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1. 发明申请

US20100143955A1 REAGENT FOR BLOOD ANALYSIS AND METHOD OF USING THE SAME 有权
标题翻译：用于血液分析的试剂及其使用方法
公开(公告)号：US20100143955A1
公开(公告)日：2010-06-10
申请号：US12633452
申请日：2009-12-08
申请人： Zhang Baohua , Xu Bing , Kuang Yuji , Lei Ting , Shao Jianhui , Bao Dequan
发明人： Zhang Baohua , Xu Bing , Kuang Yuji , Lei Ting , Shao Jianhui , Bao Dequan
IPC分类号： C12Q1/54 , C12Q1/02 , C12Q1/06
CPC分类号： G01N33/52 , G01N15/1459 , G01N33/5094 , G01N2015/1006 , G01N2015/1402
摘要： A reagent for blood analysis includes: (1) a compound having the general formula I; and (2) at least one surfactant selected from cationic surfactants and nonionic surfactants. In another aspect a method for differentiating and counting blood cells is provided, the method includes the following steps: (a) mixing a blood sample with the reagent for blood analysis according to the present disclosure to form a cell suspension; (b) detecting scattered light signals and fluorescence signals of cells in the blood sample; and (c) differentiating and counting the cells in the blood sample based upon the scattered light signals and fluorescence signals. The reagent for blood analysis may be effective for identifying and counting erythroblasts and/or basophils in a blood sample to be detected, and meanwhile counting leukocytes therein.
摘要翻译：用于血液分析的试剂包括：（1）具有通式I的化合物; 和（2）至少一种选自阳离子表面活性剂和非离子表面活性剂的表面活性剂。另一方面，提供了用于分化和计数血细胞的方法，该方法包括以下步骤：（a）根据本公开将血液样品与用于血液分析的试剂混合以形成细胞悬浮液; （b）检测血液样品中细胞的散射光信号和荧光信号; 和（c）基于散射光信号和荧光信号对血液样品中的细胞进行微分和计数。用于血液分析的试剂可以有效地鉴定和计数要检测的血液样品中的成红细胞和/或嗜碱性粒细胞，同时计数其中的白细胞。

2. 发明授权

US08367358B2 Reagent, kit and method for differentiating and counting leukocytes 有权
标题翻译：用于分化和计数白细胞的试剂，试剂盒和方法
公开(公告)号：US08367358B2
公开(公告)日：2013-02-05
申请号：US12568500
申请日：2009-09-28
申请人： Lei Ting , Xu Bing , Kuang Yuji , Zhang Baohua , Shao Jianhui
发明人： Lei Ting , Xu Bing , Kuang Yuji , Zhang Baohua , Shao Jianhui
IPC分类号： C07D403/08 , C12Q1/68 , G01N1/30
CPC分类号： C07D417/06 , G01N33/5094 , G01N33/532 , G01N33/56972 , G01N33/582
摘要： The present disclosure discloses a reagent for differentiating and counting leukocytes which includes: (1) cationic cyanine compounds selected from those having the following general formulae I and II; (2) cationic surfactants selected from those having the following general formulae III, IV and/or V; (3) at least one nonionic surfactant; and (4) optionally, at least one anionic compound selected from those having one or more carboxyl or sulphonyl groups; and optionally includes alcohol compounds. Also disclosed is a kit comprising the reagent for differentiating and counting leukocytes. Further disclosed is a method for differentiating and counting leukocytes using the reagent and kit. Use of the reagent, kit and method disclosed enables the identification of leukocytes in blood samples into five subtypes in a very short time, or at least achieves the differentiation and counting of four leukocyte groupings. Moreover, immature and abnormal cells can be identified.
摘要翻译：本公开公开了一种用于白细胞分化和计数的试剂，其包括：（1）选自具有以下通式I和II的那些的阳离子花青化合物; （2）选自具有以下通式III，IV和/或V的阳离子表面活性剂的阳离子表面活性剂; （3）至少一种非离子表面活性剂; 和（4）任选的至少一种选自具有一个或多个羧基或磺酰基的阴离子化合物; 并且任选地包括醇化合物。还公开了包含用于分化和计数白细胞的试剂的试剂盒。进一步公开了使用试剂和试剂盒对白细胞进行微分和计数的方法。使用所公开的试剂，试剂盒和方法使得能够在非常短的时间内将血液样品中的白细胞鉴定为五种亚型，或至少实现四种白细胞分组的分化和计数。此外，可以鉴定未成熟和异常细胞。

3. 发明授权

US08334109B2 Reagent for blood analysis and method of using the same 有权
标题翻译：血液分析用试剂及其使用方法
公开(公告)号：US08334109B2
公开(公告)日：2012-12-18
申请号：US12633452
申请日：2009-12-08
申请人： Zhang Baohua , Xu Bing , Kuang Yuji , Lei Ting , Shao Jianhui , Bao Dequan
发明人： Zhang Baohua , Xu Bing , Kuang Yuji , Lei Ting , Shao Jianhui , Bao Dequan
IPC分类号： C07D403/08 , C12Q1/68 , G01N1/30
CPC分类号： G01N33/52 , G01N15/1459 , G01N33/5094 , G01N2015/1006 , G01N2015/1402
摘要： A reagent for blood analysis includes: (1) a compound having the general formula I; and (2) at least one surfactant selected from cationic surfactants and nonionic surfactants. In another aspect a method for differentiating and counting blood cells is provided, the method includes the following steps: (a) mixing a blood sample with the reagent for blood analysis according to the present disclosure to form a cell suspension; (b) detecting scattered light signals and fluorescence signals of cells in the blood sample; and (c) differentiating and counting the cells in the blood sample based upon the scattered light signals and fluorescence signals. The reagent for blood analysis may be effective for identifying and counting erythroblasts and/or basophils in a blood sample to be detected, and meanwhile counting leukocytes therein.
摘要翻译：用于血液分析的试剂包括：（1）具有通式I的化合物; 和（2）至少一种选自阳离子表面活性剂和非离子表面活性剂的表面活性剂。另一方面，提供了用于分化和计数血细胞的方法，该方法包括以下步骤：（a）根据本公开将血液样品与用于血液分析的试剂混合以形成细胞悬浮液; （b）检测血液样品中细胞的散射光信号和荧光信号; 和（c）基于散射光信号和荧光信号对血液样品中的细胞进行微分和计数。用于血液分析的试剂可以有效地鉴定和计数要检测的血液样品中的成红细胞和/或嗜碱性粒细胞，同时计数其中的白细胞。

4. 发明申请

US20100151509A1 REAGENT, KIT AND METHOD FOR DIFFERENTATING AND COUNTING LEUKOCYTES 有权
标题翻译：试剂，试剂盒和方法用于分离和计数白藜芦醇
公开(公告)号：US20100151509A1
公开(公告)日：2010-06-17
申请号：US12568500
申请日：2009-09-28
申请人： Lei Ting , Xu Bing , Kuang Yuji , Zhang Baohua , Shao Jianhui
发明人： Lei Ting , Xu Bing , Kuang Yuji , Zhang Baohua , Shao Jianhui
IPC分类号： C12Q1/02 , C12N5/02
CPC分类号： C07D417/06 , G01N33/5094 , G01N33/532 , G01N33/56972 , G01N33/582
摘要： The present disclosure discloses a reagent for differentiating and counting leukocytes which includes: (1) cationic cyanine compounds selected from those having the following general formulae I and II; (2) cationic surfactants selected from those having the following general formulae III, IV and/or V; (3) at least one nonionic surfactant; and (4) optionally, at least one anionic compound selected from those having one or more carboxyl or sulphonyl groups; and optionally includes alcohol compounds. Also disclosed is a kit comprising the reagent for differentiating and counting leukocytes. Further disclosed is a method for differentiating and counting leukocytes using the reagent and kit. Use of the reagent, kit and method disclosed enables the identification of leukocytes in blood samples into five subtypes in a very short time, or at least achieves the differentiation and counting of four leukocyte groupings. Moreover, immature and abnormal cells can be identified.
摘要翻译：本公开公开了一种用于白细胞分化和计数的试剂，其包括：（1）选自具有以下通式I和II的那些的阳离子花青化合物; （2）选自具有以下通式III，IV和/或V的阳离子表面活性剂的阳离子表面活性剂; （3）至少一种非离子表面活性剂; 和（4）任选的至少一种选自具有一个或多个羧基或磺酰基的阴离子化合物; 并且任选地包括醇化合物。还公开了包含用于分化和计数白细胞的试剂的试剂盒。进一步公开了使用试剂和试剂盒对白细胞进行微分和计数的方法。使用所公开的试剂，试剂盒和方法使得能够在非常短的时间内将血液样品中的白细胞鉴定为五种亚型，或至少实现四种白细胞分组的分化和计数。此外，可以鉴定未成熟和异常细胞。

5. 发明申请

US20090305285A1 ASYMMETRIC CYANINE COMPOUNDS, THEIR PREPARATION METHODS AND THEIR USES 有权
标题翻译：不对称氰基化合物，其制备方法及其用途
公开(公告)号：US20090305285A1
公开(公告)日：2009-12-10
申请号：US12482335
申请日：2009-06-10
申请人： Shao Jianhui
发明人： Shao Jianhui
IPC分类号： C12Q1/68 , C07D417/06 , C12Q1/02
CPC分类号： C07D417/06 , Y10S436/80 , Y10T436/14
摘要： Asymmetric cyanine compounds represented by general formula I are provided, wherein X, n, R1, R2, R3, R4 and Y− are as defined in the specification. They have a maximum absorption peak at about 640 nm which may not change with ambient temperature. When the compounds bind a nucleic acid to form a dye/nucleic acid complex, the fluorescence intensity of the complexes will increased rapidly, so that they can be used as a staining agent for nucleic acids in flow cytometers. Their spectra are in the near-infrared region, which can effectively reduce the interference from background fluorescence and improve the accuracy of detection. Moreover, the compounds provided can also be used as a staining agent for blood reticulocytes.
摘要翻译：提供由通式I表示的不对称花青化合物，其中X，n，R 1，R 2，R 3，R 4和Y-如说明书中所定义。它们在约640nm处具有最大吸收峰，这可能不随环境温度而变化。当化合物结合核酸以形成染料/核酸复合物时，络合物的荧光强度将快速增加，因此它们可以用作流式细胞仪中核酸的染色剂。它们的光谱位于近红外区域，可有效降低背景荧光的干扰，提高检测精度。此外，所提供的化合物也可以用作血液网织红细胞的染色剂。

6. 发明授权

US07709653B2 Asymmetric cyanine compounds, their preparation methods and their uses 有权
标题翻译：不对称花青化合物，其制备方法及用途
公开(公告)号：US07709653B2
公开(公告)日：2010-05-04
申请号：US12482335
申请日：2009-06-10
申请人： Shao Jianhui
发明人： Shao Jianhui
IPC分类号： C07D293/10 , G01N33/00 , C12Q1/68
CPC分类号： C07D417/06 , Y10S436/80 , Y10T436/14
摘要： Asymmetric cyanine compounds represented by general formula I are provided, wherein X, n, R1, R2, R3, R4 and Y− are as defined in the specification. They have a maximum absorption peak at about 640 nm which may not change with ambient temperature. When the compounds bind a nucleic acid to form a dye/nucleic acid complex, the fluorescence intensity of the complexes will increased rapidly, so that they can be used as a staining agent for nucleic acids in flow cytometers. Their spectra are in the near-infrared region, which can effectively reduce the interference from background fluorescence and improve the accuracy of detection. Moreover, the compounds provided can also be used as a staining agent for blood reticulocytes.
摘要翻译：提供由通式I表示的不对称花青化合物，其中X，n，R 1，R 2，R 3，R 4和Y-如说明书中所定义。它们在约640nm处具有最大吸收峰，这可能不随环境温度而变化。当化合物结合核酸以形成染料/核酸复合物时，络合物的荧光强度将快速增加，因此它们可以用作流式细胞仪中核酸的染色剂。它们的光谱位于近红外区域，可有效降低背景荧光的干扰，提高检测精度。此外，所提供的化合物也可以用作血液网织红细胞的染色剂。

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