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1. 发明申请

US20210002696A1 METHOD FOR AMPLIFYING TARGET NUCLEIC ACID AND COMPOSITION FOR AMPLIFYING TARGET NUCLEIC ACID 有权
公开(公告)号：US20210002696A1
公开(公告)日：2021-01-07
申请号：US16768356
申请日：2018-11-28
申请人： PANAGENE INC.
发明人： Jaejin Park , Minseo Kim
IPC分类号： C12Q1/686 , C12Q1/6848
摘要： The present invention relates to a method for detecting a target nucleic acid, which induces any surrogate target to be amplified in the presence of the target nucleic acid, and more particularly, to a method of implementing target nucleic acid detection with high efficiency and sensitivity by inducing production of a fusion amplicon between the target nucleic acid and any surrogate target when the target nucleic acid is present, and amplifying the fusion amplicon, and a polymerase chain reaction (PCR) composition for implementing the method. According to the target nucleic acid detection method of the present invention, a portion of the nucleotide sequence of the polymerase chain reaction (PCR) product may be arbitrarily adjusted, and consequently, dependence on the nucleotide sequence of the target nucleic acid may be minimized. Thus, even if detection of the region of interest of the target nucleic acid is difficult due to the excessively high G/C content of the region, detection thereof may be effectively performed. In addition, since an arbitrary nucleotide sequence may be inserted between thetarget nucleic acid-complementary nucleotide sequence portion and the amplification primer-complementary nucleotide sequence of the surrogate target, a universal probe that hybridizes with this arbitrary nucleotide sequence may be prepared. Thus, even though the nucleotide sequence of the target nucleic acid is6 changed, the universal probe can advantageously be used continuously. In addition, according to the target nucleic acid detection method of the present invention, only the sensitivity of target nucleic acid amplification can be independently adjusted by controlling the amount of surrogate target added, and at the same time, it is possible to minimize the fluctuation of detection efficiency in the optimization of sensitivity in the development of diagnostic product,thus it is useful for molecular diagnosis, prenatal diagnosis, early diagnosis, cancer diagnosis, genetic related diagnosis, genetic trait diagnosis, diagnosis of infectious bacteria, identification of drug-resistant bacteria, forensic medicine, species identification of organisms, and the like.

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