基本信息:
- 专利标题: COLORIMETRIC SENSING POLYMERASE CHAIN REACTION (PCR)
- 申请号:PCT/US2019/050982 申请日:2019-09-13
- 公开(公告)号:WO2020060855A1 公开(公告)日:2020-03-26
- 发明人: ZHU, Guozhi , QIAO, Miao
- 申请人: GM BIOSCIENCES, INC
- 申请人地址: 5350 Partners Court, Suite C Frederick, Maryland 21703 US
- 专利权人: GM BIOSCIENCES, INC
- 当前专利权人: GM BIOSCIENCES, INC
- 当前专利权人地址: 5350 Partners Court, Suite C Frederick, Maryland 21703 US
- 优先权: US62/733,853 20180920
- 主分类号: C09B29/02
- IPC分类号: C09B29/02 ; C09B29/12 ; C09B29/16 ; C09B29/36 ; C12N15/10 ; C12P19/34
摘要:
Methods are provided here for an easy and reliable non-fluorescent approach to detecting and monitoring PCR amplification. This includes monitoring PCR amplification by using magnesium-sensitive colorimetric dye. The dye is visually detectable color dye. Compositions are described here that comprise a magnesium-sensitive colorimetric dye, a buffer, dNTPs, magnesium ion and DNA polymerase.
IPC结构图谱:
C | 化学;冶金 |
--C09 | 染料;涂料;抛光剂;天然树脂;黏合剂;其他类目不包含的组合物;其他类目不包含的材料的应用 |
----C09B | 有机染料或用于制造染料的有关化合物;媒染剂;色淀 |
------C09B29/00 | 由重氮化及偶合制备的单偶氮染料 |
--------C09B29/01 | .以重氮组分为特征的 |
----------C09B29/02 | ..由重氮化的邻-氨基-羟基化合物 |