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1. 发明申请

WO2013003178A1 SULFATION OF WNT PATHWAY PROTEINS 审中-公开
标题翻译： WNT途径蛋白的硫化
公开(公告)号：WO2013003178A1
公开(公告)日：2013-01-03
申请号：PCT/US2012/043451
申请日：2012-06-21
申请人： ENZO BIOCHEM, INC. , RABBANI, Joshua , DONEGAN, James J. , LI, Xiaofeng
发明人： RABBANI, Joshua , DONEGAN, James J. , LI, Xiaofeng
IPC分类号： C07K14/51 , C07K7/04
CPC分类号： A61K38/1709 , A61K38/08 , A61K38/10 , C07K14/47 , C07K14/51 , C07K14/705 , C07K14/71
摘要： Provided is a composition comprising a peptide comprising amino acids and/or amino acid analogs comprising a continuous sequence of a sclerostin fragment comprising Tyr43 or Tyr213. Also provided is a composition comprising a peptide comprising less than about 75 amino acids and/or amino acid analogs including an amino acid or amino acid analog capable of being sulfated, where the composition is capable of inhibiting sclerostin binding to an LRP. Further provided is a composition comprising a peptide comprising less than about 75 amino acids and/or amino acid analogs including an amino acid or amino acid analog capable of being post-translationally sulfated, where the composition is capable of inhibiting binding of a protein ligand comprising a sulfation site to its binding partner. Additionally provided is a method of enhancing a Wnt signaling pathway comprising contacting an LRP5/6 receptor in the Wnt signaling pathway with either of the above-described compositions that comprise a sequence of a sclerostin fragment or is capable of inhibiting sclerostin binding to an LRP, where the tyrosine or tyrosine analog is not sulfated, in a manner sufficient to enhance the Wnt signaling pathway. Further provided is a method of treating a subject having a disease exacerbated by inhibition of a Wnt signaling pathway comprising administering either of the above-described compositions that comprise a sequence of a sclerostin fragment or is capable of inhibiting sclerostin binding to an LRP, where the tyrosine or tyrosine analog is not sulfated, to the subject in a manner sufficient to reduce the inhibition of the Wnt signaling pathway. Also, a method of inhibiting binding of a protein ligand comprising a sulfation site to its binding partner is provided. The method comprises adding the above-described composition that is capable of inhibiting binding of a protein ligand to its binding partner to the protein ligand and its binding partner in a manner sufficient to inhibit binding of the protein ligand to its binding partner.
摘要翻译：提供了包含包含氨基酸和/或氨基酸类似物的肽的组合物，其包含包含Tyr43或Tyr213的硬化蛋白片段的连续序列。还提供了包含包含少于约75个氨基酸和/或氨基酸类似物的肽的组合物，其包含能够被硫酸化的氨基酸或氨基酸类似物，其中该组合物能够抑制与LRP结合的硬皮蛋白。还提供了一种组合物，其包含包含小于约75个氨基酸和/或氨基酸类似物的肽，其包含能够翻译后硫酸化的氨基酸或氨基酸类似物，其中组合物能够抑制蛋白质配体的结合，所述蛋白质配体包含硫酸化位点到其结合配偶体。另外提供了增强Wnt信号传导途径的方法，包括使Wnt信号传导途径中的LRP5 / 6受体与包含硬化蛋白片段序列的任一上述组合物接触或能够抑制硬化蛋白酶与LRP结合，其中酪氨酸或酪氨酸类似物不被硫酸化，以足以增强Wnt信号通路的方式。还提供了一种治疗具有通过抑制Wnt信号传导途径加重疾病的受试者的方法，包括施用包含硬化蛋白酶片段序列的上述组合物或能够抑制硬化蛋白与LRP的结合，其中酪氨酸或酪氨酸类似物不以足以降低Wnt信号传导途径抑制的方式硫酸化。此外，提供了抑制包含硫酸化位点的蛋白质配体与其结合配偶体的结合的方法。该方法包括加入能够以足以抑制蛋白质配体与其结合配偶体的结合的方式将蛋白质配体与其结合配偶体结合至蛋白质配体及其结合配偶体的上述组合物。

2. 发明申请

WO2011156252A3 SULFATION OF WNT PATHWAY PROTEINS 审中-公开
标题翻译： WNT途径蛋白质的硫化
公开(公告)号：WO2011156252A3
公开(公告)日：2012-02-02
申请号：PCT/US2011039223
申请日：2011-06-06
申请人： ENZO BIOCHEM INC , RABBANI JOSHUA , DONEGAN JAMES J
发明人： RABBANI JOSHUA , DONEGAN JAMES J
IPC分类号： C07K14/51 , C07K14/47 , C07K14/705 , C07K14/71 , C07K16/18 , C07K16/22 , C07K16/28
CPC分类号： C07K16/22 , C07K14/47 , C07K14/51 , C07K14/705 , C07K14/71 , C07K2317/54 , C07K2317/55 , C07K2317/76
摘要： Provided is a protein comprising an antibody binding site that binds to a sulfated epitope of a Wnt pathway protein that is not Wnt5A, Wnt11, or Wnt3a. Also provided is a composition comprising an isolated, and purified Wnt pathway protein, where the protein is sulfated but not glycosylated. Additionally provided is a preparation of a Wnt pathway protein comprising at least one sulfation site and at least one glycosylation site, where all of the Wnt pathway protein in the preparation is glycosylated but not sulfated. Further provided is a composition comprising a peptide less than 75 amino acids or amino acid analogs, the peptide consisting of a fragment of a Wnt pathway protein, wherein the fragment is sulfated. A modified Wnt pathway protein comprising a sulfation site that, is not present, in the native Wnt pathway protein is also provided. Also provided is a method of detecting or quantifying a sulfated Wnt pathway protein in a preparation. Additionally, a modified Wnt pathway protein, lacking a sulfation site that, is present in the native Wnt pathway protein is provided. Also provided, is methods of treating a subject having a disease exacerbated by Wnt activation. Additionally, a method of treating a subject having a disease exacerbated by Wnt inhibition is provided.
摘要翻译：提供了包含结合不是Wnt5A，Wnt11或Wnt3a的Wnt途径蛋白的硫酸化表位的抗体结合位点的蛋白质。还提供了包含分离的和纯化的Wnt途径蛋白质的组合物，其中蛋白质被硫酸化但不被糖基化。另外提供了包含至少一个硫酸化位点和至少一个糖基化位点的Wnt途径蛋白质的制剂，其中所述制剂中的所有Wnt途径蛋白质被糖基化但不硫酸化。还提供了包含小于75个氨基酸或氨基酸类似物的肽的组合物，所述肽由Wnt途径蛋白质的片段组成，其中所述片段被硫酸化。还提供了包含未存在于天然Wnt途径蛋白中的硫酸化位点的修饰的Wnt途径蛋白。还提供了在制剂中检测或定量硫酸化Wnt途径蛋白的方法。另外，提供了在天然Wnt途径蛋白中存在缺乏硫酸化位点的修饰的Wnt途径蛋白。还提供了治疗患有由Wnt活化加重的疾病的受试者的方法。另外，提供了治疗由Wnt抑制加剧的疾病的受试者的方法。

3. 发明申请

WO2012027195A1 ASSAYS FOR DETECTING MODIFIED COMPOUNDS 审中-公开
标题翻译：用于检测改性化合物的测定
公开(公告)号：WO2012027195A1
公开(公告)日：2012-03-01
申请号：PCT/US2011/048243
申请日：2011-08-18
申请人： ENZO BIOCHEM, INC. , RABBANI, Elazar , RABBANI, Joshua , PANDE, Praveen , STAVRIANOPOULOS, Jannis, G.
发明人： RABBANI, Elazar , RABBANI, Joshua , PANDE, Praveen , STAVRIANOPOULOS, Jannis, G.
IPC分类号： C12Q1/48 , C12Q1/527 , G01N33/58 , G01N33/542
CPC分类号： G01N33/6803 , C12Q1/485 , C12Y207/01091 , C12Y207/11001 , G01N33/92 , G01N2405/08 , G01N2410/00 , G01N2440/14 , G01N2440/30
摘要： Provided are methods and compositions which are useful for separating, isolating, detecting, and quantifying compounds of interest which have been modified chemically, enzymatically or catalytically from other compounds which have not been so modified. The modifications may take the form of functional groups which are gained, lost or retained by the compounds of interest.
摘要翻译：提供的方法和组合物可用于分离，分离，检测和定量已经由尚未被这样修饰的其它化合物化学，酶促或催化改性的化合物。这些修饰可以采取由感兴趣的化合物获得，丢失或保留的官能团的形式。

4. 发明申请

WO2011156252A2 SULFATION OF WNT PATHWAY PROTEINS 审中-公开
标题翻译： WNT途径蛋白的硫化
公开(公告)号：WO2011156252A2
公开(公告)日：2011-12-15
申请号：PCT/US2011/039223
申请日：2011-06-06
申请人： ENZO BIOCHEM. INC. , RABBANI, Joshua , DONEGAN, James, J.
发明人： RABBANI, Joshua , DONEGAN, James, J.
IPC分类号： C07K14/51 , C07K16/22 , C07K16/18 , C07K14/47 , C07K14/71 , C07K16/28 , C07K14/705
CPC分类号： C07K16/22 , C07K14/47 , C07K14/51 , C07K14/705 , C07K14/71 , C07K2317/54 , C07K2317/55 , C07K2317/76
摘要： Provided is a protein comprising an antibody binding site that binds to a sulfated epitope of a Wnt pathway protein that is not Wnt5A, Wnt11, or Wnt3a. Also provided is a composition comprising an isolated, and purified Wnt pathway protein, where the protein is sulfated but not glycosylated. Additionally provided is a preparation of a Wnt pathway protein comprising at least one sulfation site and at least one glycosylation site, where all of the Wnt pathway protein in the preparation is glycosylated but not sulfated. Further provided is a composition comprising a peptide less than 75 amino acids or amino acid analogs, the peptide consisting of a fragment of a Wnt pathway protein, wherein the fragment is sulfated. A modified Wnt pathway protein comprising a sulfation site that, is not present, in the native Wnt pathway protein is also provided. Also provided is a method of detecting or quantifying a sulfated Wnt pathway protein in a preparation. Additionally, a modified Wnt pathway protein, lacking a sulfation site that, is present in the native Wnt pathway protein is provided. Also provided, is methods of treating a subject having a disease exacerbated by Wnt activation. Additionally, a method of treating a subject having a disease exacerbated by Wnt inhibition is provided.
摘要翻译：提供了包含结合非Wnt5A，Wnt11或Wnt3a的Wnt途径蛋白的硫酸化表位的抗体结合位点的蛋白质。还提供了包含分离的和纯化的Wnt途径蛋白质的组合物，其中蛋白质被硫酸化但未被糖基化。另外提供了包含至少一个硫酸化位点和至少一个糖基化位点的Wnt途径蛋白质的制剂，其中所述制剂中的所有Wnt途径蛋白质被糖基化但不被硫酸化。还提供了包含少于75个氨基酸或氨基酸类似物的肽的组合物，所述肽由Wnt途径蛋白质的片段组成，其中所述片段被硫酸化。还提供了包含不存在于天然Wnt途径蛋白中的硫酸化位点的修饰的Wnt途径蛋白。还提供了在制剂中检测或定量硫酸化Wnt途径蛋白的方法。另外，提供了一种修饰的Wnt途径蛋白，其缺乏存在于天然Wnt途径蛋白中的硫酸化位点。还提供了治疗患有由Wnt活化加剧的疾病的受试者的方法。另外，提供了治疗患有由Wnt抑制加剧的疾病的受试者的方法。

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