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1. 发明申请

WO0109352A3 USE OF ASPERGILLUS SOJAE AS HOST FOR RECOMBINANT PROTEIN PRODUCTION 审中-公开
标题翻译：用作重组蛋白生产的“ASPERGILLUS”SOJAE作为主机的使用
公开(公告)号：WO0109352A3
公开(公告)日：2001-11-08
申请号：PCT/NL0000544
申请日：2000-07-28
申请人： TNO , HOFFMANN LA ROCHE , HEERIKHUISEN MARGREET , DEN HONDEL CORNELIS VAN , PUNT PETER , BIEZEN NICK VAN , ALBERS ALWIN , VOGEL KURT
发明人： HEERIKHUISEN MARGREET , VAN DEN HONDEL CORNELIS , PUNT PETER , VAN BIEZEN NICK , ALBERS ALWIN , VOGEL KURT
IPC分类号： C12N15/09 , C12N1/14 , C12N1/15 , C12N9/16 , C12N9/58 , C12N15/10 , C12N15/67 , C12N15/80 , C12R1/66
CPC分类号： C12N15/80 , C12N9/58
摘要： A recombinant Aspergillus sojae comprising an introduced acetamidase S ( amdS ) gene as selectable marker is disclosed. An Aspergillus sojae exhibiting growth with medium comprising uracil and fluoro-orotic acid, said Aspergillus sojae further not exhibiting growth on medium comprising uridine and fluoro-orotic acid i.e. said Aspergillus sojae exhibiting uracil auxotrophy, said Aspergillus sojae being unable to utilize uridine, said Aspergillus sojae being pyrG negative, said Aspergillus sojae exhibiting resistance to fluoro-orotic acid, said uracil auxotrophy and said fluoro-orotic acid resistance being relievable upon complementation with an active introduced pyrG gene, is described. The Aspergillus sojae further comprises a nucleic acid sequence encoding a phytase or a protein having phytase activity or any other heterologous protein or polypeptide and can be used for the biotechnological production of said phytase or said other heterologous proteins or polypeptides. Additional mutants exhibiting amended morphology are also disclosed. Methods of producing such expression hosts are described.
摘要翻译：公开了包含引入的乙酰胺酶S（amdS）基因作为选择标记的重组曲霉大肠杆菌。表达与包含尿嘧啶和氟代乳清酸的培养基一起生长的曲霉菌，所述的曲霉大肠杆菌在包含尿苷和氟 - 乳清酸的培养基上进一步不显示生长，即所述的< 表现出尿嘧啶营养缺陷型的曲霉大肠杆菌（Aspergillus sojae），所说的曲霉菌（Aspergillus sojae）不能利用尿苷，所说的曲霉菌（Aspergillus sojae）是pyrG阴性的，所说的Aspergillus sojae 表现出对氟乳清酸的抗性，所述尿嘧啶营养缺陷型和所述氟 - 乳清酸抗性在与活性引入的pyrG基因互补时可缓解。曲霉菌还包含编码植酸酶或具有植酸酶活性的蛋白质或任何其它异源蛋白质或多肽的核酸序列，并可用于生物技术生产所述植酸酶或所述其它异源蛋白质或多肽。还公开了表现出修饰形态的另外的突变体。描述了产生这种表达宿主的方法。

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