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1. 发明申请

US20080085512A1 Array assay devices and methods for making and using the same 审中-公开
标题翻译：阵列测定装置及其制造和使用方法
公开(公告)号：US20080085512A1
公开(公告)日：2008-04-10
申请号：US11544395
申请日：2006-10-05
申请人： Petula N. D'Andrade , Eric M. Leproust
发明人： Petula N. D'Andrade , Eric M. Leproust
IPC分类号： C12Q1/68 , G01N33/53 , C12M3/00
CPC分类号： B01L3/508 , B01J2219/00619 , B01J2219/00635 , B01J2219/00637 , B01L2200/0689 , B01L2300/0636 , B01L2300/0822 , B01L2300/161
摘要： Aspects of the invention include methods for producing a backing element for use in an array assay device. An embodiment of the invention includes providing a precursor backing element, e.g., a solid support having a fluid retaining structure on a planar surface thereof, subjecting the precursor backing element to a solvent/plasma treatment step and then contacting the solvent/plasma treated backing element with a surface energy modification agent. Also provided are backing elements produced in accordance with embodiments of the invention, as well as array assay devices that include the backing elements. In addition, methods of using the array assay devices in analyte detection applications, as well as kits for use therein are provided.
摘要翻译：本发明的方面包括用于制备用于阵列测定装置的背衬元件的方法。本发明的一个实施例包括提供前体背衬元件，例如在其平面表面上具有流体保持结构的固体支撑体，对前体背衬元件进行溶剂/等离子体处理步骤，然后使溶剂/等离子体处理的背衬元件具有表面能改性剂。还提供了根据本发明的实施方案生产的背衬元件以及包括背衬元件的阵列测定装置。此外，提供了在分析物检测应用中使用阵列测定装置的方法以及用于其中的试剂盒。

2. 发明授权

US07302348B2 Method and system for quantifying and removing spatial-intensity trends in microarray data 有权
标题翻译：用于量化和消除微阵列数据中的空间强度趋势的方法和系统
公开(公告)号：US07302348B2
公开(公告)日：2007-11-27
申请号：US10859868
申请日：2004-06-02
申请人： Jayati Ghosh , Bill J. Peck , Eric M. Leproust , Charles David Troup , Glenda Choate Delenstarr , Patrick J. Collins , John F. Corson , Paul K. Wolber , Xiangyang Zhou
发明人： Jayati Ghosh , Bill J. Peck , Eric M. Leproust , Charles David Troup , Glenda Choate Delenstarr , Patrick J. Collins , John F. Corson , Paul K. Wolber , Xiangyang Zhou
IPC分类号： G06F19/00 , G06F15/00 , G11C17/00
CPC分类号： G06F19/24 , G06F19/20
摘要： A method and system for quantifying and correcting spatial-intensity trends for each channel of a microarray data set having one or more channels. The method and system of one embodiment of the present invention selects a set of features from each channel of the microarray data set. Based on the selected set of features, a surface is used to determine the intensities for all features in each channel of the microarray data set. Spatial-intensity trends within the microarray data set are quantified, based on the surface to the intensities for each channel of the microarray data set. After the surface has been determined, the spatial-intensity trend can be removed from the microarray data set.
摘要翻译：一种用于量化和校正具有一个或多个通道的微阵列数据集的每个通道的空间强度趋势的方法和系统。本发明的一个实施例的方法和系统从微阵列数据集的每个通道中选择一组特征。基于所选择的特征集合，使用表面来确定微阵列数据集的每个通道中的所有特征的强度。基于微阵列数据集的每个通道的表面到强度，量化微阵列数据集内的空间强度趋势。在确定表面后，可以从微阵列数据集中去除空间强度趋势。

3. 发明授权

US07247337B1 Method and apparatus for microarray fabrication 失效
标题翻译：微阵列制造方法和装置
公开(公告)号：US07247337B1
公开(公告)日：2007-07-24
申请号：US10322280
申请日：2002-12-16
申请人： Eric M. Leproust , Lalitha Reddy , Michel G M Perbost
发明人： Eric M. Leproust , Lalitha Reddy , Michel G M Perbost
IPC分类号： A61L33/00 , B05D3/00
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00378 , B01J2219/00387 , B01J2219/00497 , B01J2219/00527 , B01J2219/00547 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00662 , B01J2219/00675 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731
摘要： The invention provides a method and apparatus for micro array fabrication exclusive from the impact of a gas. A housing with a gas free chamber provides for improved micro array fabrication with a lower defect rate. The invention provides a housing that excludes a gas such as ozone to prevent interaction with probe construction or attachment. A method of fabricating a micro array in an ozone free environment is also disclosed.
摘要翻译：本发明提供一种排除气体影响的微阵列制造方法和装置。具有无气室的壳体提供具有较低缺陷率的改进的微阵列制造。本发明提供一种不包括诸如臭氧的气体以防止与探针结构或附着相互作用的壳体。还公开了一种在无臭氧环境中制造微阵列的方法。

4. 发明授权

US07192710B2 Methods and compositions for high throughput identification of protein/nucleic acid binding pairs 失效
标题翻译：高通量鉴定蛋白质/核酸结合对的方法和组合物
公开(公告)号：US07192710B2
公开(公告)日：2007-03-20
申请号：US10813639
申请日：2004-03-29
申请人： Robert Gellibolian , Eric M. Leproust
发明人： Robert Gellibolian , Eric M. Leproust
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J2219/00378 , B01J2219/00387 , B01J2219/00497 , B01J2219/00527 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00677 , B01J2219/00707 , B01J2219/00722 , B01J2219/00729 , C12Q1/6818 , C40B40/06 , C40B60/14 , C12Q2565/531 , C12Q2561/101
摘要： Methods and compositions for high-throughput identification of protein/nucleic acid binding pairs are provided. In the subject methods, a nucleic acid probe array, e.g., a molecular beacon probe array, is contacted with a target nucleic acid population to produce a hybridized array. The resultant hybridized array is then contacted with a population of proteins to produce a protein bound array. Any resultant array surface bound target nucleic acid/protein complexes are then detected to identify protein/nucleic acid binding pairs. In certain embodiments, the protein and/or nucleic acid members of the identified protein/nucleic acid binding pairs are further characterized. Also provided are systems and kits for use in practicing the subject methods. The subject invention finds use in a variety of different applications.
摘要翻译：提供了用于蛋白质/核酸结合对的高通量鉴定的方法和组合物。在本主题方法中，将核酸探针阵列（例如分子信标探针阵列）与靶核酸群体接触以产生杂交阵列。然后将所得杂交阵列与蛋白质群体接触以产生蛋白质结合阵列。然后检测任何得到的阵列表面结合的靶核酸/蛋白质复合物，以鉴定蛋白质/核酸结合对。在某些实施方案中，进一步表征鉴定的蛋白质/核酸结合对的蛋白质和/或核酸成员。还提供了用于实践主题方法的系统和工具包。本发明可用于各种不同的应用。

5. 发明申请

US20070021919A1 Silico design of chemical arrays 审中-公开
标题翻译：化学阵列的硅胶设计
公开(公告)号：US20070021919A1
公开(公告)日：2007-01-25
申请号：US11185207
申请日：2005-07-19
申请人： Eric M. Leproust , Michael G. Booth
发明人： Eric M. Leproust , Michael G. Booth
IPC分类号： G06F19/00
CPC分类号： G16B25/00 , G16B50/00
摘要： A system is described which comprises an input manager for receiving probe request information from a probe requester and a processing module configured to identify a probe sequence associated with the probe request information, wherein the processing module associates a flag with one or more nucleic acid sequences corresponding to the probe request associated with a predefined sequence characteristic. Methods of using the system and computer program products for implementing the methods are also described.
摘要翻译：描述了一种系统，其包括用于从探测请求者接收探测请求信息的输入管理器和被配置为识别与探测请求信息相关联的探测序列的处理模块，其中所述处理模块将标记与相应的一个或多个核酸序列相关联到与预定义的序列特征相关联的探测请求。还描述了使用系统和计算机程序产品来实现该方法的方法。

6. 发明授权

US07078505B2 Manufacture of arrays with varying deposition parameters 失效
标题翻译：制造具有不同沉积参数的阵列
公开(公告)号：US07078505B2
公开(公告)日：2006-07-18
申请号：US10165783
申请日：2002-06-06
申请人： Jay K. Bass , Maryam Mobed-Miremadi , Michelle M. Maranowski , Roy H. Kanemoto , Bill J. Peck , Eric M. Leproust , Michael P. Caren
发明人： Jay K. Bass , Maryam Mobed-Miremadi , Michelle M. Maranowski , Roy H. Kanemoto , Bill J. Peck , Eric M. Leproust , Michael P. Caren
IPC分类号： C07H21/04 , C12Q1/68 , C12M1/36
CPC分类号： C03C17/32 , B01J2219/00286 , B01J2219/00378 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00637 , B01J2219/00689 , B01J2219/00695 , B01J2219/00698 , B01J2219/00722 , B01L3/0241 , C03C17/002 , C40B40/06 , C40B60/14
摘要： Methods are disclosed for synthesizing a plurality of compounds on the surface of supports. The synthesis comprises a series of cycles of steps in which reagents for conducting the synthesis are deposited on the surface of the support to form the chemical compounds. At least one physical parameter of the deposition varies between cycles The method comprises conducting the synthesis in at least two sets of cycles, arbitrarily designated as a first set and a second set. Each cycle comprises at least one step of depositing reagents on a surface by means of droplet dispensing elements that traverse the surface of the support. At least one, and desirably all, of the following deposition parameters are employed as indicated: The number of times of repetition for a step of depositing reagents in at least one cycle of the second set is less than the number of times of repetition for a corresponding step in at least one cycle of the first set. The rate of deposition in at least one cycle of the second set is slower than or equal to the rate of deposition in at least one cycle of the first set. The rate of traversal of the dispensing elements over the surface of the support in at least one cycle of the second set is greater than such rate of traversal in at least one cycle of the first set.
摘要翻译：公开了在载体表面上合成多种化合物的方法。该合成包括一系列循环的步骤，其中用于进行合成的试剂沉积在载体的表面上以形成化合物。沉积物的至少一个物理参数在循环之间变化。该方法包括以任意指定为第一组和第二组的至少两组循环进行合成。每个循环包括至少一个步骤，其通过穿过载体表面的液滴分配元件在表面上沉积试剂。如下所示使用以下沉积参数中的至少一个，期望地全部以下沉积参数：在第二组的至少一个循环中沉积试剂的步骤的重复次数小于第二组的重复次数在第一组的至少一个循环中的对应步骤。在第二组的至少一个循环中的沉积速率慢于或等于第一组的至少一个循环中的沉积速率。在第二组的至少一个循环中，分配元件在支撑体表面上的遍历速率大于在第一组的至少一个循环中的穿过速率。

7. 发明授权

US08415138B2 Apparatuses and methods for oligonucleotide preparation 有权
标题翻译：寡核苷酸制备的设备和方法
公开(公告)号：US08415138B2
公开(公告)日：2013-04-09
申请号：US11469405
申请日：2006-08-31
申请人： Eric M. Leproust
发明人： Eric M. Leproust
IPC分类号： C12M1/00 , C12Q1/68 , C07H19/00 , C07H21/00 , B01J10/00 , B01J19/00 , B01J19/18
CPC分类号： C07H21/00
摘要： Provided are improved processes and apparatuses for solid phase oligonucleotide synthesis, the improvements comprising carrying out detritylation of the nascent oligonucleotide using a composition of an organic solvent, a protic solvent and a selected concentration, or selected concentration range, of water. In some embodiments, an oligonucleotide synthesis apparatus includes means for adding water to a detritylation reagent. In some embodiments, an apparatus can include a water detector for analyzing the water concentration of a detritylation reagent to be reacted with a nascent oligonucleotide. An apparatus can comprise a feedback loop to control the concentration of water at the point of the detritylation reaction and/or to control the detritylation reaction time. The apparatuses and methods reduce batch-to-batch variations in the manufacture of oligonucleotides immobilized on the surface of various substrates.
摘要翻译：提供了用于固相寡核苷酸合成的改进的方法和装置，其改进包括使用有机溶剂，质子溶剂和所选浓度或选定浓度范围的水进行新生寡核苷酸的去结合。在一些实施方案中，寡核苷酸合成装置包括向去离子化试剂中加入水的装置。在一些实施例中，装置可以包括用于分析与新生寡核苷酸反应的去离子试剂的水浓度的水检测器。装置可以包括反馈回路以控制在去离子化反应的点处的水的浓度和/或控制不稳定反应时间。这些装置和方法减少了固定在各种底物表面上的寡核苷酸制造中的批次间变化。

8. 发明申请

US20110230371A1 Chemical Arrays and Methods of Using the Same 有权
标题翻译：化学数组及其使用方法
公开(公告)号：US20110230371A1
公开(公告)日：2011-09-22
申请号：US13116998
申请日：2011-05-26
申请人： Mel N. Kronick , Eric M. Leproust
发明人： Mel N. Kronick , Eric M. Leproust
IPC分类号： C40B40/04 , C40B50/14
CPC分类号： C40B30/04 , G01N33/54366
摘要： Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.
摘要翻译：提供了从初始化学阵列产生产物分子的混合物的方法和组合物。在本发明方法中，表面固定的第一部分的化学阵列经受切割条件，使得产生溶液相第一部分的组合物。然后将所得到的溶液相第一部分的组合物与一种或多种反应物接触以产生不同于第一部分的产物分子的混合物。还提供了用于实践本发明方法的主题方法和试剂盒中使用的阵列。

9. 发明授权

US07534561B2 Nucleic acid array in situ fabrication methods and arrays produced using the same 有权
标题翻译：核酸阵列原位制备方法和使用其制备的阵列
公开(公告)号：US07534561B2
公开(公告)日：2009-05-19
申请号：US10407080
申请日：2003-04-02
申请人： Theodore R. Sana , Eric M. Leproust , Michel G. M. Perbost , Paul K. Wolber
发明人： Theodore R. Sana , Eric M. Leproust , Michel G. M. Perbost , Paul K. Wolber
IPC分类号： C12Q1/68 , C12N11/16 , C07H21/04 , C12P19/34
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/0036 , B01J2219/00378 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00693 , B01J2219/00695 , B01J2219/00707 , B01J2219/00722 , C12Q1/6837 , C40B40/06 , C40B50/14 , C40B60/14 , C12Q2565/513 , C12Q2545/101
摘要： Methods and devices for producing a nucleic acid arrays using in situ nucleic acid array synthesis protocols are provided. A feature of certain embodiments of the invention is that control probes are produced in collections of features, e.g., columns, of the arrays that have been selected according to a particular efficient control probe feature/column selection protocol. A feature of certain other embodiments of the invention is that an “all-bases-all-layers” probe set is produced in at least one of column of the arrays. Also provided are devices configured for use in the subject methods, as well as arrays produced using the subject methods and devices as well as methods for using such arrays.
摘要翻译：提供了使用原位核酸阵列合成方案制备核酸阵列的方法和装置。本发明的某些实施例的特征在于，控制探针是根据特定的有效控制探针特征/列选择协议选择的阵列的特征（例如列）的集合产生的。本发明的某些其他实施例的特征在于，在全部阵列中的至少一列中产生“全基底全层”探针组。还提供了被配置为在主题方法中使用的装置以及使用主题方法和装置产生的阵列以及使用这些阵列的方法。

10. 发明申请

US20080207960A1 Methods, compositions, and kits for post-hybridization processing of arrays 审中-公开
标题翻译：用于阵列后杂交处理的方法，组合物和试剂盒
公开(公告)号：US20080207960A1
公开(公告)日：2008-08-28
申请号：US11713377
申请日：2007-02-28
申请人： Eric Lin , Eric M. Leproust , Patrick J. Collins
发明人： Eric Lin , Eric M. Leproust , Patrick J. Collins
IPC分类号： C07H21/04 , C07C19/08 , C07F7/02
CPC分类号： C07H21/04 , C07C1/26 , C07C17/10 , C07C2529/40 , C07C9/00 , C07C19/075
摘要： In some embodiments, the present disclosure relates to systems, compositions, and methods for treating a microarray. The compositions include a superwetting agent. The methods include contacting the microarray with an aqueous mixture including the superwetting agent after a hybridization step. Kits for carrying out the methods are also provided.
摘要翻译：在一些实施方案中，本公开涉及用于治疗微阵列的系统，组合物和方法。组合物包括超润湿剂。所述方法包括在杂交步骤之后使微阵列与包含超润湿剂的含水混合物接触。还提供了实施方法的工具包。

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