基本信息:
- 专利标题： SEQUENCING LIBRARY PREPARATION IN SMALL WELL FORMAT
- 申请号：PCT/US2018/015648 申请日：2018-01-29
- 公开(公告)号：WO2018144363A1 公开(公告)日：2018-08-09
- 发明人： LAI, Henry H. , CHU, Clement S.
- 申请人： COUNSYL, INC.
- 申请人地址： 180 Kimball Way South San Francisco, California 94080 US
- 专利权人： COUNSYL, INC.
- 当前专利权人： COUNSYL, INC.
- 当前专利权人地址： 180 Kimball Way South San Francisco, California 94080 US
- 代理机构： BOYD, Victoria, L. et al.
- 优先权： US62/452,898 20170131
- 主分类号： C12N15/10
- IPC分类号： C12N15/10 ; C12Q1/68
摘要：
Disclosed are high concentration reagents for use in preparing DNA samples in low volume reactions. Such reagents include, for example, DNA end repair buffers for use in low volume DNA blunting and phosphorylating reactions, DNA adenylating buffers for use in a low volume DNA adenylating reaction, and DNA ligation buffers for use in low volume DNA adaptor ligation reactions with adaptors. Also disclosed are customized reagent plates and kits containing one or more of these low volume buffers for use in low volume DNA blunting, phosphorylating, adenylating, and ligation reactions. Methods of using the high concentration reagents (low volume buffers) and the customized reagent plates for preparing DNA sequencing libraries in low volume reactions are also disclosed.