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1. 发明专利

JPH0716100A DETERMINATION OF SERUM IRON AND COMPOSITION THEREFOR 失效
公开(公告)号：JPH0716100A
公开(公告)日：1995-01-20
申请号：JP17373092
申请日：1992-06-09
申请人： ORIENTAL YEAST CO LTD
发明人： HAMAZAKI HOZUMI , NONOBE MASATSUGU , FUJITA TAKESHI
IPC分类号： C12Q1/32 , C12Q1/527
摘要： PURPOSE:To easily and quickly determine serum iron accurately without the need for any skill by quantifying serum iron using an aconitase with iron serving as an activating factor. CONSTITUTION:Serum iron bound to transferrin is liberated by an acidic solution and added to a buffer solution where apo-type aconitase has been dissolved, forming holo-type aconitase. The aimed serum iron level is determined from aconitase activity altered depending on the serum iron level using the level of isocitric acid formed by the reaction of the aconitase as representing the changes from NADs and/or NANPs to NADH and/or NADPHs by isocitric acid dehydrogenase. With this method, low-level serum iron can be determined in high sensitivity.

2. 发明专利

JPH0697A DETERMINATION OF UNSATURATED IRON BINDING ABILITY AND REAGENT THEREFOR 失效
公开(公告)号：JPH0697A
公开(公告)日：1994-01-11
申请号：JP18463492
申请日：1992-06-19
申请人： ORIENTAL YEAST CO LTD
发明人： HAMAZAKI HOZUMI , NONOBE MASATSUGU , FUJITA TAKESHI
IPC分类号： C12Q1/32 , C12Q1/527
摘要： PURPOSE:To quickly, easily determine unsaturated iron binding ability in high sensitivity by adding an excess of iron to a specimen to bind the iron to transferrin, binding the free residual iron to aconitase and by measuring the resultant activated aconitase. CONSTITUTION:An excess of iron at a known concentration is added to a specimen such as humor to bind the iron to transferrin present in the specimen in the presence of a surfactant; thence, the free residual iron is brought into contact with apo-type aconitase inert unless iron is added to convert such aconitase to active-type aconitase. The isocitric acid produced from citric acid through being catalyzed by this aconitase is then detected using isocitric acid dehydrogenase as detective enzyme and nicotinamidoadeninedinucleotide and/or nicotinamidoadeninedinucleotide phosphate as coenzyme for the above dehydrogenase, thus accomplishing the objective quick, easy determination of the unsaturated iron binding ability for e.g. humor without the need for any skill.

3. 发明专利

JPH06153993A METHOD FOR DETERMINING SERUM IRON AND DETERMINING COMPOSITION 失效
公开(公告)号：JPH06153993A
公开(公告)日：1994-06-03
申请号：JP34092492
申请日：1992-11-30
申请人： ORIENTAL YEAST CO LTD
发明人： HAMAZAKI HOZUMI , NONOBE MASATSUGU , FUJITA TAKESHI
IPC分类号： C12Q1/32 , C12Q1/527
摘要： PURPOSE:To accurately and efficiently determine serum iron even in a very small amount in simple operation without any measuring mistake by isolating iron from transferrin in serine and measuring aconitase activity while binding the isolated iron to aconitase. CONSTITUTION:A buffer solution of acidic pH is added to serum to isolate iron from transferase in serum and this isolated iron is bound to aconitase by action of a reducing agent such as ascorbic acid on the isolated iron to form an active type aconitase. Sodium citrate is converted into isocitric acid by this active type aconitase and this isocitric acid is made to react with isocitric acid dehydrogenase using nicotinamide adenine dinucleotide phosphates(NAD) or/and nicotinamide adenine dinucleotide phosphates(NADP) as coenzymes and the serum iron is accurately and effectively determined even in very small amount from amounts of these coenzymes converted to reduction type by measurement of 340nm absorption due to spectrophotometer.

4. 发明专利

JPH05111396A METHOD FOR MEASURING ACTIVITY OF CREATINE KINASE AND REAGENT USED THEREFOR 失效
公开(公告)号：JPH05111396A
公开(公告)日：1993-05-07
申请号：JP20108191
申请日：1991-07-17
申请人： ORIENTAL YEAST CO LTD
发明人： NONOBE MASATSUGU , HAMAZAKI HOZUMI , FUJITA TAKESHI , SUGITA KAZUJI , KITAHARA TETSUO , FURUKATA CHIZURU
IPC分类号： C12Q1/32 , C12Q1/48 , C12Q1/50 , C12Q1/54
摘要： PURPOSE:To accurately and efficiently measure the subject activity for diagnosing cardiac infarction, etc., by using a reaction system containing creatine phosphate, ADP, hexokinase, etc., glucose, glucose 6-phosphate dehydrogenase and thio-NAD, etc. CONSTITUTION:A reactional system containing at least creatine phosphate, adenosine diphosphate (ADP), hexokinase or glucokinase, glucose, glucose 6- phosphate dehydrogenase and thionicotinamide adenine dinucleotide (thio-NAD) or thionicotinamide adenine dinucleotide phosphate (thio-NADP) and further ethylenediaminetetraacetic acid (EDTA), adenosine monophosphate (AMP), P , P -diadenosine-5'-pentaphosphate and/or a buffer optimum within the range of pH 6-7 is brought into contact with a test sample to detect a detectable change in absorbance, etc., of a coenzyme. Thereby, the activity of creatine kinase is accurately and efficiently measured.

5. 发明专利

JPH0565292A CRYSTALLINE THIONICOTINAMIDE-ADENINE DINUCLEOTIDE PHOSPHORIC ACID POTASSIUM SALT AND ITS PRODUCTION 失效
公开(公告)号：JPH0565292A
公开(公告)日：1993-03-19
申请号：JP12318291
申请日：1991-03-05
申请人： ORIENTAL YEAST CO LTD
发明人： SUGITA KAZUJI , KITAHARA TETSUO , NONOBE MASATSUGU , FUJITA TAKESHI
IPC分类号： C07B63/00 , C07H19/207
摘要： PURPOSE:To provide the subject new compound having no hygroscopicity and exhibiting no deliquescence even in contact with air and, accordingly, preservable over a long period, having high purity and high stability, absolutely free from inhibiting agent and useful as an investigation reagent, diagnostic agent, etc. CONSTITUTION:Crystalline thionicotinamide-adenine dinucleotide phosphoric acid potassium salt (crystalline thio-NADP.K salt). The compound can be produced by adding a hydrophilic organic solvent (preferably methanol) to an aqueous solution of thio-NADP.K salt and separating the precipitated crystal. Preferably, 0.3-1 pt. of methanol is added to 1pt. of a 10-20% aqueous solution of thio-NADP.K salt at pH2 and the mixture is maintained at room temperature until the crystallization takes place, cooled to 0-4 deg.C and left standing for 1-30hr.

6. 发明专利

JP2014135930A Method for imparting polyphosphoric acid availability to atp-dependent kinase 有权
标题翻译：将聚磷酸可用性转移到ATP依赖性激酶的方法
公开(公告)号：JP2014135930A
公开(公告)日：2014-07-28
申请号：JP2013006341
申请日：2013-01-17
申请人： Kyoto Univ , 国立大学法人京都大学 , Oriental Yeast Co Ltd , オリエンタル酵母工業株式会社
发明人： MURATA KOSAKU , KAWAI SHIGEYUKI , NAKAMICHI YUSUKE , UCHIDA KOJI , NONOBE MASATSUGU
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/12 , C12P19/32
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide means for imparting polyphosphoric acid availability to ATP-dependent kinase.SOLUTION: There is provided a method for imparting a polyphosphoric acid availability to an ATP-dependent kinase, which includes substituting a Thr residue for an Asn residue of a GGDGN motif in an ATP-dependent kinase consisting of an amino acid sequence including the GGDGN motif.
摘要翻译：要解决的问题：提供赋予ATP依赖性激酶多磷酸可用性的方法。解决方案：提供了赋予ATP依赖性激酶多磷酸可用性的方法，其包括用Thr残基取代Asn残基的由包含GGDGN基序的氨基酸序列组成的ATP依赖性激酶中的GGDGN基序。

7. 发明专利

JP2008054606A Highly biotin-containing yeast and method for producing the same 有权
标题翻译：高生物含量的YEAST及其生产方法
公开(公告)号：JP2008054606A
公开(公告)日：2008-03-13
申请号：JP2006236886
申请日：2006-08-31
申请人： Oriental Yeast Co Ltd , オリエンタル酵母工業株式会社
发明人： NONOBE MASATSUGU , MASUDA YOSHIFUMI , OKA OSAMU
IPC分类号： C12N1/16 , A23L1/28
摘要： PROBLEM TO BE SOLVED: To provide a highly biotin-containing yeast which contains biotin absorbed in high concentration and little has the odor of the yeast, and to provide a method for producing the same, especially a method for producing the highly biotin-containing yeast, comprising finding culture conditions for inhibiting the proliferation of yeast and simultaneously remarkably absorbing the biotin, and then culturing the yeast under the conditions. SOLUTION: This method for producing the highly biotin-containing yeast is characterized by dissolving biotin in a solvent, preferably an alkaline solvent, adding the solution to a carbon source-free yeast culture solution, adjusting the pH of the yeast culture solution on the acidic side, and then culturing the yeast culture solution at a high temperature. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：为了提供高生物素的酵母，其含有高浓度吸收的生物素，并且几乎没有酵母的气味，并且提供其生产方法，特别是生产高生物素的方法包括发现用于抑制酵母增殖并同时显着吸收生物素的培养条件，然后在条件下培养酵母。解决方案：用于生产高生物素的酵母的方法的特征在于将生物素溶解在溶剂中，优选碱性溶剂中，将该溶液加入到不含碳源的酵母培养液中，调节酵母培养液的pH 在酸性方面，然后在高温下培养酵母培养液。版权所有（C）2008，JPO＆INPIT

8. 发明专利

JPH06209791A METHD FOR MEASURING COPPER 失效
公开(公告)号：JPH06209791A
公开(公告)日：1994-08-02
申请号：JP1939793
申请日：1993-01-13
申请人： ORIENTAL YEAST CO LTD
发明人： NONOBE MASATSUGU , HAMAZAKI HOZUMI , FUJITA TAKESHI
IPC分类号： C12Q1/26
摘要： PURPOSE:To accurately, simply and rapidly measure the amount of copper in the living body by determining the copper with a galactose oxidase. CONSTITUTION:A galactose oxidase, preferably holo type galactose oxidase is treated to produce an apo type galactose oxidase, which is then added to a biological sample to measure a change in activit of the apo type galactose oxidase by a change in copper concentration.

9. 发明专利

JPH05219992A METHOD FOR DETERMINING CALCIUM 失效
公开(公告)号：JPH05219992A
公开(公告)日：1993-08-31
申请号：JP5604492
申请日：1992-02-07
申请人： ORIENTAL YEAST CO LTD
发明人： NONOBE MASATSUGU , HAMAZAKI HOZUMI , FUJITA TAKESHI
IPC分类号： C12Q1/48
摘要： PURPOSE:To rapidly and accurately determine Ca, etc., of body fluid without deprotenizing by bringing Ca in a specimen into contact with transglutaminase requiring Ca as an activated factor and measuring activity of enzyme changing depending upon the amount of Ca in the specimen. CONSTITUTION:Calcium in body fluid, especially blood in a specimen is brought into contact with transglutaminase requiring Ca as an activated factor and activity of transglutaminase changing depending upon the amount of Ca in the specimen is accurately determined by measuring change in a donor of the formula Z-L-Gin-Gly (Z is benzyloxycarbonyl), Z-L-Gln, Boc-L-Gln (Boc is t- butyloxycarbonyl), Fmoc-L-Gln (Fmoc is 9-fluorenylmethoxycarbonyl) and/or a receptor such as n-propylamine, n-butylamine or n-amylamine as a substrate of transglutaminase without deprotenizing.

10. 发明专利

JPH05154000A METHOD FOR DETERMINING IRON 失效
公开(公告)号：JPH05154000A
公开(公告)日：1993-06-22
申请号：JP34180891
申请日：1991-12-02
申请人： ORIENTAL YEAST CO LTD
发明人： HAMAZAKI HOZUMI , NONOBE MASATSUGU , FUJITA TAKESHI
IPC分类号： C12Q1/32 , C12Q1/527
摘要： PURPOSE:To accurately and effectively measure even a very small amount of iron by using aconitase requiring iron as an activating factor. CONSTITUTION:Aconitase is blended with a reducing agent (e.g. ascorbic acid or thioglycolic acid), citric acid, isocitric dehydrogenase, nicotinamide adenine dinucleotide or nicotinamide adenine dinucleatide phosphate and a buffer solution to give a reagent, which is used to carry out the objective determination.

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