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1. 发明申请

WO2008103643A1 INVERTEBRATE MICRORNAS 审中-公开
标题翻译：反相微阵列
公开(公告)号：WO2008103643A1
公开(公告)日：2008-08-28
申请号：PCT/US2008/054251
申请日：2008-02-19
申请人： MONSANTO TECHNOLOGY, LLC , ALLEN, Edwards , DONOVAN, William, P. , HECK, Gregory, R. , ROBERT, James, K. , URSIN, Virginia , ZHANG, Yuanji
发明人： ALLEN, Edwards , DONOVAN, William, P. , HECK, Gregory, R. , ROBERT, James, K. , URSIN, Virginia , ZHANG, Yuanji
IPC分类号： C12N15/29
CPC分类号： C12N15/8286 , A01N63/02 , C12N15/113 , C12N15/8285 , C12N2310/14 , C12N2310/141 , C12N2310/531 , Y02A40/162 , Y02A40/164
摘要： This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non- natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.
摘要翻译：本发明提供具有抗无脊椎害虫的植物。更具体地，本发明公开了一种非天然转基因植物细胞，其在植物中表达至少一种无脊椎动物miRNA，用于抑制无脊椎害虫或与无脊椎害虫相关的共生体的靶基因。还提供了用于在植物中表达至少一种无脊椎动物miRNA的重组DNA构建体，包含本发明的非天然转基因植物细胞的非天然转基因植物，从非天然转基因植物细胞生长的非天然转基因植物以及由非天然转基因植物产生的非天然转基因种子，以及由本发明的非天然转基因植物细胞，植物或种子产生的商品。本发明还提供一种抑制植物无脊椎动物害虫或与无脊椎动物相关共生体的至少一种靶基因的方法，包括提供包含本发明非天然转基因植物细胞的植物，其中无脊椎动物是无脊椎动物害虫，重组DNA在非天然转基因植物细胞中转录至重组miRNA前体，当无脊椎害虫摄入重组miRNA前体时，抑制至少一种靶基因。

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