发明申请
WO2019173637A1 NANOCELLULOSE-CONTAINING BIOINKS FOR 3D BIOPRINTING, METHODS OF MAKING AND USING THE SAME, AND 3D BIOSTRUCTURES OBTAINED THEREFROM
审中-公开
基本信息:
- 专利标题： NANOCELLULOSE-CONTAINING BIOINKS FOR 3D BIOPRINTING, METHODS OF MAKING AND USING THE SAME, AND 3D BIOSTRUCTURES OBTAINED THEREFROM
- 申请号：PCT/US2019/021225 申请日：2019-03-07
- 公开(公告)号：WO2019173637A1 公开(公告)日：2019-09-12
- 发明人： NELSON, Kimberly , WHITAKER, Iain , JESSOP, Zita , AL-SABAH, Ayesha
- 申请人： GRANBIO INTELLECTUAL PROPERTY HOLDINGS, LLC , SWANSEA UNIVERSITY
- 申请人地址： Attn: Ryan O'Connor c/o PO Box 580 Minnetrista, Minnesota 55364 US
- 专利权人： GRANBIO INTELLECTUAL PROPERTY HOLDINGS, LLC,SWANSEA UNIVERSITY
- 当前专利权人： GRANBIO INTELLECTUAL PROPERTY HOLDINGS, LLC,SWANSEA UNIVERSITY
- 当前专利权人地址： Attn: Ryan O'Connor c/o PO Box 580 Minnetrista, Minnesota 55364 US
- 代理机构： O'CONNOR, Ryan
- 优先权： US62/639,538 20180307
- 主分类号： A61L27/26
- IPC分类号： A61L27/26 ; A61L27/38 ; A61L27/48 ; A61L27/56 ; B33Y80/00 ; B33Y70/00
摘要：
Some variations of the invention provide a bioink composition for 3D bioprinting, comprising: nanocellulose in the form of nanocellulose crystals, nanocellulose fibrils, or preferably a combination thereof; alginate that is ionically crosslinkable in the presence of an ionic crosslinking agent; and water. The nanocellulose-alginate bioinks have favorable rheological, swelling, and biocompatibility properties for extrusion-based bioprinting. It is experimentally demonstrated that nanocellulose-alginate bioinks with human nasoseptal chondrocytes enable cartilage bioprinting at high resolution. The disclosed nanocellulose has been proven to be compatible with cell survival and proliferation; to possess nanoscale and microscale architectures mimicking the native extracellular environment, encouraging differentiation and tissue formation; and to have ideal rheological properties to allow extrusion 3D bioprinting. Bioink with a unique blend of crystal and fibril nanocellulose produces a very stable construct volume in culture.
IPC结构图谱:
| A | 人类生活必需 |
| --A61 | 医学或兽医学；卫生学 |
| ----A61L | 材料或物体消毒的一般方法或装置；空气的灭菌、消毒或除臭；绷带、敷料、吸收垫或外科用品的化学方面；绷带、敷料、吸收垫或外科用品的材料 |
| ------A61L27/00 | 假体材料或假体被覆材料 |
| --------A61L27/02 | .无机材料 |
| ----------A61L27/26 | ..大分子物质的混合物 |