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    • 2. 发明授权
    • Immunological detection method of pyrethroid compound, haptenic
compound, intermediate for the haptenic compound, immunogen and antibody
    • 拟除虫菊酯化合物,半抗原化合物,半抗原化合物的中间体,免疫原和抗体的免疫检测方法
    • US5608104A
    • 1997-03-04
    • US310772
    • 1994-09-27
    • Hideo OhkawaKoji KitajimaKenji KodakaYasunori FumotoTakako Kumeta
    • Hideo OhkawaKoji KitajimaKenji KodakaYasunori FumotoTakako Kumeta
    • C07C43/295C07C43/315C07K16/44G01N33/53C07C65/00
    • C07C43/295C07C43/315C07K16/44G01N33/5308
    • Described is a detection method of a pyrethroid compound which comprises providing a first antibody against the pyrethroid compound, said first antibody having been obtained by immunizing a responder with an immunogen which is a first conjugate between a first carrier and a first haptenic compound represented by the following formula (1) or (2): ##STR1## mixing the first antibody with the sample solution, causing any unreacted portion of the first antibody to be bound on a second conjugate as a coated antigen, said second conjugate being formed of a second carrier and a second haptenic compound and having been coated on a surface of a solid phase carrier, whereby an antigen-antibody conjugate is obtained; andreacting the antigen-antibody conjugate with a second antibody against the first antibody, said second antibody having a labelled enzyme linked therewith.This invention has made it possible to conduct easy, economical and quick analysis of a pyrethroid compound contained in our surroundings such as water or soil, plants or foods.
    • 描述了拟除虫菊酯化合物的检测方法,其包括提供针对拟除虫菊酯化合物的第一抗体,所述第一抗体是通过用作为第一载体和第一半抗体化合物所代表的第一半抗原化合物之间的第一偶联物的免疫原免疫应答者获得的 用式(1)或(2)表示:与样品溶液同源,使第一抗体的任何未反应部分作为包被抗原结合在第二结合物上,所述第二结合物由第二载体和第二半抗原化合物 并涂布在固相载体的表面上,得到抗原 - 抗体缀合物; 并使抗原 - 抗体缀合物与针对第一抗体的第二抗体反应,所述第二抗体具有与之连接的标记酶。 本发明使得可以对包含在我们的周围环境中的拟除虫菊酯化合物例如水或土壤,植物或食物进行简单,经济和快速的分析。