专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US09758780B2 Whole genome mapping by DNA sequencing with linked-paired-end library 有权
公开(公告)号：US09758780B2
公开(公告)日：2017-09-12
申请号：US14728223
申请日：2015-06-02
申请人： Ming Xiao , Justin Sibert
发明人： Ming Xiao , Justin Sibert
IPC分类号： C12Q1/68 , C12N15/10 , C07H21/02 , G06F19/18
CPC分类号： C12N15/1068 , C12Q1/6806 , G06F19/18
摘要： The present invention relates to innovative means of generating sequence-linked DNA fragments and subsequent uses of such linked DNA fragments for de novo haplotype-resolved whole genome mapping and massively parallel sequencing. In various embodiments described herein, the methods of the invention relate to methods of generating paired-end nucleic acid fragment sharing common linker nucleic acid sequences using a nicking endonuclease, a T7 endonuclease, a restriction enzyme, or a transposase, methods of analyzing the nucleotides sequences from the linked-paired-end sequenced fragments, and methods of de novo whole genome mapping. Thus, the methods of this invention allow establishing sequence contiguity across the whole genome, and achieving high-quality, low-cost de novo assembly of complex genomes.

2. 发明申请

US20150344873A1 Whole Genome Mapping by DNA Sequencing With Linked-Paired-End Library 有权
标题翻译：通过DNA测序的全基因组映射与链接配对文库
公开(公告)号：US20150344873A1
公开(公告)日：2015-12-03
申请号：US14728223
申请日：2015-06-02
申请人： Ming Xiao , Justin Sibert
发明人： Ming Xiao , Justin Sibert
IPC分类号： C12N15/10 , G06F19/18 , C12Q1/68
CPC分类号： C12N15/1068 , C12Q1/6806 , G06F19/18
摘要： The present invention relates to innovative means of generating sequence-linked DNA fragments and subsequent uses of such linked DNA fragments for de novo haplotype-resolved whole genome mapping and massively parallel sequencing. In various embodiments described herein, the methods of the invention relate to methods of generating paired-end nucleic acid fragment sharing common linker nucleic acid sequences using a nicking endonuclease, a T7 endonuclease, a restriction enzyme, or a transposase, methods of analyzing the nucleotides sequences from the linked-paired-end sequenced fragments, and methods of de novo whole genome mapping. Thus, the methods of this invention allow establishing sequence contiguity across the whole genome, and achieving high-quality, low-cost de novo assembly of complex genomes.
摘要翻译：本发明涉及产生序列连接的DNA片段的创新方法以及随后使用这种连接的DNA片段进行从头单体型解析的全基因组测绘和大规模并行测序。在本文描述的各种实施方案中，本发明的方法涉及使用切口内切核酸酶，T7内切核酸酶，限制酶或转座酶产生分支共同连接体核酸序列的配对末端核酸片段的方法，分析核苷酸的方法来自连锁配对末端测序片段的序列，以及从头整体基因组测绘的方法。因此，本发明的方法允许跨整个基因组建立序列连续性，并实现复杂基因组的高品质，低成本的从头装配。

3. 发明申请

US20110171634A1 METHODS AND DEVICES FOR SINGLE-MOLECULE WHOLE GENOME ANALYSIS 有权
标题翻译：用于单分子全基因组分析的方法和设备
公开(公告)号：US20110171634A1
公开(公告)日：2011-07-14
申请号：US13001697
申请日：2009-06-30
申请人： Ming Xiao , Parikshit A. Deshpande , Han Cao , Michael Austin , Kandaswamy Vijayan , Alexey Y. Sharonov , Michael Boyce-Jacino
发明人： Ming Xiao , Parikshit A. Deshpande , Han Cao , Michael Austin , Kandaswamy Vijayan , Alexey Y. Sharonov , Michael Boyce-Jacino
IPC分类号： C12Q1/68 , C07H1/00 , G01N33/53 , C07K1/107 , C12N9/96 , G01N30/96 , B82Y15/00
CPC分类号： C12Q1/6818 , C12Q1/6806 , C12Q1/6825 , C12Q1/6869 , G06F19/18 , C12Q2565/629 , C12Q2565/1015 , C12Q2521/301 , C12Q2537/1376 , C12Q2561/109 , C12Q2565/1025 , C12Q2565/631
摘要： Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g., determining structural variations and copy number variations between individuals.
摘要翻译：提供单分子基因组分析的方法和装置。在一个实施方案中，所述方法需要处理双链核酸并表征所述核酸。这些方法在例如确定个体之间的结构变化和拷贝数变化方面是有用的。

4. 发明授权

US07771944B2 Methods for determining genetic haplotypes and DNA mapping 有权
标题翻译：确定遗传单倍体和DNA作图的方法
公开(公告)号：US07771944B2
公开(公告)日：2010-08-10
申请号：US11957216
申请日：2007-12-14
申请人： Ming Xiao , Pui-Yan Kwok , Paul Selvin , Mathew Gordon
发明人： Ming Xiao , Pui-Yan Kwok , Paul Selvin , Mathew Gordon
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , C12Q1/6874 , C12Q2525/125 , C12Q2521/319 , C12Q2565/601 , C12Q2537/143
摘要： Improved methods of genetic haplotyping and DNA sequencing and mapping, including methods for making amplified ssDNA, methods for allele determination, and a DNA barcoding strategy based on direct imaging of individual DNA molecules and localization of multiple sequence motifs or polymorphic sites on a single DNA molecule.
摘要翻译：遗传单倍型和DNA测序和绘图的改进方法，包括制备扩增的ssDNA的方法，等位基因测定的方法和基于单个DNA分子的直接成像和多个序列基序或多态位点在单个DNA分子上的定位的DNA条形码策略。

5. 发明授权

US06323944B1 Compact spectrofluorometer 有权
标题翻译：紧凑型荧光分光光度计
公开(公告)号：US06323944B1
公开(公告)日：2001-11-27
申请号：US09443392
申请日：1999-11-19
申请人： Ming Xiao
发明人： Ming Xiao
IPC分类号： G01N2164
CPC分类号： G01J3/4406 , G01J3/02 , G01J3/0256 , G01J3/26 , G01J3/2803 , G01J2003/1213 , G01N21/645
摘要： Spectrofluorometer employing a pair of linear variable spectral filters to produce a three dimensional data output is disclosed. A collimated white light source is used that first passes through a first linear variable spectral filter, then through a sample where fluorescence occurs, then the resultant light passes through a second linear variable spectral light filter that is oriented at ninety degrees from the first filter. The light is then detected by a CCD sensor for conversion into data. This arrangement provides a very simple, rugged and compact instrument that can be used almost anywhere, such as at the scene of a contamination accident.
摘要翻译：公开了使用一对线性可变光谱滤波器产生三维数据输出的分光荧光计。使用准直的白光源，其首先通过第一线性可变光谱滤光器，然后通过荧光发生的样品，然后所得光通过从第一滤光器定向成90度的第二线性可变光谱滤光器。然后由CCD传感器检测光转换成数据。这种安排提供了一种非常简单，坚固耐用的紧凑型仪器，可以在几乎任何地方使用，例如在污染事故现场。

6. 发明申请

US20220352390A1 POWER SCHOTTKY BARRIER DIODES WITH HIGH BREAKDOWN VOLTAGE AND LOW LEAKAGE CURRENT 有权
公开(公告)号：US20220352390A1
公开(公告)日：2022-11-03
申请号：US17244394
申请日：2021-04-29
申请人： Yuhao ZHANG , Ming Xiao
发明人： Yuhao ZHANG , Ming Xiao
IPC分类号： H01L29/872 , H01L29/20 , H01L29/205 , H01L29/06 , H01L29/417 , H01L29/66
摘要： This disclosure provides a diode including a semiconductor region having at least one two-dimensional carrier channel of a first conductivity type. The diode also includes a plurality of sidewalls exposed in the semiconductor region defining at least one trench extending through the at least one two-dimensional carrier channel and a material of a second conductivity type, the second conductivity type being the other of the n-type and the p-type conductivity, disposed on the plurality of sidewalls and in contact with the at least one two-dimensional carrier channel. The diode also includes an anode material in contact with at least a portion of the semiconductor region and in contact with at least a portion of the material of the second conductivity type, and a cathode material in contact with the at least one two-dimensional carrier channel.

7. 发明申请

US20090155780A1 Methods for determining genetic haplotypes and DNA mapping 有权
标题翻译：确定遗传单倍体和DNA作图的方法
公开(公告)号：US20090155780A1
公开(公告)日：2009-06-18
申请号：US11957216
申请日：2007-12-14
申请人： Ming Xiao , Pui-Yan Kwok , Paul Selvin , Mathew Gordon
发明人： Ming Xiao , Pui-Yan Kwok , Paul Selvin , Mathew Gordon
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , C12Q1/6874 , C12Q2525/125 , C12Q2521/319 , C12Q2565/601 , C12Q2537/143
摘要： Improved methods of genetic haplotyping and DNA sequencing and mapping, including methods for making amplified ssDNA, methods for allele determination, and a DNA barcoding strategy based on direct imaging of individual DNA molecules and localization of multiple sequence motifs or polymorphic sites on a single DNA molecule.
摘要翻译：遗传单倍型和DNA测序和绘图的改进方法，包括制备扩增的ssDNA的方法，等位基因测定的方法和基于单个DNA分子的直接成像和多个序列基序或多态位点在单个DNA分子上的定位的DNA条形码策略。

8. 发明申请

US20060163240A1 Mini scented wax heating device 审中-公开
标题翻译：迷你香味蜡加热装置
公开(公告)号：US20060163240A1
公开(公告)日：2006-07-27
申请号：US11042727
申请日：2005-01-24
申请人： Ming Xiao
发明人： Ming Xiao
IPC分类号： F27D11/00
CPC分类号： A61L9/03
摘要： A mini scented wax heating device includes a heating cylinder which is in a penetrating cylindrical form and installs a heating device therein. The heating device comprises a heat plate and a ceramic heating piece attached to the heat plate, and the heat plate is latched to a fixed end of the heating cylinder. The invention uses the heat plate to directly heat the bottom of the heating device. Since the heating device is a mini heating device that heats the heat plate directly by the ceramic heating piece, the heat plate can directly heat and vaporize a piece of scented wax. The invention increases the heating area of the scented wax by the heater, shortens the heating time, and evenly heats the scented wax in the heater, and thus can achieve a better vaporization effect. The invention features a simple structure, a low cost, and a powerful function.
摘要翻译：迷你香味蜡加热装置包括加热缸，该加热缸呈贯穿圆柱形并在其中安装加热装置。加热装置包括加热板和附接到加热板的陶瓷加热片，并且加热板被锁定到加热缸的固定端。本发明使用加热板直接加热加热装置的底部。由于加热装置是通过陶瓷加热片直接加热加热板的微型加热装置，所以加热板可以直接加热和蒸发一块香味的蜡。本发明通过加热器增加了香味蜡的加热面积，缩短了加热时间，并且均匀地加热加热器中的香味蜡，从而可以获得更好的蒸发效果。本发明具有结构简单，成本低，功能强大的特点。

9. 发明授权

US5409206A Apparatus for buffering transport of documents 失效
标题翻译：用于缓冲文件传输的装置
公开(公告)号：US5409206A
公开(公告)日：1995-04-25
申请号：US174870
申请日：1993-12-29
申请人： Joseph H. Marzullo , Steven A. Supron , Brian S. Thompson , Ming Xiao
发明人： Joseph H. Marzullo , Steven A. Supron , Brian S. Thompson , Ming Xiao
IPC分类号： B65H29/42
CPC分类号： B65H29/42 , B65H2601/251
摘要： An apparatus for buffering transport of freshly inked documents is comprised of an upper deck oriented at an acute angle with respect to a horizontal plane. The apparatus has a plurality of opposite, parallel, threaded screw conveyers orthogonal to the upper deck. Inked documents travel upon the threads of the screw conveyers. A lower deck is provided for receiving inked documents. An eject pin mechanism causes the inked documents to be delivered from the apparatus.
摘要翻译：用于缓冲新鲜印刷的文件的运送的装置包括相对于水平面以锐角取向的上甲板。该装置具有与上甲板正交的多个相对的平行螺纹输送机。润滑的文件在螺旋输送机的螺纹上行进。提供下层甲板用于接收墨水文件。排出销机构使着墨文件从设备传送。

10. 发明授权

US5203555A Adjustable dampening device in an apparatus for detecting double fed sheets 失效
标题翻译：用于检测双馈纸的装置中的可调节阻尼装置
公开(公告)号：US5203555A
公开(公告)日：1993-04-20
申请号：US941274
申请日：1992-09-04
申请人： Joseph A. Cannaverde , Joseph H. Marzullo , Ming Xiao
发明人： Joseph A. Cannaverde , Joseph H. Marzullo , Ming Xiao
IPC分类号： B65H7/12
CPC分类号： B65H7/12 , B65H2511/13 , B65H2511/212 , B65H2511/524 , B65H2553/61
摘要： In a mechanism for detecting double sheets fed from a feeder, including an actuator arm operatively coupled at one end to a magnet and at the other end to a paper follower, wherein the paper follower follows the profile of each sheet or group of sheets being conveyed between the paper follower and a fixed transport deck below thereto, the paper follower causing the actuator arm to pivot about a first axis to raise and lower the magnet with respect to a stationary electromagnetic sensor, an improvement comprises a flat spring mounted at one end to a shaft extending along the first axis, and a lever, having a pin for supporting an other end of the flat spring. The lever is pivotally mounted along a second axis for pivoting the pin between at least a first and second position wherein the flat spring provides a first dampening load to the paper follower and the actuator arm when the lever is in the fist position and provides a second dampening load to the actuator arm when the lever is in the second position.
摘要翻译：在用于检测从进给器进给的双页纸的机构中，包括一端可操作地连接到磁体的致动器臂，另一端连接到纸张从动件，其中纸张随动件遵循被传送的每张纸张或一组纸张的轮廓在纸从动件与其下方的固定运输甲板之间，纸张随动件使致动器臂相对于固定电磁传感器绕第一轴线枢转以升高和降低磁体，改进包括安装在一端至沿着第一轴线延伸的轴，以及具有用于支撑板簧的另一端的销的杆。所述杠杆沿着第二轴线可枢转地安装，用于在至少第一和第二位置之间枢转所述销，其中当所述杠杆处于所述第一位置时，所述弹簧向所述随动件和所述致动器臂提供第一阻尼载荷，并提供第二当杠杆处于第二位置时，减轻执行器臂的负载。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式