基本信息:
- 专利标题： METHODS AND CONSTRUCTS FOR PRODUCTION OF LENTIVIRAL VECTOR
- 申请号：PCT/US2020/047650 申请日：2020-08-24
- 公开(公告)号：WO2021041322A1 公开(公告)日：2021-03-04
- 发明人： SHIN, Young , SETH, Anandita , GU, Bingnan
- 申请人： LONZA WALKERSVILLE, INC.
- 申请人地址： 8830 Biggs Ford Road
- 专利权人： LONZA WALKERSVILLE, INC.
- 当前专利权人： LONZA WALKERSVILLE, INC.
- 当前专利权人地址： 8830 Biggs Ford Road
- 代理机构： MILLS, Jeffrey K.
- 优先权： US62/890,904 2019-08-23
- 主分类号： C12N15/86
- IPC分类号： C12N15/86 ; C12N15/85 ; C12N5/10 ; C12N7/00 ; C12N15/62
摘要：
The present disclosure relates to methods for producing lentiviral vector-producing cells. Specifically the methods utilize two plasmids, rather than four, to provide the required packaging elements and transfer vector to a cell, allowing for the production of a large number of lentiviral producer cells, including suspension-based cells, and the production of high amounts of lentivirus. These methods allow for the production of cells that can be later induced to produce lentivirus, and can be tailored to include a specific gene of interest.