The present invention is directed to an affinity chromatography device that has a normal flow and which separates a targeted protein from aqueous mixtures. The chromatography device includes a housing containing therein a spiral wound membrane assembly that includes at least one inner intermediate material that forms an outer flow channel, at least one polymer membrane that contains therein inorganic particles, and at least one outer intermediate material that forms an inner flow channel sequentially positioned around a central core having a solid outer wall. An aqueous mixture is passed through the outer flow channel, through the polymer membrane where the targeted protein is removed, and then through an inner flow channel. The affinity chromatography device further includes an inlet flow distributor containing an inlet and an outlet flow distributer containing an outlet. Additionally, the chromatography device has a dimensionless resistance parameter that is less than 0. 08.