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1. 发明授权

US08852948B2 Colorimetric protein analysis method 有权
标题翻译：比色蛋白分析法
公开(公告)号：US08852948B2
公开(公告)日：2014-10-07
申请号：US12185575
申请日：2008-08-04
申请人： Michael J. Collins, Sr. , Joseph J. Lambert , Timothy A. Zawatsky , David L. Herman
发明人： Michael J. Collins, Sr. , Joseph J. Lambert , Timothy A. Zawatsky , David L. Herman
IPC分类号： G01N33/00 , G01N21/00 , G01N33/68 , G01N33/52
CPC分类号： G01N33/6803 , G01N33/52
摘要： A protein analysis instrument and method are disclosed. The method includes mixing a binding dye composition and a protein sample using a homogenizer, measuring a parameter selected from the group consisting of the speed of the homogenizer and the resistance of the mixture to the homogenizer, adjusting the speed of the homogenizer based upon the measured parameter, pumping unreacted dye composition from the mixture and to a calorimeter, and measuring the absorbance of the dye composition in the calorimeter. Aspects of the invention also include inserting a spout into a sample cup at a position where the spout opening is positioned to avoid foam and precipitate generated by the mixing step and above the bottom of the sample cup and thereafter pumping the dye composition from the mixture in the sample cup through the spout and to a colorimeter.
摘要翻译：公开了一种蛋白质分析仪器和方法。该方法包括使用均化器混合结合染料组合物和蛋白质样品，测量选自均化器的速度和混合物对均化器的电阻的参数，基于测量的调节匀浆器的速度参数，从混合物和比色计中抽出未反应的染料组合物，并测量色度计中染料组合物的吸光度。本发明的方面还包括将喷口插入样品杯中，在出口开口的位置处，以避免由混合步骤产生的泡沫和沉淀，并且在样品杯的底部之上，然后从混合物中抽出染料组合物样品杯通过喷口和色度计。

2. 发明授权

US07997790B2 Dual energy source loss-on-drying instrument 有权
标题翻译：双能量损耗干燥仪
公开(公告)号：US07997790B2
公开(公告)日：2011-08-16
申请号：US12508648
申请日：2009-07-24
申请人： Robert N. Revesz , Michael J. Collins, Sr.
发明人： Robert N. Revesz , Michael J. Collins, Sr.
IPC分类号： G01N25/00 , G01J5/00
CPC分类号： G01N5/045 , G01N1/44
摘要： An instrument and associated method are disclosed for the loss-on-drying determination of the volatile content of a wide variety of samples. The instrument includes a cavity in which a sample for which the volatile content is to be determined can be placed, a first source for introducing microwaves into the cavity that have frequencies substantially other than infrared frequencies, a second source for introducing radiant heat into the cavity at frequencies different from the frequencies introduced by the first source, an analytical balance for measuring the weight of a sample while the sample is in the cavity and on the balance, a temperature sensor capable of measuring and positioned to measure the temperature of a sample in the cavity and on the balance, and a processor in communication with the temperature sensor and each of the first and second sources for controlling the introduction of the frequencies of microwave and radiant energy into the cavity in response to the temperatures measured by the temperature sensor to control the sample temperature until the microwaves from the first source and the radiant heat from the second source dry the sample sufficiently for the processor to determine the volatile content of the sample based on the weight change of the sample on the balance.
摘要翻译：公开了用于各种样品的挥发物含量的干燥损失测定的仪器和相关方法。该仪器包括其中可以放置要确定挥发物含量的样品的空腔，用于将微波引入具有基本上不同于红外频率的频率的空腔的第一源，用于将辐射热引入腔中的第二源在与第一源引入的频率不同的频率处，用于测量样品在腔中和平衡时的重量的分析天平，能够测量和定位以测量样品的温度的温度传感器空腔和平衡部，以及与温度传感器和第一和第二源中的每一个通信的处理器，用于响应于由温度传感器测量的温度而将微波和辐射能的频率引入空腔中控制样品温度，直到来自第一个来源的微波和来自th的辐射热第二来源充分干燥样品以使处理器基于样品在天平上的重量变化来确定样品的挥发性含量。

3. 发明申请

US20100291686A1 AUTOMATED PROTEIN ANALYZER 失效
标题翻译：自动蛋白分析仪
公开(公告)号：US20100291686A1
公开(公告)日：2010-11-18
申请号：US12721688
申请日：2010-03-11
申请人： Michael J. Collins, SR. , Joseph J. Lambert , Timothy A. Zawatsky , David L. Herman
发明人： Michael J. Collins, SR. , Joseph J. Lambert , Timothy A. Zawatsky , David L. Herman
IPC分类号： G01N31/00 , G01N33/00
CPC分类号： G01N33/6839 , G01N1/286 , G01N21/17 , G01N21/251 , G01N33/12 , G01N33/6803 , G01N35/1095 , G01N2001/4088 , Y10T436/10 , Y10T436/105831 , Y10T436/25
摘要： A dye binding method for protein analysis is disclosed. The method includes the steps of preparing an initial reference dye solution of unknown concentration from an initial reference dye concentrate and creating an electronic signal based upon the absorbance of the initial reference dye solution. Thereafter, an electronic signal is created based upon the absorbance of a dye filtrate solution prepared from the initial reference dye solution and an initial protein sample. The absorbance signals from the reference dye solution and the dye filtrate solution are sent to a processor that compares the respective absorbances and calculates the protein content of the protein sample based upon the difference between the absorbances. An electronic signal is created based upon the absorbance of a successive dye filtrate solution prepared from the reference dye solution and a successive protein sample, and the absorbance signal from the successive sample dye filtrate solution is sent to the processor to calculate the protein content of the successive sample based upon the difference between the absorbance of the initial reference dye solution and the absorbance of the successive dye filtrate solution.
摘要翻译：公开了用于蛋白质分析的染料结合方法。该方法包括以下步骤：从初始参考染料浓缩物制备未知浓度的初始参考染料溶液，并基于初始参考染料溶液的吸光度产生电子信号。此后，基于由初始参考染料溶液和初始蛋白质样品制备的染料滤液的吸光度，产生电子信号。将来自参考染料溶液和染料滤液的吸光度信号送至处理器，该处理器根据吸光度之差来比较各吸光度，并计算蛋白质样品的蛋白质含量。基于由参考染料溶液和连续蛋白质样品制备的连续染料滤液的吸光度产生电子信号，并将来自连续样品染料滤液的溶液的吸光度信号发送到处理器，以计算蛋白质含量基于初始参考染料溶液的吸光度与连续的染料滤液的吸光度之间的差异的连续样品。

4. 发明授权

US08663993B2 Automated protein analyzer 失效
标题翻译：自动蛋白分析仪
公开(公告)号：US08663993B2
公开(公告)日：2014-03-04
申请号：US12721688
申请日：2010-03-11
申请人： Michael J Collins, Sr. , Joseph J Lambert , Timothy A Zawatsky , David L Herman
发明人： Michael J Collins, Sr. , Joseph J Lambert , Timothy A Zawatsky , David L Herman
IPC分类号： G01N33/00
CPC分类号： G01N33/6839 , G01N1/286 , G01N21/17 , G01N21/251 , G01N33/12 , G01N33/6803 , G01N35/1095 , G01N2001/4088 , Y10T436/10 , Y10T436/105831 , Y10T436/25
摘要： A dye binding method for protein analysis is disclosed. The method includes the steps of preparing an initial reference dye solution of unknown concentration from an initial reference dye concentrate and creating an electronic signal based upon the absorbance of the initial reference dye solution. Thereafter, an electronic signal is created based upon the absorbance of a dye filtrate solution prepared from the initial reference dye solution and an initial protein sample. The absorbance signals from the reference dye solution and the dye filtrate solution are sent to a processor that compares the respective absorbances and calculates the protein content of the protein sample based upon the difference between the absorbances. An electronic signal is created based upon the absorbance of a successive dye filtrate solution prepared from the reference dye solution and a successive protein sample, and the absorbance signal from the successive sample dye filtrate solution is sent to the processor to calculate the protein content of the successive sample based upon the difference between the absorbance of the initial reference dye solution and the absorbance of the successive dye filtrate solution.
摘要翻译：公开了用于蛋白质分析的染料结合方法。该方法包括以下步骤：从初始参考染料浓缩物制备未知浓度的初始参考染料溶液，并基于初始参考染料溶液的吸光度产生电子信号。此后，基于由初始参考染料溶液和初始蛋白质样品制备的染料滤液的吸光度，产生电子信号。将来自参考染料溶液和染料滤液的吸光度信号送至处理器，该处理器根据吸光度之差来比较各吸光度，并计算蛋白质样品的蛋白质含量。基于由参考染料溶液和连续蛋白质样品制备的连续染料滤液的吸光度产生电子信号，并将来自连续样品染料滤液的溶液的吸光度信号发送到处理器，以计算蛋白质含量基于初始参考染料溶液的吸光度与连续的染料滤液的吸光度之间的差异的连续样品。

5. 发明申请

US20100290949A1 AUTOMATED PROTEIN ANALYZER 有权
标题翻译：自动蛋白分析仪
公开(公告)号：US20100290949A1
公开(公告)日：2010-11-18
申请号：US12721993
申请日：2010-03-11
申请人： Michael J. Collins, SR. , Joseph J. Lambert , Timothy A. Zawatsky , David L. Herman
发明人： Michael J. Collins, SR. , Joseph J. Lambert , Timothy A. Zawatsky , David L. Herman
IPC分类号： B01J19/00
CPC分类号： G01N33/6839 , G01N1/286 , G01N21/17 , G01N21/251 , G01N33/12 , G01N33/6803 , G01N35/1095 , G01N2001/4088 , Y10T436/10 , Y10T436/105831 , Y10T436/25
摘要： A protein analysis kit is disclosed. The kit includes a sample cup for mixing a protein sample with a dye-binding solution and a filter holder for being positioned in the sample cup. The filter holder includes a filter media and a depending spout below the filter media that reaches bottom portions of the cup when the filter media is positioned above the cup.
摘要翻译：公开了蛋白质分析试剂盒。该试剂盒包括用于将蛋白质样品与染料结合溶液混合的样品杯和用于定位在样品杯中的过滤器保持器。当过滤介质位于杯子上方时，过滤器保持器包括过滤介质和在过滤介质下方到达杯的底部的垂直喷口。

6. 发明授权

US07581876B2 Dual energy source loss-on-drying instrument 有权
标题翻译：双能量损耗干燥仪
公开(公告)号：US07581876B2
公开(公告)日：2009-09-01
申请号：US11457798
申请日：2006-07-15
申请人： Robert N. Revesz , Michael J. Collins, Sr.
发明人： Robert N. Revesz , Michael J. Collins, Sr.
IPC分类号： G01N5/02 , G01N25/56 , G01J5/00 , H05B6/64 , G01K7/00 , G01K13/00 , G01G9/00
CPC分类号： G01N5/045 , G01N1/44
摘要： An instrument and associated method are disclosed for the loss-on-drying determination of the volatile content of a wide variety of samples. The instrument includes a cavity in which a sample for which the volatile content is to be determined can be placed, a first source for introducing microwaves into the cavity that have frequencies substantially other than infrared frequencies, a second source for introducing radiant heat into the cavity at frequencies different from the frequencies introduced by the first source, an analytical balance for measuring the weight of a sample while the sample is in the cavity and on the balance, a temperature sensor capable of measuring and positioned to measure the temperature of a sample in the cavity and on the balance, and a processor in communication with the temperature sensor and each of the first and second sources for controlling the introduction of the frequencies of microwave and radiant energy into the cavity in response to the temperatures measured by the temperature sensor to control the sample temperature until the microwaves from the first source and the radiant heat from the second source dry the sample sufficiently for the processor to determine the volatile content of the sample based on the weight change of the sample on the balance.
摘要翻译：公开了用于各种样品的挥发物含量的干燥损失测定的仪器和相关方法。该仪器包括其中可以放置要确定挥发物含量的样品的空腔，用于将微波引入具有基本上不同于红外频率的频率的空腔的第一源，用于将辐射热引入腔中的第二源在与第一源引入的频率不同的频率处，用于测量样品在腔中和平衡时的重量的分析天平，能够测量和定位以测量样品的温度的温度传感器空腔和平衡部，以及与温度传感器和第一和第二源中的每一个通信的处理器，用于响应于由温度传感器测量的温度而将微波和辐射能的频率引入空腔中控制样品温度，直到来自第一个来源的微波和来自th的辐射热第二来源充分干燥样品以使处理器基于样品在天平上的重量变化来确定样品的挥发性含量。

7. 发明授权

US08147759B2 Automated protein analyzer 有权
标题翻译：自动蛋白分析仪
公开(公告)号：US08147759B2
公开(公告)日：2012-04-03
申请号：US11846598
申请日：2007-08-29
申请人： Michael J. Collins, Sr. , Joseph J. Lambert , Timothy A. Zawatsky , David L. Herman
发明人： Michael J. Collins, Sr. , Joseph J. Lambert , Timothy A. Zawatsky , David L. Herman
IPC分类号： G01N21/78
CPC分类号： G01N33/6839 , G01N1/286 , G01N21/17 , G01N21/251 , G01N33/12 , G01N33/6803 , G01N35/1095 , G01N2001/4088 , Y10T436/10 , Y10T436/105831 , Y10T436/25
摘要： A direct rapid automated protein analyzer is disclosed. The protein analyzer includes means for reducing protein samples to small particles, a reaction vessel in material transfer communication with the homogenizer, a reservoir for binding dye composition in fluid communication with the reaction vessel, a metering pump in fluid communication with the reservoir and the reaction vessel for distributing discrete predetermined amounts of a binding dye composition to the reaction vessel, a filter in fluid communication with the reaction vessel for separating solids from filtrate after a dye binding reaction has taken place in the reaction vessel, and a calorimeter in fluid communication with the filter and the reaction vessel for measuring the absorbance of the filtrate from the reaction vessel and the filter. The rapid analyzer can be used in conjunction with a kit that includes a sample cup for mixing a protein sample with a dye-binding solution and a filter holder for being positioned in the sample cup. The filter holder includes a filter media and a depending spout below the filter media that reaches bottom portions of the cup when the filter media is positioned above the cup. The kit can also include dye concentrate solution.
摘要翻译：公开了一种直接快速自动蛋白质分析仪。蛋白质分析仪包括将蛋白质样品还原成小颗粒的装置，与均化器材料转移连通的反应容器，用于结合与反应容器流体连通的染料组合物的储存器，与储存器流体连通的计量泵和反应用于将分散的预定量的结合染料组合物分配到反应容器中的过滤器，与反应容器流体连通的过滤器，用于在反应容器中发生染料结合反应后从滤液中分离固体;以及色度计，其与过滤器和用于测量来自反应容器和过滤器的滤液的吸光度的反应容器。快速分析仪可以与包括用于将蛋白质样品与染料结合溶液混合的样品杯和用于定位在样品杯中的过滤器保持器的试剂盒结合使用。当过滤介质位于杯子上方时，过滤器保持器包括过滤介质和在过滤介质下方到达杯的底部的垂直喷口。该试剂盒还可以包括染料浓缩液。

8. 发明申请

US20090061522A1 Automated Protein Analyzer 有权
标题翻译：自动蛋白分析仪
公开(公告)号：US20090061522A1
公开(公告)日：2009-03-05
申请号：US11846598
申请日：2007-08-29
申请人： Michael J. Collins, SR. , Joseph J. Lambert , Timothy A. Zawatsky , David L. Herman
发明人： Michael J. Collins, SR. , Joseph J. Lambert , Timothy A. Zawatsky , David L. Herman
IPC分类号： G01N33/02 , B01J19/00 , B01L3/00 , G01N33/68 , G01N21/01
CPC分类号： G01N33/6839 , G01N1/286 , G01N21/17 , G01N21/251 , G01N33/12 , G01N33/6803 , G01N35/1095 , G01N2001/4088 , Y10T436/10 , Y10T436/105831 , Y10T436/25
摘要： A direct rapid automated protein analyzer is disclosed. The protein analyzer includes means for reducing protein samples to small particles, a reaction vessel in material transfer communication with the homogenizer, a reservoir for binding dye composition in fluid communication with the reaction vessel, a metering pump in fluid communication with the reservoir and the reaction vessel for distributing discrete predetermined amounts of a binding dye composition to the reaction vessel, a filter in fluid communication with the reaction vessel for separating solids from filtrate after a dye binding reaction has taken place in the reaction vessel, and a calorimeter in fluid communication with the filter and the reaction vessel for measuring the absorbance of the filtrate from the reaction vessel and the filter. The rapid analyzer can be used in conjunction with a kit that includes a sample cup for mixing a protein sample with a dye-binding solution and a filter holder for being positioned in the sample cup. The filter holder includes a filter media and a depending spout below the filter media that reaches bottom portions of the cup when the filter media is positioned above the cup. The kit can also include dye concentrate solution.
摘要翻译：公开了一种直接快速自动蛋白质分析仪。蛋白质分析仪包括将蛋白质样品还原成小颗粒的装置，与均化器材料转移连通的反应容器，用于结合与反应容器流体连通的染料组合物的储存器，与储存器流体连通的计量泵和反应用于将分散的预定量的结合染料组合物分配到反应容器中的过滤器，与反应容器流体连通的过滤器，用于在反应容器中发生染料结合反应后从滤液中分离固体;以及色度计，其与过滤器和用于测量来自反应容器和过滤器的滤液的吸光度的反应容器。快速分析仪可以与包括用于将蛋白质样品与染料结合溶液混合的样品杯和用于定位在样品杯中的过滤器保持器的试剂盒结合使用。当过滤介质位于杯子上方时，过滤器保持器包括过滤介质和在过滤介质下方到达杯的底部的垂直喷口。该试剂盒还可以包括染料浓缩液。

9. 发明授权

US07968344B2 Automated protein analyzer 有权
标题翻译：自动蛋白分析仪
公开(公告)号：US07968344B2
公开(公告)日：2011-06-28
申请号：US12721993
申请日：2010-03-11
申请人： Michael J Collins, Sr. , Joseph J Lambert , Timothy A Zawatsky , David L Herman
发明人： Michael J Collins, Sr. , Joseph J Lambert , Timothy A Zawatsky , David L Herman
IPC分类号： G01N21/01 , G01N33/00 , B01L3/00
CPC分类号： G01N33/6839 , G01N1/286 , G01N21/17 , G01N21/251 , G01N33/12 , G01N33/6803 , G01N35/1095 , G01N2001/4088 , Y10T436/10 , Y10T436/105831 , Y10T436/25
摘要： A protein analysis kit is disclosed. The kit includes a sample cup for mixing a protein sample with a dye-binding solution and a filter holder for being positioned in the sample cup. The filter holder includes a filter media and a depending spout below the filter media that reaches bottom portions of the cup when the filter media is positioned above the cup.
摘要翻译：公开了蛋白质分析试剂盒。该试剂盒包括用于将蛋白质样品与染料结合溶液混合的样品杯和用于定位在样品杯中的过滤器保持器。当过滤介质位于杯子上方时，过滤器保持器包括过滤介质和在过滤介质下方到达杯的底部的垂直喷口。

10. 发明申请

US20090285257A1 Dual Energy Source Loss-on-Drying Instrument 有权
标题翻译：双能量干燥干燥仪
公开(公告)号：US20090285257A1
公开(公告)日：2009-11-19
申请号：US12508648
申请日：2009-07-24
申请人： Robert N. Revesz , Michael J. Collins, SR.
发明人： Robert N. Revesz , Michael J. Collins, SR.
IPC分类号： G01N25/00
CPC分类号： G01N5/045 , G01N1/44
摘要： An instrument and associated method are disclosed for the loss-on-drying determination of the volatile content of a wide variety of samples. The instrument includes a cavity in which a sample for which the volatile content is to be determined can be placed, a first source for introducing microwaves into the cavity that have frequencies substantially other than infrared frequencies, a second source for introducing radiant heat into the cavity at frequencies different from the frequencies introduced by the first source, an analytical balance for measuring the weight of a sample while the sample is in the cavity and on the balance, a temperature sensor capable of measuring and positioned to measure the temperature of a sample in the cavity and on the balance, and a processor in communication with the temperature sensor and each of the first and second sources for controlling the introduction of the frequencies of microwave and radiant energy into the cavity in response to the temperatures measured by the temperature sensor to control the sample temperature until the microwaves from the first source and the radiant heat from the second source dry the sample sufficiently for the processor to determine the volatile content of the sample based on the weight change of the sample on the balance.
摘要翻译：公开了用于各种样品的挥发物含量的干燥损失测定的仪器和相关方法。该仪器包括其中可以放置要确定挥发物含量的样品的空腔，用于将微波引入具有基本上不同于红外频率的频率的空腔的第一源，用于将辐射热引入腔中的第二源在与第一源引入的频率不同的频率处，用于测量样品在腔中和平衡时的重量的分析天平，能够测量和定位以测量样品的温度的温度传感器空腔和平衡部，以及与温度传感器和第一和第二源中的每一个通信的处理器，用于响应于由温度传感器测量的温度而将微波和辐射能的频率引入空腔中控制样品温度，直到来自第一个来源的微波和来自th的辐射热第二来源充分干燥样品以使处理器基于样品在天平上的重量变化来确定样品的挥发性含量。

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