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1. 发明申请

US20110229893A1 METHOD OF MEASURING CYTOKERATIN 19 mRNA 审中-公开
标题翻译：测量细胞因子19 mRNA的方法
公开(公告)号：US20110229893A1
公开(公告)日：2011-09-22
申请号：US13131508
申请日：2009-11-30
申请人： Daisuke Omoto , Juichi Saito , Satoru Oonaka , Toshinori Hayashi
发明人： Daisuke Omoto , Juichi Saito , Satoru Oonaka , Toshinori Hayashi
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6853 , C12Q1/6865 , C12Q2525/143 , C12Q2545/114 , C12Q2561/113 , C12Q2531/143 , C12Q2521/107
摘要： Disclosed is a method of amplifying and detecting cytokeratin 19 mRNA in RNA amplification process, comprising: a step for forming a double-stranded DNA containing a promoter sequence with a reverse transcriptase by use of a combination of oligonucleotides consisting of a first primer having a sequence homologous to a portion of cytokeratin 19 mRNA and a second primer having a complementary sequence, wherein the promoter sequence is added to the 5′-end of either the first primer or the second primer, forming an RNA transcription product by use of an RNA polymerase with using the double-stranded DNA as template, and forming the double-stranded DNA by use of a reverse transcriptase by continuing to use the RNA transcription product as a template of DNA synthesis, by measuring an amount of amplified RNA produced over time with an oligonucleotide probe designed so that signal properties change with the formation of a complementary double strand with the amplified RNA.
摘要翻译：公开了在RNA扩增方法中扩增和检测细胞角蛋白19mRNA的方法，其包括：通过使用由具有序列的第一引物组成的寡核苷酸组合形成含逆转录酶启动子序列的双链DNA的步骤与一部分细胞角蛋白19mRNA和具有互补序列的第二引物同源，其中将启动子序列加入第一引物或第二引物的5'端，通过使用RNA聚合酶形成RNA转录产物使用双链DNA作为模板，通过继续使用RNA转录产物作为DNA合成的模板，通过使用逆转录酶形成双链DNA，通过测量随时间推移产生的扩增的RNA的量，寡核苷酸探针被设计成使得信号特性随着扩增的RNA形成互补双链而改变。

2. 发明申请

US20100304377A1 METHOD OF DETECTING NOROVIRUS RNA 审中-公开
标题翻译：检测NOROVIRUS RNA的方法
公开(公告)号：US20100304377A1
公开(公告)日：2010-12-02
申请号：US12666053
申请日：2008-06-12
申请人： Noriyoshi Masuda , Kurando Une , Juichi Saito , Toshinori Hayashi
发明人： Noriyoshi Masuda , Kurando Une , Juichi Saito , Toshinori Hayashi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/701 , C12Q2563/173 , C12Q2531/143 , C12Q2521/107
摘要： The amount of an RNA transcription product amplified in an RNA amplification process is measured using a nucleic acid probe labeled with an intercalating fluorescent dye. The RNA amplification process comprises the steps of using at least two sets of primer pairs comprising a first primer and a second primer (in which one of these primers carries a promoter sequence added to the 5′ end thereof), both of which have high hybridization efficiency to a nucleic acid sequence that is homologous to or complementary to each norovirus genotype RNA; forming a double-stranded DNA containing the promoter sequence with a reverse transcriptase; forming an RNA transcription product with an RNA polymerase by using the double-stranded DNA as a template; and forming the double-stranded DNA by successively using the RNA transcription product as a template in the DNA synthesis with the reverse transcriptase.
摘要翻译：使用用嵌入荧光染料标记的核酸探针测量RNA扩增过程中扩增的RNA转录产物的量。 RNA扩增方法包括使用至少两组包含第一引物和第二引物（其中这些引物中的一个携带其5'末端添加的启动子序列）的引物对，其两者都具有高杂交与每种诺如病毒基因型RNA同源或互补的核酸序列的效率; 用逆转录酶形成含有启动子序列的双链DNA; 通过使用双链DNA作为模板与RNA聚合酶形成RNA转录产物; 并通过在逆转录酶的DNA合成中连续使用RNA转录产物作为模板形成双链DNA。

3. 发明申请

US20100055676A1 METHOD OF ASSAYING ALPHA 1, 4-N-ACETYLGLUCOSAMINE TRANSFERASE (ALPHA 4GNT) MRNA 审中-公开
标题翻译：测定ALPHA 1,4-N-乙酰胆碱转移酶（ALPHA 4GNT）MRNA的方法
公开(公告)号：US20100055676A1
公开(公告)日：2010-03-04
申请号：US11573491
申请日：2005-08-09
申请人： Juichi Saito , Toshinori Hayashi
发明人： Juichi Saito , Toshinori Hayashi
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6865 , C12Q2545/114
摘要： A method for assaying α1,4-N-acetylgiucosamine transferase (α4GnT) mRNA present in a sample, the method comprising a step of using a first primer homologous to at least a portion downstream from the 5′-end of a specified nucleotide sequence of the RNA and a second primer complementary to at least a portion upstream from the 3′-end of the specified nucleotide sequence to produce double-stranded DNA containing the promoter sequence and the specified nucleotide sequence downstream from the promoter sequence, wherein at least one of the first and second primers has a promoter sequence at the 5′-end, a step of using the double-stranded DNA as template to produce an RNA transcript, a step of using the RNA transcript in turn as template for DNA synthesis to produce the double-stranded DNA, a step of nucleic acid amplification in which the aforementioned steps are repeated under conditions that simultaneously promote each of the steps, and a step of assaying the amount of the RNA transcript.
摘要翻译：一种用于测定样品中存在的α1,4-N-乙酰氨基葡糖转移酶（α4GnT）mRNA的方法，所述方法包括使用与特定核苷酸序列的5'末端下游至少部分同源的第一引物的步骤所述RNA和与所述特定核苷酸序列的3'末端上游至少部分互补的第二引物以产生含有所述启动子序列和所述启动子序列下游的特定核苷酸序列的双链DNA，其中至少一个第一和第二引物在5'末端具有启动子序列，使用双链DNA作为模板产生RNA转录物的步骤，依次使用RNA转录物作为DNA合成的模板，生成双链DNA，其中在同时促进每个步骤的条件下重复上述步骤的核酸扩增步骤和测定RNA转移量的步骤 ript。

4. 发明申请

US20080108059A1 Method Of Measuring Heterogeneous Nuclear Ribonucleoprotein B1 (Hnrnp B1) Mrna 审中-公开
标题翻译：测定异质核核糖核蛋白B1（Hnrnp B1）Mrna的方法
公开(公告)号：US20080108059A1
公开(公告)日：2008-05-08
申请号：US11572868
申请日：2005-07-28
申请人： Juichi Saito , Toshinori Hayashi
发明人： Juichi Saito , Toshinori Hayashi
IPC分类号： C12Q1/68
CPC分类号： G01N21/6428 , C12Q1/6865 , C12Q2563/107
摘要： A method for assaying heterogeneous nuclear ribonucleoprotein B1 (hnRNP B1) mRNA present in a sample, the method comprising a step of using a first primer homologous to at least a portion downstream from the 5′-end of a specified nucleotlde sequence of the RNA and a second primer complementary to at least a portion upstream from the 3′-end of the specified nucleotide sequence to produce double-stranded DNA containing the promoter sequence and the specified nucleotide sequence downstream from the promoter sequence, wherein at least one of the first and second primers has a promoter sequence at the 5′-end, a step of using the double-stranded DNA as template to produce an RNA transcript, a step of using the RNA transcript in turn as template for DNA synthesis to produce the double-stranded DNA, a step of nucleic acid amplification in which the aforementioned steps are repeated under conditions that simultaneously promote each of the steps, and a step of assaying the amount of the RNA transcript.
摘要翻译：一种用于测定样品中存在的异源核核糖核蛋白B1（hnRNP B1）mRNA的方法，所述方法包括使用与所述RNA的特定核苷酸序列的5'末端下游至少部分同源的第一引物的步骤，以及与指定核苷酸序列的3'末端上游的至少一部分互补的第二引物，以产生含有启动子序列和启动子序列下游的特定核苷酸序列的双链DNA，其中第一和第第二引物在5'末端具有启动子序列，使用双链DNA作为模板产生RNA转录物的步骤，依次使用RNA转录物作为DNA合成的模板以产生双链的步骤 DNA，在同时促进每个步骤的条件下重复上述步骤的核酸扩增步骤和测定RNA转移量的步骤脚本。

5. 发明授权

US07339041B2 Oligonucleotides and method for characterizing and detecting Genogroup II type small round structured virus 失效
标题翻译：用于表征和检测Genogroup II型小圆结构病毒的寡核苷酸和方法
公开(公告)号：US07339041B2
公开(公告)日：2008-03-04
申请号：US10441126
申请日：2003-05-20
申请人： Noriyoshi Masuda , Takahiko Ishiguro , Juichi Saito , Toshiki Taya , Kiyoshi Yasukawa
发明人： Noriyoshi Masuda , Takahiko Ishiguro , Juichi Saito , Toshiki Taya , Kiyoshi Yasukawa
IPC分类号： C07H21/04
CPC分类号： C12Q1/701
摘要： Nucleic acid sequences, oligonucleotides and a method for detection of SRSV, in particular, a virus which belongs to Genotype II (GII), in clinical examinations, public health examinations, food evaluations and food poisoning examinations are provided.
摘要翻译：提供了核酸序列，寡核苷酸和用于检测SRSV的方法，特别是属于基因型II（GII）的病毒，用于临床检查，公众健康检查，食物评估和食物中毒检查。

6. 发明申请

US20070141595A1 METHOD FOR ASSAYING REG IV mRNA 审中-公开
标题翻译：用于测定REG IV mRNA的方法
公开(公告)号：US20070141595A1
公开(公告)日：2007-06-21
申请号：US11548786
申请日：2006-10-12
申请人： Kurando Une , Juichi Saito , Toshinori Hayashi
发明人： Kurando Une , Juichi Saito , Toshinori Hayashi
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6813
摘要： In an RNA amplification process comprising steps of using a first primer and a second primer, at least one of which has a promoter sequence at the 5′ end, and reverse transcriptase, to produce double-stranded DNA containing the promoter sequence, using the double-stranded DNA as template to produce an RNA transcript with RNA polymerase, and using the RNA transcript in turn as template for DNA synthesis with the reverse transcriptase to produce the double-stranded DNA, the amount of amplified RNA product is measured with an intercalating fluorescent dye-labeled nucleic acid probe.
摘要翻译：在RNA扩增方法中，包括使用第一引物和第二引物，其中至少一个具有5'末端的启动子序列和逆转录酶，以产生含有启动子序列的双链DNA，使用双标记的DNA作为模板，用RNA聚合酶产生RNA转录物，并且使用RNA转录物依次作为用逆转录酶进行DNA合成的模板以产生双链DNA，扩增的RNA产物的量用嵌入荧光染料标记的核酸探针。

7. 发明授权

US06630300B2 Oligonucleotides and method for characterizing and detecting Genogroup II type small round structured virus 失效
公开(公告)号：US06630300B2
公开(公告)日：2003-10-07
申请号：US09989002
申请日：2001-11-21
申请人： Noriyoshi Masuda , Takahiko Ishiguro , Juichi Saito , Toshiki Taya , Kiyoshi Yasukawa
发明人： Noriyoshi Masuda , Takahiko Ishiguro , Juichi Saito , Toshiki Taya , Kiyoshi Yasukawa
IPC分类号： C12Q170
CPC分类号： C12Q1/701
摘要： Nucleic acid sequences, oligonucleotides and a method for detection of SRSV, in particular, a virus which belongs to Genotype II (GII), in clinical examinations, public health examinations, food evaluations and food poisoning examinations are provided.

8. 发明申请

US20110189662A1 METHOD FOR MEASURING SURVIVIN mRNA 审中-公开
标题翻译：测量SURVIVIN mRNA的方法
公开(公告)号：US20110189662A1
公开(公告)日：2011-08-04
申请号：US13122308
申请日：2009-10-06
申请人： Daisuke Omoto , Juichi Saito , Satoru Oonaka , Toshinori Hayashi
发明人： Daisuke Omoto , Juichi Saito , Satoru Oonaka , Toshinori Hayashi
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6886 , C12Q1/6851 , C12Q1/6865 , C12Q2600/158 , C12Q2525/143 , C12Q2563/173
摘要： Disclosed is a method of amplifying and detecting mRNA of survivin gene in an RNA amplification process comprising: a step for forming a double-stranded DNA containing a promoter sequence by use of a combination of oligonucleotides consisting of a first primer having a sequence homologous with a portion of survivin mRNA and a second primer having a complementary sequence, wherein the promoter sequence is added to the 5′-end of either the first primer or the second primer with a reverse transcriptase, forming an RNA transcription product by use of an RNA polymerase with using the double-stranded DNA as template, and forming the double-stranded DNA by use of a reverse transcriptase by continuing to use the RNA transcription product as a template of DNA synthesis, measuring an amount of amplified RNA produced over time with an oligonucleotide probe designed so that signal properties change with the formation of a complementary double strand with the amplified RNA.
摘要翻译：公开了在RNA扩增方法中扩增和检测存活蛋白基因的mRNA的方法，其包括：通过使用由具有与第一引物序列同源的第一引物组成的寡核苷酸组合形成含有启动子序列的双链DNA的步骤存在蛋白mRNA的部分和具有互补序列的第二引物，其中通过逆转录酶将启动子序列添加到第一引物或第二引物的5'-末端，通过使用RNA聚合酶形成RNA转录产物使用双链DNA作为模板，并通过继续使用RNA转录产物作为DNA合成的模板，通过使用逆转录酶形成双链DNA，用寡核苷酸测量随时间产生的扩增的RNA的量探针的设计使得信号特性随扩增的RNA形成互补双链而发生变化。

9. 发明申请

US20100297637A1 PRIMER FOR AMPLIFICATION OF RRNA OR BACTERIUM BELONGING TO THE GENUS LEGIONELLA, DETECTION METHOD, AND DETECTION KIT 审中-公开
标题翻译：用于扩增与基因落叶松相关的RRNA或细菌的检测方法和检测试剂盒
公开(公告)号：US20100297637A1
公开(公告)日：2010-11-25
申请号：US12681557
申请日：2008-10-01
申请人： Kurando Une , Juichi Saito , Toshinori Hayashi , Fumihide Takizawa , Yoichi Hashimoto
发明人： Kurando Une , Juichi Saito , Toshinori Hayashi , Fumihide Takizawa , Yoichi Hashimoto
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689 , Y02A50/451
摘要： To detect a bacterium belonging to the genus Legionella rapidly, with high sensitivity, and specifically. Disclosed are: a primer set for amplifying ribosomal RNA of a bacterium belonging to the genus Legionella specifically and highly efficiently; and a method for detecting ribosomal RNA of a bacterium belonging to the genus Legionella by using the primer set.
摘要翻译：快速检测属于军团菌属的细菌，具有高灵敏度，特异性。公开了一种用于特异性和高效地扩增属于军团菌属细菌的核糖体RNA的引物组; 以及通过使用引物组检测属于军团菌属的细菌的核糖体RNA的方法。

10. 发明申请

US20090191562A1 METHOD FOR ASSAYING REG IV mRNA 审中-公开
标题翻译：用于测定REG IV mRNA的方法
公开(公告)号：US20090191562A1
公开(公告)日：2009-07-30
申请号：US12354314
申请日：2009-01-15
申请人： Kurando UNE , Juichi Saito , Toshinori Hayashi
发明人： Kurando UNE , Juichi Saito , Toshinori Hayashi
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6813
摘要： In an RNA amplification process comprising steps of using a first primer and a second primer, at least one of which has a promoter sequence at the 5′ end, and reverse transcriptase, to produce double-stranded DNA containing the promoter sequence, using the double-stranded DNA as template to produce an RNA transcript with RNA polymerase, and using the RNA transcript in turn as template for DNA synthesis with the reverse transcriptase to produce the double-stranded DNA, the amount of amplified RNA product is measured with an intercalating fluorescent dye-labeled nucleic acid probe.
摘要翻译：在RNA扩增方法中，包括使用第一引物和第二引物，其中至少一个具有5'末端的启动子序列和逆转录酶，以产生含有启动子序列的双链DNA，使用双标记的DNA作为模板，用RNA聚合酶产生RNA转录物，并且使用RNA转录物依次作为用逆转录酶进行DNA合成的模板以产生双链DNA，扩增的RNA产物的量用嵌入荧光染料标记的核酸探针。

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