专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2011105071A1 METHOD FOR SCREENING NUCLEIC ACID LIGAND 审中-公开
标题翻译：筛选核酸配体的方法
公开(公告)号：WO2011105071A1
公开(公告)日：2011-09-01
申请号：PCT/JP2011/001024
申请日：2011-02-23
申请人： CANON KABUSHIKI KAISHA , HATAKEYAMA, Satoru , IBII, Takahisa
发明人： HATAKEYAMA, Satoru , IBII, Takahisa
IPC分类号： C12Q1/68 , C12N15/09 , G01N33/53
CPC分类号： C12N15/1048 , G01N33/5308
摘要： Provided is a method for screening a library of candidate for a nucleic acid ligand. The method includes the steps of: (a) preparing a library of candidate of nucleic acid ligands; (b) contacting, under the absence of a target substance, the library with a supporting member binding to at least one of conserved sequence domains included in the ligand, and then separating and removing a ligand which does not form an intermolecular duplex; and (c) dissociating the intermolecular duplex by contacting, under the presence of the target substance, the target substance with the remaining ligand forming the intermolecular duplex obtained in step (b), and then separating and collecting a ligand having a specific secondary structure formed by the binding to the target substance, wherein the method includes at least one time of step (b) and at least one time of step (c).
摘要翻译：提供了筛选核酸配体候选文库的方法。该方法包括以下步骤：（a）制备核酸配体候选文库; （b）在不存在目标物质的情况下，使文库与支配构件接触，所述支持构件与包含在配体中的保守序列结构域中的至少一个结合，然后分离和除去不形成分子间双键的配体; 和（c）通过在靶物质的存在下使靶物质与形成步骤（b）中获得的分子间双环体的剩余配体接触，然后分离和收集形成具有特定二级结构的配体，从而解离分子间双链体通过与目标物质的结合，其中所述方法包括步骤（b）的至少一个时间和步骤（c）的至少一个时间）。

2. 发明申请

WO2007026972A3 BINDING PROTEIN MOLECULE 审中-公开
公开(公告)号：WO2007026972A3
公开(公告)日：2007-03-08
申请号：PCT/JP2006/317808
申请日：2006-09-01
申请人： CANON KABUSHIKI KAISHA , IMAMURA, Takeshi , SHIOTSUKA, Hidenori , BAN, Kazuhiro , IBII, Takahisa
发明人： IMAMURA, Takeshi , SHIOTSUKA, Hidenori , BAN, Kazuhiro , IBII, Takahisa
IPC分类号： C07K16/40 , C07K16/18 , C07K16/44 , C07K17/14 , G01N33/553
摘要： A binding protein molecule, characterized in that it has a first domain having a binding site to an inhibitor of non-specific adsorption in which the domain comprises a part of the variable region of an antibody as the binding site and a second domain having a binding site to a target substance in which the domain comprises a part of the variable region of an antibody as the binding site, wherein the first and second domains are bound via a linker.

3. 发明公开

EP2494080A1 METHOD FOR SCREENING NUCLEIC ACID LIGAND 审中-公开
标题翻译：一种筛选核酸配体
公开(公告)号：EP2494080A1
公开(公告)日：2012-09-05
申请号：EP11747040.1
申请日：2011-02-23
申请人： Canon Kabushiki Kaisha
发明人： HATAKEYAMA, Satoru , IBII, Takahisa
IPC分类号： C12Q1/68 , C12N15/09 , G01N33/53
CPC分类号： C12N15/1048 , G01N33/5308
摘要： Provided is a method for screening a library of candidate for a nucleic acid ligand. The method includes the steps of: (a) preparing a library of candidate of nucleic acid ligands; (b) contacting, under the absence of a target substance, the library with a supporting member binding to at least one of conserved sequence domains included in the ligand, and then separating and removing a ligand which does not form an intermolecular duplex; and (c) dissociating the intermolecular duplex by contacting, under the presence of the target substance, the target substance with the remaining ligand forming the intermolecular duplex obtained in step (b), and then separating and collecting a ligand having a specific secondary structure formed by the binding to the target substance, wherein the method includes at least one time of step (b) and at least one time of step (c).

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式