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    • 8. 发明专利
    • ENZYMATIC REACTION
    • JPH02245190A
    • 1990-09-28
    • JP6819489
    • 1989-03-20
    • NITTO DENKO CORP
    • ITO MASAAKISAIGA TAKESHISAHASHI HIROKOYOSHII RIKAKOISHIZUKA HIROTOSHI
    • C12P1/00C12N11/00
    • PURPOSE:To reduce decrease in flow velocity and to carry out longterm enzymatic reaction by bringing a substrate solution into contact with a porous layer side of an enzyme immobilized filter comprising a dense layer and a porous layer to which an enzyme is immobilized and circulating and feeding the substrate solution at a specific linear velocity of filter face. CONSTITUTION:In carrying out enzymatic reaction using an enzyme immobilized filter prepared by immobilizing an enzyme to a porous layer of an anisotropic ultrafilter wherein a dense layer is integrated with the porous layer, a substrate solution is brought into contact with the porous layer side of the enzyme immobilized film, circulated and fed at 1-5m/second average linear velocity of of film face, enzymatic reaction is carried out and the reaction product is taken out to the dense layer side. By setting the average linear velocity of film face in this range, natural counter current of filtrate caused by pressure loss between a circulation inlet side and outlet side of substrate and forced washing of counter current is eliminated.