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1. 发明申请

WO2012017008A1 A NOVEL GLUCOSE OXIDASE 审中-公开
标题翻译：新型葡萄糖氧化酶
公开(公告)号：WO2012017008A1
公开(公告)日：2012-02-09
申请号：PCT/EP2011/063368
申请日：2011-08-03
申请人： B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG , SCHWANEBERG, Ulrich , PRODANOVIC, Radivoje , OSTAFE, Raluca , NIEHAUS, Frank , ECK, Jürgen
发明人： SCHWANEBERG, Ulrich , PRODANOVIC, Radivoje , OSTAFE, Raluca , NIEHAUS, Frank , ECK, Jürgen
IPC分类号： C12N9/04
CPC分类号： C12N9/0006
摘要： The present invention relates to a novel nucleic acid molecule encoding (I) a polypeptide having the activity of a glucose oxidase (EC 1.1.3.4); or (II) a fragment of the polypeptide of (I) having the activity of a glucose oxidase and comprising at least 400 amino acids. Moreover, the invention relates to a vector encoding the nucleic acid molecule, a host cell transformed, transduced or transfected with the vector, a the protein encoded by the nucleic acid molecule, and compositions comprising said nucleic acid molecule, vector, host cell, protein or combinations thereof.
摘要翻译：本发明涉及编码（I）具有葡萄糖氧化酶活性的多肽的新型核酸分子（EC 1.1.3.4）; 或（II）具有葡萄糖氧化酶活性且包含至少400个氨基酸的（I）多肽的片段。此外，本发明涉及编码核酸分子的载体，用载体转化，转导或转染的宿主细胞，由核酸分子编码的蛋白质，以及包含所述核酸分子，载体，宿主细胞，蛋白质的组合物或其组合。

2. 发明申请

WO2012028709A2 NOVEL MONOOXYGENASE VARIANTS 审中-公开
标题翻译：新奥尔良的变化
公开(公告)号：WO2012028709A2
公开(公告)日：2012-03-08
申请号：PCT/EP2011/065179
申请日：2011-09-02
申请人： B.R.A.I.N. Biotechnology Research And Information Network AG , SCHWANEBERG, Ulrich , BLANUSA, Milan , NIEHAUS, Frank , ECK, Jürgen
发明人： SCHWANEBERG, Ulrich , BLANUSA, Milan , NIEHAUS, Frank , ECK, Jürgen
IPC分类号： C12N9/02
CPC分类号： C12Y106/02004 , C12N9/0042
摘要： The present invention relates to a nucleic acid molecule encoding a polypeptide having cytochrome P450 monooxygenase activity, wherein said polypeptide comprises a reductase domain that deviates by at least one mutation from (a) the reductase domain of cytochrome P450 BM3, wherein the reductase domain of cytochrome P450 BM3 is represented by SEQ ID NO: 1; or (b) a reductase domain having at least 95% sequence identity to SEQ ID NO:1; and wherein said mutation(s) result(s) in an increased cytochrome P450 monooxygenase activity as compared to a polypeptide comprising the reductase domain of SEQ ID NO: 1. The present invention also relates to a vector comprising the nucleic acid molecule of the invention and a host transformed with the vector. Furthermore, the invention relates to a method of producing a polypeptide comprising culturing the host of the invention as well as to a polypeptide encoded by the nucleic acid molecule of the invention or produced by the method of the invention. The present invention further relates to the use of the polypeptide of the invention in biotransformation or fine chemical synthesis, to an oligo- or polynucleotide which specifically hybridizes to the nucleic acid molecule of the invention as well as to a composition and to a kit.
摘要翻译：本发明涉及编码具有细胞色素P450单加氧酶活性的多肽的核酸分子，其中所述多肽包含通过至少一个突变从（a）细胞色素P450 BM3的还原酶结构域偏移的还原酶结构域，其中细胞色素的还原酶结构域 P450 BM3由SEQ ID NO：1表示; 或（b）与SEQ ID NO：1具有至少95％序列同一性的还原酶结构域; 并且其中与包含SEQ ID NO：1的还原酶结构域的多肽相比，增加的细胞色素P450单加氧酶活性的所述突变结果。本发明还涉及包含本发明的核酸分子的载体和一个用矢量转换的主机。此外，本发明涉及产生多肽的方法，其包括培养本发明的宿主以及由本发明的核酸分子编码或通过本发明的方法生产的多肽。本发明还涉及本发明的多肽在生物转化或精细化学合成中与本发明的核酸分子以及组合物和试剂盒特异性杂交的寡核苷酸或多核苷酸的用途。

3. 发明申请

WO2004106527A1 ARCHAEON EXPRESSION SYSTEM 审中-公开
标题翻译： ARCHAEON表达系统
公开(公告)号：WO2004106527A1
公开(公告)日：2004-12-09
申请号：PCT/EP2004/005936
申请日：2004-06-02
申请人： B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG , SCHLEPER, Christa , JONUSCHEIT, Melanie , ECK, Jürgen , NIEHAUS, Frank , ALBERS, Sonja-Verena , FROELS, Sabrina
发明人： SCHLEPER, Christa , JONUSCHEIT, Melanie , ECK, Jürgen , NIEHAUS, Frank , ALBERS, Sonja-Verena , FROELS, Sabrina
IPC分类号： C12N15/74
CPC分类号： C12N15/74
摘要： The present invention relates to a sulfolobus expression vector comprising: (a) sulfolobus origin of replication; (b) the genes encoding the structural proteins and the site-specific integrase of SSVI, SSV2 or pSSVx, operatively linked to expression control sequences and a packaging signal; (c) one or more selectable marker gene(s), operatively linked to sulfolobus expression control sequences; and (d) a sulfolobus promoter followed 3' by a restriction enzyme recognition site or a multiple cloning site for insertion of a gene of interest and optionally a 3' regulatory element. Moreover, the present invention relates to a shuttle vector comprising the sequences of the expression vector of the invention and additional sequences for propagation and selection in E. coli, wherein the additional sequences comprise (a) an E.coli on of replication; and (b) a marker for selection in E.coli. Furthermore, the invention relates to host cells transformed with the expression vector as well as to a kit comprising a vector or a host cell of the present invention. Finally, the present application also relates to a method for generating infectious subviral particles.
摘要翻译：本发明涉及一种磺基蛇毒表达载体，其包含：（a）磺基链复制起点; （b）编码与表达控制序列和包装信号有效连接的SSVI，SSV2或pSSVx的结构蛋白和位点特异性整合酶的基因; （c）一个或多个选择性标记基因，其可操作地连接到子叶表达控制序列; 和（d）通过限制性酶识别位点或用于插入目的基因和任选的3'调节元件的多克隆位点的3'后的磺基叶酸启动子。此外，本发明涉及包含本发明的表达载体的序列和在大肠杆菌中用于繁殖和选择的其它序列的穿梭载体，其中所述另外的序列包含（a）复制的大肠杆菌; 和（b）在大肠杆菌中选择的标记物。此外，本发明涉及用表达载体转化的宿主细胞以及包含本发明的载体或宿主细胞的试剂盒。最后，本申请还涉及产生感染性亚病毒颗粒的方法。

4. 发明申请

WO2004018673A2 ISOLATION AND CLONING OF DNA FROM UNCULTIVATED ORGANISMS 审中-公开
标题翻译：来自未受污染的生物体的DNA的分离和克隆
公开(公告)号：WO2004018673A2
公开(公告)日：2004-03-04
申请号：PCT/EP2003/009223
申请日：2003-08-20
申请人： B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG , QUAISER, Achim , OCHSENREITER, Torsten , TREUSCH, Alexander, H. , KLETZIN, Arnulf , SCHLEPER, Christa , LORENZ, Patrick , ECK, Jürgen
发明人： QUAISER, Achim , OCHSENREITER, Torsten , TREUSCH, Alexander, H. , KLETZIN, Arnulf , SCHLEPER, Christa , LORENZ, Patrick , ECK, Jürgen
IPC分类号： C12N15/10
CPC分类号： C12N15/1003
摘要： The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.
摘要翻译：本发明涉及用于分离和/或纯化核酸分子的装置，所述核酸分子适于结合和/或失活用于DNA操作的试剂或酶的活性的抑制剂并分离多个的核酸分子的大小。此外，本发明涉及用于分离核酸分子的方法，其包括将样品应用于本发明的装置，其中所述核酸分子优选代表给定栖息地的宏基因组的一部分。此外，本发明涉及产生至少一种基因文库的方法，所述基因文库包含通过本发明方法分离的核酸分子和通过本发明方法分离的核酸分子。

5. 发明申请

WO2011101457A1 CHIMERIC SURFACE ACTIVE PROTEINS 审中-公开
标题翻译： CHIMERIC表面活性蛋白
公开(公告)号：WO2011101457A1
公开(公告)日：2011-08-25
申请号：PCT/EP2011/052470
申请日：2011-02-18
申请人： B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG , MEURER, Guido , GABOR, Esther , BACHERT, Anke , ECK, Jürgen
发明人： MEURER, Guido , GABOR, Esther , BACHERT, Anke , ECK, Jürgen
IPC分类号： C07K14/37 , A61K47/42 , C09D189/00
CPC分类号： C07K14/37 , A61K9/286 , A61K9/2873 , C09D189/00 , C12N15/62
摘要： The present invention relates to a nucleic acid molecule encoding a chimeric protein having the biochemical activity of a surface active protein, wherein said chimeric protein comprises: (a) an N-terminal portion of a first surface active protein, wherein the N-terminal portion is devoid of between 0 and 10 of the most N-terminal amino acids of the mature first surface active protein; and, C-terminally thereof, (b) a C-terminal portion of a second surface active protein, wherein the C-terminal portion is devoid of between 0 and 10 of the most C-terminal amino acids of the mature second surface active protein. The present invention further relates to a vector, a non-human host and a method for the production of a chimeric protein having the biochemical activity of a surface active protein. In addition, the present invention relates to a chimeric protein encoded by the nucleic acid molecule of the invention and a composition comprising the chimeric protein. The chimeric protein may only consist of the above mentioned core of (a) and (b), but may also be flanked by additional components of the core, i.e. (a) or (b) or by (an) additional complete core(s) (a) and (b). The present invention furthermore relates to a method of coating and/or impregnating a material, comprising contacting the material with the chimeric protein or the composition of the invention.
摘要翻译：本发明涉及编码具有表面活性蛋白的生化活性的嵌合蛋白的核酸分子，其中所述嵌合蛋白包含：（a）第一表面活性蛋白的N末端部分，其中所述N末端部分没有成熟的第一表面活性蛋白的最多N末端氨基酸的0和10之间; 并且其C末端，（b）第二表面活性蛋白质的C末端部分，其中所述C末端部分缺少所述成熟第二表面活性蛋白质的最C末端氨基酸的0至10个。本发明还涉及载体，非人宿主和生产具有表面活性蛋白的生化活性的嵌合蛋白的方法。此外，本发明涉及由本发明的核酸分子编码的嵌合蛋白质和包含该嵌合蛋白质的组合物。嵌合蛋白质只能由上述（a）和（b）的核心组成，但也可以由核心的另外的组分，即（a）或（b）或另外一个完整的核心）（a）和（b）。本发明还涉及一种涂覆和/或浸渍材料的方法，包括使材料与本发明的嵌合蛋白或组合物接触。

6. 发明申请

WO2012172050A1 NEW MEANS AND METHODS FOR PRODUCING PROPANEDIOL 审中-公开
标题翻译：用于生产丙二醇的新手段和方法
公开(公告)号：WO2012172050A1
公开(公告)日：2012-12-20
申请号：PCT/EP2012/061449
申请日：2012-06-15
申请人： B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG , KOCH, Daniel , MEURER, Guido , ECK, Jürgen
发明人： KOCH, Daniel , MEURER, Guido , ECK, Jürgen
IPC分类号： C12P7/18 , C12P7/24 , C12N9/00 , C12N9/02 , C12N9/10 , C12N15/52 , C12N1/21
CPC分类号： C12P7/24 , C12N9/0006 , C12N9/0008 , C12N9/13 , C12P7/18 , C12Y101/01077 , C12Y102/01 , C12Y208/03001 , Y02P20/52
摘要： The present invention relates to a host cell having an elevated expression or activity of an enzyme as compared with the parent cell from which it has been derived, said enzyme having lactoyl-CoA reductase activity. Furthermore, provided is a method of producing lactaldehyde and/or 1,2-propanediol, said method comprising culturing said host cell and/or utilizing said enzyme to produce said compound.
摘要翻译：本发明涉及一种具有升高的酶表达或活性的宿主细胞，与其衍生的亲本细胞相比，所述酶具有乳酰基-CoA还原酶活性。此外，提供了生产乳醛和/或1,2-丙二醇的方法，所述方法包括培养所述宿主细胞和/或利用所述酶产生所述化合物。

7. 发明申请

WO2012072777A1 NOVEL CATALASES 审中-公开
标题翻译：新品推荐
公开(公告)号：WO2012072777A1
公开(公告)日：2012-06-07
申请号：PCT/EP2011/071576
申请日：2011-12-01
申请人： B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG , SCHULZE, Renate , ECK, Jürgen
发明人： SCHULZE, Renate , ECK, Jürgen
IPC分类号： A61K8/66 , A61K38/44 , A61L12/08 , A61Q19/08 , C11D3/386 , C12N9/08
CPC分类号： C12N9/0065 , A61K8/606 , A61K8/66 , A61K38/00 , A61Q5/10 , A61Q19/08 , C07K2319/00 , C07K2319/02 , C07K2319/21 , C07K2319/22 , C07K2319/23 , C07K2319/24 , C07K2319/41 , C07K2319/42 , C07K2319/43 , C07K2319/60 , C12Y111/01006 , D06L4/40 , D21C5/005 , D21H17/005
摘要： The invention relates to a nucleic acid molecule encoding a polypeptide having the activity of a catalase (EC 1.1 1.1.6), which nucleic acid molecule is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule comprising or consisting of a nucleic acid molecule encoding a polypeptide having the activity of a catalase the amino acid sequence of which is at least 99% identical to the amino acid sequence of SEQ ID NO: 1, (d) a nucleic acid molecule encoding a polypeptide having the activity of a catalase and comprising or consisting of a nucleotide sequence which is at least 98% identical to the nucleotide sequence of SEQ ID NO: 2, (e) a fragment of the polypeptide of any of (a) to (d) having the activity of a catalase and comprising at least 675 amino acids; or (f) the nucleic acid sequence of any of (a) to (d) wherein T is U.
摘要翻译：本发明涉及编码具有过氧化氢酶活性的多肽的核酸分子（EC 1.1 1.1.6），该核酸分子是（a）编码包含SEQ ID NO：1所示氨基酸序列或由SEQ ID NO： ID NO：1; （b）包含SEQ ID NO：2的核苷酸序列或由其组成的核酸分子; （c）核酸分子，其包含编码具有与SEQ ID NO：1的氨基酸序列至少99％相同的氨基酸序列的过氧化氢酶活性的多肽的核酸分子，（d ）编码具有过氧化氢酶活性并且由与SEQ ID NO：2的核苷酸序列至少98％相同的核苷酸序列组成或由其组成的核酸分子，（e）任何（a）至（d）具有过氧化氢酶的活性并且包含至少675个氨基酸; 或（f）（a）至（d）中任一项的核酸序列，其中T为U.

8. 发明申请

WO2011036174A1 A NOVEL METHOD FOR THE PRODUCTION OF A ANTIMICROBIAL PEPTIDE 审中-公开
标题翻译：一种生产抗微生物肽的新方法
公开(公告)号：WO2011036174A1
公开(公告)日：2011-03-31
申请号：PCT/EP2010/063971
申请日：2010-09-22
申请人： B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG , NAUMER, Christian , KROHN, Michael , TIFFERT, Yvonne , ECK, Jürgen
发明人： NAUMER, Christian , KROHN, Michael , TIFFERT, Yvonne , ECK, Jürgen
IPC分类号： A61K38/17 , C07K14/47 , C12P21/00
CPC分类号： C07K14/4703 , A61K38/4873 , C12P21/06 , Y02A50/401 , A61K2300/00
摘要： The present invention relates to a method of producing a peptide consisting of the amino acids 63 to 110 of dermcidin (SEQ ID NO: 3) comprising (a) culturing a host cell carrying a nucleic acid molecule encoding the peptide in an expressible form, and (b) optionally isolating the peptide from the culture. Furthermore, the invention relates to a nucleic acid molecule encoding a fusion protein comprising or consisting of (a) a peptide heterologous with regard to dermcidin protein-tag; and, C-terminally thereof (b) a peptide having the antimicrobial activity of dermcidin wherein the fusion protein contains an arginine residue located immediately N-terminally of the peptide of (b).
摘要翻译：本发明涉及一种由皮霉素（SEQ ID NO：3）的氨基酸63至110组成的肽的方法，该方法包括：（a）培养携带编码可表达形式的肽的核酸分子的宿主细胞，以及（b）任选地从培养物中分离肽。此外，本发明涉及编码融合蛋白的核酸分子，其包含或由（a）针对皮肤素蛋白标签异源的肽组成或由其组成; 及其C-末端（b）具有抗皮肤杀菌活性的肽，其中所述融合蛋白含有位于（b）的肽的N-末端的精氨酸残基。

9. 发明授权

EP2536744B1 CHIMERIC SURFACE ACTIVE PROTEINS 有权
公开(公告)号：EP2536744B1
公开(公告)日：2018-10-17
申请号：EP11706789.2
申请日：2011-02-18
申请人： B.R.A.I.N. Biotechnology Research And Information Network AG
发明人： MEURER, Guido , GABOR, Esther , BACHERT, Anke , ECK, Jürgen
IPC分类号： C07K14/37 , C09D189/00 , A61K9/28
CPC分类号： C07K14/37 , A61K9/286 , A61K9/2873 , C09D189/00 , C12N15/62
摘要： The present invention relates to a nucleic acid molecule encoding a chimeric protein having the biochemical activity of a surface active protein, wherein said chimeric protein comprises: (a) an N-terminal portion of a first surface active protein, wherein the N-terminal portion is devoid of between 0 and 10 of the most N-terminal amino acids of the mature first surface active protein; and, C-terminally thereof, (b) a C-terminal portion of a second surface active protein, wherein the C-terminal portion is devoid of between 0 and 10 of the most C-terminal amino acids of the mature second surface active protein. The present invention further relates to a vector, a non-human host and a method for the production of a chimeric protein having the biochemical activity of a surface active protein. In addition, the present invention relates to a chimeric protein encoded by the nucleic acid molecule of the invention and a composition comprising the chimeric protein. The chimeric protein may only consist of the above mentioned core of (a) and (b), but may also be flanked by additional components of the core, i.e. (a) or (b) or by (an) additional complete core(s) (a) and (b). The present invention furthermore relates to a method of coating and/or impregnating a material, comprising contacting the material with the chimeric protein or the composition of the invention.

10. 发明授权

EP1730300B1 NITRILE HYDRATASES FROM METAGENOME LIBRARIES 有权
标题翻译：丁腈关宏基因组文库
公开(公告)号：EP1730300B1
公开(公告)日：2013-07-17
申请号：EP05715932.9
申请日：2005-03-10
申请人： B.R.A.I.N. Biotechnology Research And Information Network AG
发明人： VERSECK, Stefan , LIEBETON, Klaus , ECK, Jürgen
IPC分类号： C12Q1/68 , C12N9/88 , C12N15/15
CPC分类号： C12N9/88 , C12Y402/01084

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