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1. 发明申请

WO2004033059A2 METHODS AND APPARATUS FOR OPTOPHORETIC DIAGNOSIS OF CELLS AND PARTICLES 审中-公开
标题翻译：细胞和颗粒的OPTOPHORETIC诊断的方法和装置
公开(公告)号：WO2004033059A2
公开(公告)日：2004-04-22
申请号：PCT/US2003/030975
申请日：2003-09-30
申请人： GENOPTIX, INC. , ZHANG, Haichuan , CHUNG, Thomas, D.Y. , HALL, Jeff , SOOHOO, William , KOHRUMEL, Josh , TU, Eugene , WANG, Mark , RAYMOND, Daniel, Edward , MARCHAND, Philippe , DIVER, Jonathan , BUTLER, William, F. , NGUYEN, Phan , CHACHISVILIS, Mirianas , KATZ, Andrew , HAGEN, Norbert , LYKSTAD, Kris , PESTANA, Luis
发明人： ZHANG, Haichuan , CHUNG, Thomas, D.Y. , HALL, Jeff , SOOHOO, William , KOHRUMEL, Josh , TU, Eugene , WANG, Mark , RAYMOND, Daniel, Edward , MARCHAND, Philippe , DIVER, Jonathan , BUTLER, William, F. , NGUYEN, Phan , CHACHISVILIS, Mirianas , KATZ, Andrew , HAGEN, Norbert , LYKSTAD, Kris , PESTANA, Luis
IPC分类号： B01C
CPC分类号： G01N33/56966 , G01N15/147 , G01N15/1484 , G01N2015/1006 , G01N2015/1075 , G01N2015/145
摘要： A device for characterizing a cell or particle includes a channel having an inlet and an outlet, the channel containing a moving fluid therein for carrying the cell or particle from the inlet to the outlet. The device includes a detector for detecting the presence of a cell or particle along portion of the channel, the detector including a first detecting position, a second detecting position, and a third detecting position. The device further includes a light source providing an optical gradient disposed within the channel and between the second and third detecting positions. A control system is coupled to the detector to receive and process detected signals from the detector. During operation, the amount of time that a cell or particle takes to flow through a first distance (i.e., its time-of-flight) is measured. The cell or particle is then flowed past a second, downstream distance in the presence of an optical gradient and its time-of-flight is measured. A comparison of the measured time-of-flights for the first and second distances is used to characterize the cell or particle. The method can be used to characterize and sort cells based on a biological property.
摘要翻译：用于表征细胞或颗粒的装置包括具有入口和出口的通道，所述通道在其中包含运动流体，用于将细胞或颗粒从入口运送到出口。该装置包括用于沿着通道的一部分检测单元或颗粒的存在的检测器，检测器包括第一检测位置，第二检测位置和第三检测位置。该装置还包括提供设置在通道内且在第二和第三检测位置之间的光学梯度的光源。控制系统耦合到检测器以接收和处理来自检测器的检测信号。在操作期间，测量细胞或颗粒流过第一距离（即其飞行时间）所花费的时间量。然后在存在光学梯度的情况下将电池或颗粒流过第二个下游距离，并测量其飞行时间。使用测量的第一和第二距离的飞行时间的比较来表征单元或颗粒。该方法可用于基于生物学特性来表征和分类细胞。

2. 发明申请

WO0062036A9 AMPLIFICATION AND SEPARATION OF NUCLEIC ACID SEQUENCES USING STRAND DISPLACEMENT AMPLIFICATION AND BIOELECTRONIC MICROCHIP TECHNOLOGY 审中-公开
标题翻译：使用带状位移扩增和生物电子微波技术的核酸序列的扩增和分离
公开(公告)号：WO0062036A9
公开(公告)日：2002-02-21
申请号：PCT/US0009711
申请日：2000-04-11
申请人： NANOGEN BECTON DICKINSON PARTN , NERENBERG MICHAEL I , EDMAN CARL F , SPARGO CATHERINE A , WALKER GEORGE T , CARRINO JOHN J , GERRUE LOUIS O , DIVER JONATHAN M
发明人： NERENBERG MICHAEL I , EDMAN CARL F , SPARGO CATHERINE A , WALKER GEORGE T , CARRINO JOHN J , GERRUE LOUIS O , DIVER JONATHAN M
IPC分类号： G01N33/53 , C12Q1/68 , G01N1/28 , G01N21/78 , G01N37/00 , G01N15/06 , C12P19/34
CPC分类号： C12Q1/6844 , C12P19/34 , C12Q1/6825 , C12Q1/6837 , C12Q2537/143 , C12Q2531/119 , C12Q2565/501 , C12Q2565/607
摘要： Described and disclosed are devices, methods, and compositions of matter for the multiplex amplification and analysis of nucleic acid sequences in a sample using novel strand displacement amplification technologies in combination with bioelectronic microchip technology. Specifically, a nucleic acid in a sample is amplified to form amplicons, the amplicons are addressed to specified electronically addressable capture sites of the bioelelectronic microchip, the addressed amplicons are captured and labeled, and then the capture sites are analyzed for the presence of label. Samples may be amplified using strand displacement amplification. The invention is also amenable to other amplification methodologies well known by those skilled in the art. The capture and label steps may be by a method of universal capture with sequence specific reporter, or by a method of sequence specific capture with universal reporter. The label may be detected by fluorescence, chemiluminescence, electrochemiluminescence, or any other technique as are well known by those skilled in the art. This invention further allows for analyzing multiple nucleic acid targets on a single diagnostic platform wherein the nucleic acids may be amplified while either in direct contact with microchip components or in solution above the microchip array.
摘要翻译：描述和公开的是使用新颖的链置换扩增技术结合生物电子微芯片技术在样品中多重扩增和分析核酸序列的物质的装置，方法和组成。具体地说，样品中的核酸被扩增以形成扩增子，扩增子被寻址到生物电子微芯片的指定的可电子寻址的捕获位点，被寻址的扩增子被捕获和标记，然后分析捕获位点的标记物的存在。可以使用链置换扩增来扩增样品。本发明还适用于本领域技术人员熟知的其它扩增方法。捕获和标记步骤可以通过使用序列特异性报告物通用捕获的方法，或通过使用通用报告物的序列特异性捕获的方法。可以通过荧光，化学发光，电化学发光或本领域技术人员公知的任何其它技术来检测标记。本发明还允许在单个诊断平台上分析多个核酸靶标，其中核酸可以在与微芯片部件直接接触或在微芯片阵列上方的溶液中被放大。

3. 发明申请

WO2008048931A1 METHODS AND COMPOSITIONS FOR DIFFERENTIAL EXPANSION OF FETAL CELLS IN MATERNAL BLOOD AND THEIR USE 审中-公开
标题翻译：方法和组合物在宫颈细胞中的差异扩大及其使用
公开(公告)号：WO2008048931A1
公开(公告)日：2008-04-24
申请号：PCT/US2007/081396
申请日：2007-10-15
申请人： CELULA INC. , MCNEELEY, Patricia , MARCHAND, Philippe , DIVER, Jonathan
发明人： MCNEELEY, Patricia , MARCHAND, Philippe , DIVER, Jonathan
IPC分类号： C12N5/06 , C12N5/00
CPC分类号： C12N5/0634 , C12N2500/90 , C12N2500/98 , C12N2501/125 , C12N2501/23
摘要： Disclosed is a method and compositions for the differential expansion of fetal cells over maternal cells. In the method, cells from a sample of maternal blood containing CD34+ cells of both maternal and fetal origin are incubated in the presence of Stem Cell Factor in serum free media. It has been discovered that incubation of fetal cells in the presence of SCF will preferentially expand the fetal cells relative to adult cells. Fetal cells can also be identified, enriched or obtained by differential expansion of the fetal cells during colony formation. It has been discovered that differential expansion of fetal cells can result in colonies of fetal cells that are larger than colonies of adult cells. The fetal CD34+ cells can be expanded without generation of significant clonal genetic artifacts during expansion. Also disclosed is a method and compositions for producing differentiated fetal cells. It has been discovered that differentiated fetal cells have markers that distinguish the fetal cells from adult cells. Also disclosed are fetal cells made or obtained using the disclosed methods. For example, disclosed are expanded and/or differentiated fetal cells. The disclosed fetal cells can be used for any purpose and in any way that fetal cells can be used. The disclosed fetal cells are particularly useful for prenatal analysis of a gestating fetus.
摘要翻译：公开了胎儿细胞在母细胞上差异扩增的方法和组合物。在该方法中，含有母体和胎儿来源的含有CD34 +细胞的母体血液样品的细胞在无血清培养基中的干细胞因子存在下孵育。已经发现，在SCF存在下胎儿细胞的孵育将相对于成体细胞优先扩张胎儿细胞。胎儿细胞也可以在菌落形成期间通过胎儿细胞的差异扩增来鉴定，富集或获得。已经发现，胎儿细胞的差异扩增可导致比成体细胞的集落大的胎儿细胞的集落。胎儿CD34 +细胞可以在扩增期间扩增而不产生显着的克隆遗传伪像。还公开了用于产生分化的胎儿细胞的方法和组合物。已经发现，分化的胎儿细胞具有区分胎儿细胞和成体细胞的标记。还公开了使用所公开的方法制造或获得的胎儿细胞。例如，所公开的是扩增和/或分化的胎儿细胞。所公开的胎儿细胞可以用于任何目的，并且可以以任何方式使用胎儿细胞。所公开的胎儿细胞特别可用于孕胎分娩。

4. 发明申请

WO2003093496A1 METHOD OF USING OPTICAL INTERROGATION TO DETERMINE A BIOLOGICAL PROPERTY OF A CELL OR POPULATION OF CELLS 审中-公开
标题翻译：使用光学干涉来确定细胞或细胞群体的生物学特性的方法
公开(公告)号：WO2003093496A1
公开(公告)日：2003-11-13
申请号：PCT/US2003/013735
申请日：2003-04-30
申请人： GENOPTIX, INC. , SCHNABEL, Catherine, A. , DIVER, Jonathan , KARIV, Ilona , FORSTER, Anita , MERCER, Elinore , HALL, Jeffrey , NOVA, Tina , SOOHOO, William , KOHRUMEL, Josh , NGUYEN, Phan , ZHANG, Haichuan , TU, Eugene , CHUNG, Thomas, D., Y. , LYKSTAD, Kristie, Lynn , WANG, Mark, M. , BUTLER, William, Frank , RAYMOND, Daniel, E.
发明人： SCHNABEL, Catherine, A. , DIVER, Jonathan , KARIV, Ilona , FORSTER, Anita , MERCER, Elinore , HALL, Jeffrey , NOVA, Tina , SOOHOO, William , KOHRUMEL, Josh , NGUYEN, Phan , ZHANG, Haichuan , TU, Eugene , CHUNG, Thomas, D., Y. , LYKSTAD, Kristie, Lynn , WANG, Mark, M. , BUTLER, William, Frank , RAYMOND, Daniel, E.
IPC分类号： C12Q1/00
CPC分类号： G01N33/5091 , G01N33/5008 , G01N33/5011 , G01N33/5014 , G01N33/502 , G01N33/505 , G01N2510/00
摘要： Optophoretic methods are used to determine one or more biological properties or changes in biological properties of one or more cells or cellular components. The methods use optical or photonic forces to select, identify, characterize, and/or sort whole cells or groups of cells. The methods are useful in a number of applications, including, but not limited to, drug screening applications, toxicity applications, protein expression applications, rapid clonal selection applications, biopharmaceutical monitoring and quality control applications, cell enrichment applications, viral detection, bacterial drug sensitivity screening, environmental testing, agricultural testing, food safety testing, personalized medicine applications as well as biohazard detection and analysis.
摘要翻译：荧光学方法用于确定一种或多种细胞或细胞组分的一种或多种生物学性质或生物学特性的变化。该方法使用光学或光子力来选择，识别，表征和/或分类整个细胞或细胞组。该方法在许多应用中是有用的，包括但不限于药物筛选应用，毒性应用，蛋白质表达应用，快速克隆选择应用，生物药物监测和质量控制应用，细胞富集应用，病毒检测，细菌药物敏感性筛选，环境检测，农业检测，食品安全检测，个性化医疗应用以及生物危害检测和分析。

5. 发明申请

WO2004033059A3 METHODS AND APPARATUS FOR OPTOPHORETIC DIAGNOSIS OF CELLS AND PARTICLES 审中-公开
标题翻译：细胞和颗粒的OPTOPHORETIC诊断的方法和装置
公开(公告)号：WO2004033059A3
公开(公告)日：2006-01-26
申请号：PCT/US0330975
申请日：2003-09-30
申请人： GENOPTIX INC , ZHANG HAICHUAN , CHUNG THOMAS D Y , HALL JEFF , SOOHOO WILLIAM , KOHRUMEL JOSH , TU EUGENE , WANG MARK , RAYMOND DANIEL EDWARD , MARCHAND PHILIPPE , DIVER JONATHAN , BUTLER WILLIAM F , NGUYEN PHAN , CHACHISVILIS MIRIANAS , KATZ ANDREW , HAGEN NORBERT , LYKSTAD KRIS , PESTANA LUIS
发明人： ZHANG HAICHUAN , CHUNG THOMAS D Y , HALL JEFF , SOOHOO WILLIAM , KOHRUMEL JOSH , TU EUGENE , WANG MARK , RAYMOND DANIEL EDWARD , MARCHAND PHILIPPE , DIVER JONATHAN , BUTLER WILLIAM F , NGUYEN PHAN , CHACHISVILIS MIRIANAS , KATZ ANDREW , HAGEN NORBERT , LYKSTAD KRIS , PESTANA LUIS
IPC分类号： G01N33/48 , G01N33/569 , G21K1/00 , C12Q1/00 , C12M1/00 , G01F23/00 , G01N33/558
CPC分类号： G01N33/56966 , G01N15/147 , G01N15/1484 , G01N2015/1006 , G01N2015/1075 , G01N2015/145
摘要： A device for characterizing a cell or particle includes a channel having an inlet and an outlet, the channel containing a moving fluid therein for carrying the cell or particle from the inlet to the outlet. The device includes detectors for monitorng the position of the cell or particle along the channel. The device further comprises a light source to provide an optical gradient and a control system to receive and process signals from the detector.The device can be used to characterize and sort cells based on a biological property.
摘要翻译：用于表征细胞或颗粒的装置包括具有入口和出口的通道，所述通道在其中包含运动流体，用于将细胞或颗粒从入口运送到出口。该装置包括用于监测细胞或颗粒沿着通道的位置的检测器。该装置还包括提供光学梯度的光源和用于接收和处理来自检测器的信号的控制系统。该装置可用于基于生物特性来表征和分类细胞。

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