基本信息:
- 专利标题： METHOD FOR REDUCING ENDOTOXIN LEVELS IN NUCLEIC ACID PURIFICATION
- 申请号：PCT/EP2022/071786 申请日：2022-08-03
- 公开(公告)号：WO2023012206A1 公开(公告)日：2023-02-09
- 发明人： HEINEN-KREUZIG, Anja , KIESEWETTER, Andre , STEIN, Andreas , GUPTA, Akshat
- 申请人： MERCK PATENT GMBH
- 申请人地址： Frankfurter Strasse 250
- 专利权人： MERCK PATENT GMBH
- 当前专利权人： MERCK PATENT GMBH
- 当前专利权人地址： Frankfurter Strasse 250
- 优先权： US63/229,666 2021-08-05
- 主分类号： C12N15/10
- IPC分类号： C12N15/10
摘要：
The present invention relates to a method for reducing endotoxin levels or removing endotoxins from nucleic acids. For this a non-ionic detergent selected from the group of alkylglycosides and secondary alcohol alkoxylates or mixtures thereof is added prior or during anion exchange chromatographic purification of the nucleic acids using a membrane or monolith-based sorbent.