专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

2. 发明授权

US5529920A Human liver epithelial cell line and culture media therefor 失效
标题翻译：人肝上皮细胞系及其培养基
公开(公告)号：US5529920A
公开(公告)日：1996-06-25
申请号：US879165
申请日：1992-05-01
申请人： Katharine H. Cole , John F. Lechner , Roger Reddel , Curtis C. Harris , Andrea M. Pfeifer
发明人： Katharine H. Cole , John F. Lechner , Roger Reddel , Curtis C. Harris , Andrea M. Pfeifer
IPC分类号： C12N5/071 , C12Q1/04 , G01N33/50 , C12N5/06 , A01N63/00 , C12N5/10 , C12Q1/02
CPC分类号： C12N5/067 , C12Q1/045 , G01N33/5017 , C12N2500/14 , C12N2500/20 , C12N2500/22 , C12N2500/25 , C12N2500/32 , C12N2500/84 , C12N2500/90 , C12N2501/01 , C12N2501/11 , C12N2501/385 , C12N2501/39 , C12N2501/395 , C12N2510/04 , G01N2333/914
摘要： The present invention relates to long term multiplication and permanent establishment of a cell line of human liver epithelial cells(hepatocytes). The human liver epithelial cell line is capable of mitotically proliferating and continuously growing in vitro under suitable environmental conditions in suitable culture media. A method of producing an immortalized human liver epithelial cell line is also disclosed. The invention also relates to serum-free cell medium developed to support long term multiplication and permanent establishment of a cell line of human liver epithelial cells. The medium may contain an effective cell growth promoting amount of calcium ions; an effective cell growth promoting amount of glucose; an effective amount of insulin to aid cells in glucose uptake; an effective cell growth promoting amount of hydrocortisone; an effective amount of epidermal growth factor to bind epidermal growth factor receptors on cells; an effective amount of transferrin to increase DNA synthesis in cells; an effective amount of cholera toxin to increase DNA synthesis in cells; an effective amount of triiodothyronine to increase DNA synthesis in cells; and an effective growth promoting amount of mammalian hormones and mitogenic factors, including lipoprotein, cholesterol, phospholipids and fatty acids.
摘要翻译：本发明涉及长期繁殖和永久建立人肝上皮细胞（肝细胞）的细胞系。人类肝上皮细胞系能够在合适的环境条件下在合适的培养基中体外增殖并连续生长。还公开了永生化人肝上皮细胞系的制备方法。本发明还涉及开发用于支持长期繁殖和永久建立人肝上皮细胞细胞系的无血清细胞培养基。介质可能含有有效的细胞生长促进量的钙离子; 有效的细胞生长促进量的葡萄糖; 有效量的胰岛素以帮助细胞摄取葡萄糖; 有效的细胞生长促进量的氢化可的松; 有效量的表皮生长因子结合细胞上的表皮生长因子受体; 有效量的转铁蛋白增加细胞中的DNA合成; 有效量的霍乱毒素以增加细胞中的DNA合成; 有效量的三碘甲状腺原氨酸增加细胞中的DNA合成; 以及哺乳动物激素和促有丝分裂因子的有效生长促进量，包括脂蛋白，胆固醇，磷脂和脂肪酸。

IPRDB

热门服务

关于我们

友情链接

联系方式