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    • 1. 发明申请
    • ALGAL LIPID PRODUCTION
    • ALGAL脂生产
    • US20120088279A1
    • 2012-04-12
    • US13319745
    • 2010-05-11
    • Andrew Keith SwansonBradley Lynn PostlerRichard T. Sayre
    • Andrew Keith SwansonBradley Lynn PostlerRichard T. Sayre
    • C12P7/64C12M1/00C12N1/12
    • C12P7/6463
    • Methods for increasing the levels of lipids in oleaginous algae are described. Lipid levels in algae can be increased by stress, such as nutrient stress, after which the lipid can be harvested from the algae using a non-destructive extraction process. The stress may be provided in a periodic or “pulsed” fashion. Lipid levels in oleaginous algae can also be increased using simulated stress by treating the algae with a chemical inhibitor or by using recombinant technology to insert a sequence expressing a protein such as a nitrate reductase inhibitor that is expressed when a stressed state is desired. A method for maintaining the temperature and water levels of algae ponds using buoyant spheres is also described.
    • 描述了提高含油藻类脂质含量的方法。 藻类中的脂质水平可以通过胁迫(如营养胁迫)增加,此后可以使用非破坏性提取过程从藻类中收获脂质。 应力可以以周期性或“脉冲”的方式提供。 通过用化学抑制剂处理藻类或通过使用重组技术插入表达当需要应激状态时表达的蛋白质例如硝酸还原酶抑制剂的序列,也可以使用模拟应力来增加油质藻类中的脂质水平。 还描述了使用浮力球保持藻类池塘的温度和水位的方法。