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    • 6. 发明专利
    • Method for controlling the microbiological quality of an aqueous medium and kit therefor
    • NZ552462A
    • 2008-09-26
    • NZ55246201
    • 2001-07-06
    • BIO MERIEUX
    • RENAUD PATRICIAGUILLOT EMMANUELLEMABILAT CLAUDEVACHON CAROLELACROIX BRUNOVERNET GUYARMAND MARIE-ASTRIDLAFFAIRE PHILIPE
    • C12N15/09C12Q1/68G01N33/53C12Q1/689C12Q1/6893C12Q1/70C12R1/19G01N33/18G01N33/569G01N37/00
    • Disclosed is a method for establishing the microbiological quality of an aqueous environmental medium, liable to comprise various microorganisms, comprising the steps of: providing a sample to be tested and a reference set, consisting of at least a bacterium, a parasite and a virus, representative, together or separately of a level of microbiological quality; utilising a kit for microbiological determination is available, consisting of at least one identifying probe specific for a bacterium, said identifying probe being chosen from SEQ ID NO:1 to SEQ ID NO:39, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:66 to SEQ ID NO: 69 and any fragments thereof comprising at least 5 contiguous monomers included in any one of said sequences, and having a sequence exhibiting at least 70% identity with said any sequence; at least one identifying probe specific for a parasite said identifying probe being chosen from SEQ ID NO: 40 to SEQ ID NO:49, SEQID NO:63 to SEQ ID NO:65 and any fragments thereof comprising at least 5 contiguous monomers included in any one of said sequences, and having a sequence exhibiting at least 70% identity with said any sequence; and at least one identifying probe specific for a virus, said identifying probe being chosen from SEQ ID NO:50 to SEQ ID NO:60, SEQ ID NO:70, SEQ ID NO:104 and any fragments thereof comprising at least 5 contiguous monomers included in any one of said sequences, and having a sequence exhibiting at least 70% identity with said any sequence; wherein the sample and the reference set, or any fraction obtained from the latter, are brought into contact with said determination kit as a result of which, any microorganisms are detected, wherein this determination is representative of the level of microbiological quality of the medium.