基本信息:
- 专利标题： ULTRASONIC GENETICALLY ENCODED CALCIUM INDICATORS
- 申请号：PCT/US2022/077555 申请日：2022-10-04
- 公开(公告)号：WO2023060095A1 公开(公告)日：2023-04-13
- 发明人： JIN, Zhiyang , SHAPIRO, Mikhail G. , LAKSHMANAN, Anupama , TRAN, Teresa
- 申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY
- 申请人地址： 1200 E. California Blvd., M/C 6-32
- 专利权人： CALIFORNIA INSTITUTE OF TECHNOLOGY
- 当前专利权人： CALIFORNIA INSTITUTE OF TECHNOLOGY
- 当前专利权人地址： 1200 E. California Blvd., M/C 6-32
- 代理机构： LIU, Jing
- 优先权： US63/252,499 2021-10-05
- 主分类号： C07K14/195
- IPC分类号： C07K14/195 ; A61K49/22 ; A61B8/08 ; C07K14/005 ; C07K14/215 ; C07K2319/00 ; C12N15/625 ; C12N15/85 ; C12N2503/00 ; C12N2800/107 ; C12N5/0686 ; C12Q1/6897
摘要：
Disclosed herein include methods, compositions, and kits suitable for use in calcium imaging. There are provided, in some embodiments, Ca2+-sensing GvpC proteins. Disclosed herein include Ca2+-sensing gas vesicles (GVs) comprising Ca2+-sensing GvpC proteins. In some embodiments, the Ca2+-sensing GvpC protein is capable of undergoing a first allosteric conformational change upon the Ca2+-binding domain binding Ca2+ that causes the Ca2+-sensing GV to change from a GV stiff state to a GV soft state. One or more of the mechanical, acoustic, surface, and magnetic properties of a Ca2+-sensing GV can differ between the GV soft state and the GV stiff state.
IPC结构图谱:
| C | 化学；冶金 |
| --C07 | 有机化学 |
| ----C07K | 肽 |
| ------C07K14/00 | 具有多于20个氨基酸的肽；促胃液素；生长激素释放抑制因子；促黑激素；其衍生物 |
| --------C07K14/195 | .来自细菌 |