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1. 发明公开

EP1286164A2 Methods and apparatus for improved luminescence assays 失效
标题翻译： Verfahren und Vorrichtungfürverbesserte Lumineszenztests
公开(公告)号：EP1286164A2
公开(公告)日：2003-02-26
申请号：EP02022214.7
申请日：1992-02-05
申请人： IGEN International, Inc.
发明人： Leland, John K. , Kenten, John H. , Lowke, George E. , Shah, Haresh P. , Goodman, Jack E. , Blackburn, Gary F. , Massey, Richard J.
IPC分类号： G01N33/543 , G01N21/76
CPC分类号： G01N21/69 , C07H21/00 , C12Q1/6825 , G01N1/40 , G01N21/66 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N2458/30
摘要： The invention relates to an apparatus for performing a binding assay for an analyte of interest in a sample based upon measurement of electro-chemiluminescence at an electrode surface comprising an electrochemical cell having a sample-holding volume, inlet and outlet means; a working electrode system to provide electrochemical energy to trigger electrochemiluminescence; a voltage control device to supply voltage signals to the working electrode system; a magnet placed in proper orientation and in close proximity to the working electrode so that magnetically responsive particles will concentrate on the surface of the working electrode; a light detection/measurement device for measuring electrochemiluminescence triggered at the electrode surface; and a pump to provide for fluid transport to, through and from the electrochemical cell. The apparatus may be used in an electrochemiluminescence assay for an analyte of interest in which magnetically responsive particles are magnetically collected on the electrode surface and emitted electrochemiluminescence is measured using the light detector.
摘要翻译：本发明涉及一种用于对包含具有样品保持体积的电化学电池，入口和出口装置的电极表面上的电化学发光测量来对样品中感兴趣的分析物进行结合测定的装置; 提供电化学能量以触发电化学发光的工作电极系统; 电压控制装置，用于向工作电极系统提供电压信号; 放置在正确定向并且靠近工作电极的磁体，使得磁响应颗粒将集中在工作电极的表面上; 用于测量在电极表面触发的电化学发光的光检测/测量装置; 以及用于提供流体输送到，通过和来自电化学电池的泵。该装置可以用于感兴趣的分析物的电化学发光测定中，其中磁响应颗粒被磁性收集在电极表面上，并且使用光检测器测量发射的电化学发光。

2. 发明公开

EP1286164A3 Methods and apparatus for improved luminescence assays 失效
标题翻译：用于改进发光测定的方法和设备
公开(公告)号：EP1286164A3
公开(公告)日：2003-10-22
申请号：EP02022214.7
申请日：1992-02-05
申请人： IGEN International, Inc.
发明人： Leland, John K. , Kenten, John H. , Lowke, George E. , Shah, Haresh P. , Goodman, Jack E. , Blackburn,Gary F. , Massey, Richard J.
IPC分类号： G01N33/543 , G01N21/76 , G01N21/66
CPC分类号： G01N21/69 , C07H21/00 , C12Q1/6825 , G01N1/40 , G01N21/66 , G01N21/76 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/582 , G01N2458/30
摘要： The invention relates to an apparatus for performing a binding assay for an analyte of interest in a sample based upon measurement of electro-chemiluminescence at an electrode surface comprising an electrochemical cell having a sample-holding volume, inlet and outlet means; a working electrode system to provide electrochemical energy to trigger electrochemiluminescence; a voltage control device to supply voltage signals to the working electrode system; a magnet placed in proper orientation and in close proximity to the working electrode so that magnetically responsive particles will concentrate on the surface of the working electrode; a light detection/measurement device for measuring electrochemiluminescence triggered at the electrode surface; and a pump to provide for fluid transport to, through and from the electrochemical cell. The apparatus may be used in an electrochemiluminescence assay for an analyte of interest in which magnetically responsive particles are magnetically collected on the electrode surface and emitted electrochemiluminescence is measured using the light detector.
摘要翻译：本发明涉及一种用于基于在电极表面处测量电化学发光来执行对样品中感兴趣分析物的结合测定的装置，所述电化学发光包括具有样品保持体积的电化学电池，入口和出口装置; 提供电化学能量以触发电化学发光的工作电极系统; 向工作电极系统提供电压信号的电压控制装置; 放置在适当取向并且靠近工作电极的磁体，使得磁响应粒子将集中在工作电极的表面上; 用于测量在电极表面触发的电化学发光的光检测/测量装置; 以及提供流体输送到电化学电池，通过电化学电池和从电化学电池输送的泵。该设备可以用于电化学发光测定中，用于感兴趣的分析物，其中磁响应粒子被磁性地收集在电极表面上并且使用光检测器测量发射的电化学发光。

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