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1. 发明申请

WO0109357A3 ETHANOL INDUCIBLE EXPRESSION SYSTEM 审中-公开
标题翻译：乙醇诱导表达系统
公开(公告)号：WO0109357A3
公开(公告)日：2001-10-11
申请号：PCT/GB0002750
申请日：2000-07-18
申请人： SYNGENTA LTD , JEPSON IAN , GREENLAND ANDREW JAMES , CADDICK MARK XAVIER , TOMSETT ARTHUR BRIAN
发明人： JEPSON IAN , GREENLAND ANDREW JAMES , CADDICK MARK XAVIER , TOMSETT ARTHUR BRIAN
IPC分类号： C12N15/82 , A01H5/00 , C12N5/10
CPC分类号： C12N15/8238
摘要： A chemically-inducible plant gene expression cassette is described. The expression cassette comprises a first promoter operatively linked to the alcR regulator protein sequence obtainable from Aspergillus nidulans which encodes the AlcR regulator protein, and an inducible promoter obtainable from the alcA gene promoter of Aspergillus nidulans operatively linked to a target gene, the inducible promoter being activated by the regulator protein in the presence of an effective exogenous inducer whereby application of the inducer causes expression of the target gene and wherein the alcA promoter has three or more binding sites for the AlcR protein. According to a preferred embodiment of the invention, the promoter comprises 4 direct repeat sequences for the AlcR protein.
摘要翻译：描述了化学诱导型植物基因表达盒。表达盒包含可操作地连接到可从编码AlcR调节蛋白的构巢曲霉实现的调节蛋白序列的第一启动子，以及可从第i 与靶基因可操作地连接的构巢曲霉的alcA基因启动子，诱导型启动子在有效的外源性诱导物存在下由调节蛋白激活，由此施用诱导物引起表达靶基因，其中所述alcA启动子具有三个或更多个AlcR蛋白的结合位点。根据本发明的一个优选实施方案，启动子包含4个用于AlcR蛋白的直接重复序列。

2. 发明申请

WO1993021334A1 DNA CONSTRUCTS AND PLANTS INCORPORATING THEM 审中-公开
标题翻译： DNA构建与植物鉴定
公开(公告)号：WO1993021334A1
公开(公告)日：1993-10-28
申请号：PCT/GB1993000764
申请日：1993-04-13
申请人： ZENECA LIMITED , CADDICK, Mark, Xavier , GREENLAND, Andrew, James , RIDDELL, Kay, Victoria , SCHUCH, Wolfgang, Walter , TOMSETT, Arthur, Brian
发明人： ZENECA LIMITED
IPC分类号： C12N15/82
CPC分类号： C12N15/8237 , C12N15/8238
摘要： The invention relates to a chemically-inducible plant gene expression cassette and to plant cells transformed therewith. The expression cassette is suitable for use in any dicothyledonous or monocotyledonous plant. It comprises a first promoter operatively linked to a regulator sequence which encodes a regulator protein, and an inducible promoter operatively linked to a target gene, the inducible promoter being activated by the regulator protein in the presence of an effective exogenous inducer whereby application of the inducer causes expression of the target gene. The regulator sequence may be derived from the alcR gene, and the inducible promoter may be derived from the alcA gene promoter (both obtainable from Aspergillus nidulans) A chimeric promoter comprising an upstream regulatory region and a heterologous downstream transcription initiation region is also disclosed. The regulatory sequence may be derived from the alcA gene promoter.
摘要翻译：本发明涉及一种化学诱导型植物基因表达盒，并涉及用其转化的植物细胞。表达盒适用于任何双子叶植物或单子叶植物。其包含可操作地连接到编码调节蛋白的调节子序列的第一启动子和可操作地连接到靶基因的诱导型启动子，所述诱导型启动子在有效的外源诱导物存在下由调节蛋白激活，从而施用诱导物导致靶基因的表达。调节序列可以衍生自alcR基因，诱导型启动子可以衍生自alcA基因启动子（均可从构巢曲霉获得）。还公开了包含上游调节区和异源下游转录起始区的嵌合启动子。调节序列可以来源于alcA基因启动子。

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