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1. 发明申请

WO2002071834A2 BIOTIN-BINDING COMPOUNDS FOR INDUCTION OF STERILITY IN PLANTS 审中-公开
标题翻译：用于诱导植物不育的生物结合化合物
公开(公告)号：WO2002071834A2
公开(公告)日：2002-09-19
申请号：PCT/US2002/006554
申请日：2002-03-06
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , ALBERTSEN, Marc , HUFFMAN, Gary
发明人： ALBERTSEN, Marc , HUFFMAN, Gary
IPC分类号： A01H
CPC分类号： C12N9/93 , C12N15/8289 , C12N15/829
摘要： Sterile plants can be produced by increasing the endogenous concentration of a biotin-binding protein in the plant tissues, preferably those tissues that are critical to gamete formation or function. This effect can be achieved by producing transgenic plants containing an expression vector in which a promoter is operably linked to a DNA sequence encoding a biotin-binding polypeptide. Preferred biotin-binding proteins have a low susceptibility to degradation, are readily digested, or have a low allergenic potential. Other preferred polypeptides are not highly detrimental to cellular viability, such that low level expression in cells other than those critical for gamete formation or function may be tolerated more easily. This allows the use of promoters having low level expression in non-targeted tissues, thus expanding the repertoire of promoters useful for affecting sterility. Methods for restoring fertility are disclosed. Additionally, methods for production of seeds with one or more desired grain traits are provided.
摘要翻译：可以通过增加植物组织中生物素结合蛋白的内源性浓度，优选那些对配子形成或功能至关重要的那些组织来产生无菌植物。可以通过产生含有表达载体的转基因植物来实现该效果，其中启动子可操作地连接到编码生物素结合多肽的DNA序列。优选的生物素结合蛋白对降解的敏感性低，容易消化或具有低变态潜能。其它优选的多肽对细胞活力没有高度的不利影响，因此可以更容易地容忍对细胞以外的细胞的低水平表达，而不是那些对配子形成或功能至关重要的细胞。这允许使用在非靶组织中具有低水平表达的启动子，从而扩大可用于影响不育的启动子的所有组成成分。公开了恢复生育能力的方法。此外，提供了具有一种或多种所需颗粒性状的种子生产方法。

2. 发明申请

WO2002063021A2 NUCLEOTIDE SEQUENCE MEDIATING MALE FERTILITY AND METHOD OF USING SAME 审中-公开
标题翻译：核苷酸序列传播雄性生殖力及其使用方法
公开(公告)号：WO2002063021A2
公开(公告)日：2002-08-15
申请号：PCT/US2002/002713
申请日：2002-01-30
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ALBERTSEN, Marc , FOX, Timothy , HUFFMAN, Gary , TRIMNELL, Mary
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N15/8231 , C12N15/8289
摘要： Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants.
摘要翻译：描述了在植物中介导雄性能育性的核苷酸序列，其中阐述了DNA分子和氨基酸序列。启动子序列及其基本区域也被确定。核苷酸序列可用于介导植物中的雄性能育性。

3. 发明公开

EP1358335A2 NUCLEOTIDE SEQUENCE MEDIATING MALE FERTILITY AND METHOD OF USING SAME 有权
标题翻译：男性生育中介核苷酸及其用途
公开(公告)号：EP1358335A2
公开(公告)日：2003-11-05
申请号：EP02720868.5
申请日：2002-01-30
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ALBERTSEN, Marc , FOX, Timothy , HUFFMAN, Gary , TRIMNELL, Mary
IPC分类号： C12N15/53
CPC分类号： C07K14/415 , C12N15/8231 , C12N15/8289
摘要： Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants.

4. 发明公开

EP1255825A2 MALE TISSUE-SPECIFIC REGULATORY REGION AND METHOD OF USING SAME 审中-公开
标题翻译：雄性组织特异性调节区和它们的使用方法
公开(公告)号：EP1255825A2
公开(公告)日：2002-11-13
申请号：EP01910583.2
申请日：2001-02-13
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ALBERTSEN, Marc, C. , FOX, Timothy, W. , GARNAAT, Carl, W. , HUFFMAN, Gary , KENDALL, Timmy, L.
IPC分类号： C12N15/09 , C12N15/82
CPC分类号： C07K14/415 , C12N15/8231 , C12N15/8289
摘要： The present invention relates to an isolated nucleic acid sequence encoding the Ms45 male tissue-preferred regulatory region. In one aspect this invention relates to the use of this male tissue-preferred regulatory region in mediating fertility. An example of such use is the production of hybrid seed such as in a male sterility system. The Ms45 male tissue-preferred regulatory region can be operably linked with exogenous genes, such as those encoding cytotoxins, complementary nucleotidic units and inhibitory molecules. This invention also relates to plant cells, plant tissue and differentiated plants which contain the regulatory region in this invention.

5. 发明授权

EP1358335B1 NUCLEOTIDE SEQUENCE MEDIATING MALE FERTILITY AND METHOD OF USING SAME 有权
标题翻译：男性生育中介核苷酸及其用途
公开(公告)号：EP1358335B1
公开(公告)日：2011-04-13
申请号：EP02720868.5
申请日：2002-01-30
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ALBERTSEN, Marc , FOX, Timothy , HUFFMAN, Gary , TRIMNELL, Mary
IPC分类号： C12N15/53
CPC分类号： C07K14/415 , C12N15/8231 , C12N15/8289
摘要： Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants.

6. 发明授权

EP1320618B1 NUCLEOTIDE SEQUENCES MEDIATING MALE FERTILITY AND METHOD OF USING SAME 有权
标题翻译：男性生育中介核苷酸及其用途
公开(公告)号：EP1320618B1
公开(公告)日：2012-03-07
申请号：EP01971332.0
申请日：2001-09-25
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ALBERTSEN, Marc, C. , FOX, Timothy , HUFFMAN, Gary , TRIMNELL, Mary
IPC分类号： C12N15/82 , C12N15/29 , A01H5/00
CPC分类号： C12N15/8289 , C07K14/415 , C12N15/8217 , C12N15/8222 , C12N15/8231
摘要： Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants.

7. 发明公开

EP1320618A2 NUCLEOTIDE SEQUENCES MEDIATING MALE FERTILITY AND METHOD OF USING SAME 有权
标题翻译：男性生育中介核苷酸及其用途
公开(公告)号：EP1320618A2
公开(公告)日：2003-06-25
申请号：EP01971332.0
申请日：2001-09-25
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ALBERTSEN, Marc, C. , FOX, Timothy , HUFFMAN, Gary , TRIMNELL, Mary
IPC分类号： C12N15/82 , C12N15/29 , A01H5/00
CPC分类号： C12N15/8289 , C07K14/415 , C12N15/8217 , C12N15/8222 , C12N15/8231
摘要： Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants.

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