyme A is promoted by these polypeptides, the metabolic function and viability of the transformed Jatropha are enhanced, and for example, stress resistance can be significantly improved.
摘要翻译：发现衍生自麻疯树的SEQ ID NO：1的PPAT多肽，SEQ ID NO：2的PPAT多核苷酸等。通过用这些PPAT多核苷酸转化麻疯树，可以与野生型相比过表达PPAT多肽，并且这些多肽促进了辅酶A的生物合成，增强了转化的麻疯树的代谢功能和活力，例如，耐应力可显着提高。

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