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1. 发明授权

US5567302A Electrochemical system for rapid detection of biochemical agents that catalyze a redox potential change 失效
标题翻译：用于快速检测催化氧化还原电位变化的生物化学试剂的电化学系统
公开(公告)号：US5567302A
公开(公告)日：1996-10-22
申请号：US483249
申请日：1995-06-07
申请人： Herking Song , Dean G. Hafeman
发明人： Herking Song , Dean G. Hafeman
IPC分类号： G01N27/30 , G01N27/403 , G01N27/26
CPC分类号： G01N27/3277 , Y10S435/817
摘要： The present invention relates to a system for detecting, in a reliable, precise and highly sensitive manner, biochemical agents such as enzymes that catalyze a redox potential change. One electrode is used to measure redox potential changes in an aqueous electrolyte containing the biochemical agents. Another electrode is used to deliver a feedback current to the electrolyte in response to measured Changes in electrolyte redox potential. The amount of feedback current or charge delivered through the electrode to the electrolyte is sufficient in magnitude to maintain a constant redox potential. Quantitation of the amount of feedback current or charge necessary to maintain the constant redox potential may then be used to determine the amount of biochemical agents present. Alternatively, the redox potential need not be kept constant, but instead may be allowed to reach a new steady-state. Thus, the current, or charge, conducted by a feedback electrode to maintain a new steady-state potential in the presence of an enzymatic reaction may be used to quantitate the amount of enzymatic activity present. The present invention provides precision in the quantitation results, high sensitivity in the enzyme detection, and a wider dynamic range for quantitation of the biochemical agent.
摘要翻译：本发明涉及一种用于以可靠，精确和高度敏感的方式检测催化氧化还原电位变化的生物化学试剂例如酶的系统。一个电极用于测量含有生物化学试剂的含水电解质中的氧化还原电位变化。响应于测量的电解质氧化还原电位的变化，另一电极用于向电解质输送反馈电流。通过电极传递到电解质的反馈电流或电荷的量足以维持恒定的氧化还原电位。然后可以使用定量维持恒定氧化还原电位所需的反馈电流或电荷量来确定存在的生物化学试剂的量。或者，氧化还原电位不需要保持恒定，而是可以允许达到新的稳态。因此，可以使用由反馈电极进行的在酶反应存在下维持新的稳态电位的电流或电荷来定量存在的酶活性的量。本发明提供了定量结果的精确性，酶检测的高灵敏度和用于定量生物化学试剂的更广的动态范围。

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