发明申请
WO2017218677A2 IDENTIFICATION AND MEDICAL APPLICATIONS OF ANTI-CITRULLINATED-PROTEIN ANTIBODIES IN RHEUMATOID ARTHRITIS
审中-公开
基本信息:
- 专利标题： IDENTIFICATION AND MEDICAL APPLICATIONS OF ANTI-CITRULLINATED-PROTEIN ANTIBODIES IN RHEUMATOID ARTHRITIS
- 专利标题（中）：抗幽门螺杆菌性关节炎中抗滑石蛋白抗体的鉴定及医学应用
- 申请号：PCT/US2017/037492 申请日：2017-06-14
- 公开(公告)号：WO2017218677A2 公开(公告)日：2017-12-21
- 发明人： LABAER, Joshua , QIU, Ji , KARTHIKEYAN, Kailash , BUCKNER, Jane , NEPOM, Gerold
- 申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY , BENAROYA RESEARCH INSTITUTE AT VIRGINIA MASON
- 申请人地址： 1475 North Scottsdale Road, Suite 200 Scottsdale, AZ 85257 US
- 专利权人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY,BENAROYA RESEARCH INSTITUTE AT VIRGINIA MASON
- 当前专利权人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY,BENAROYA RESEARCH INSTITUTE AT VIRGINIA MASON
- 当前专利权人地址： 1475 North Scottsdale Road, Suite 200 Scottsdale, AZ 85257 US
- 代理机构： MILCZAREK-DESAI, Gavin, J.
- 优先权： US62/350,064 20160614
- 主分类号： G01N33/53
- IPC分类号： G01N33/53 ; C07K19/00
摘要：
Compositions and methods for detection of anti-citrullinated protein antibodies (ACPAs) in rheumatoid arthritis (RA) patients. Patient samples known or suspected of containing ACPAs were probed against citrullinated proteins, and antibody responses to 190 citrullinated proteins in 20 RA patients were investigated. Unique antibody reactivity patterns in both clinical anti-cyclic citrullinated peptide assay positive (CCP+) and negative (CCP-) RA patients were observed. At individual antigen levels, six novel antibody/antigen complexes were discovered and validated against specific citrullinated antigens (Myelin Basic Protein (MBP), osteopontin (SPP1), flap endonuclease (FENl), insulin like growth factor binding protein 6 (IGFBP6), insulin like growth factor I (IGFl) and stanniocalcin-2 (STC2)) in RA patients. Identification of immune-dominant epitope(s) for citrullinated MBP was also performed. The identified biomarkers have high specificity, especially MBP.
摘要（中）：
用于检测类风湿性关节炎（RA）患者中的抗瓜氨酸化蛋白抗体（ACPA）的组合物和方法。 对已知或怀疑含有ACPA的患者样品进行了针对瓜氨酸化蛋白质的探测，并对20名RA患者中的190种瓜氨酸化蛋白质的抗体反应进行了研究。 观察到临床抗环瓜氨酸肽测定阳性（CCP +）和阴性（CCP-）RA患者中的独特抗体反应模式。 在单独的抗原水平下，发现了六种新的抗体/抗原复合物并且针对特定的瓜氨酸抗原（髓鞘碱性蛋白（MBP），骨桥蛋白（SPP1），瓣内切核酸酶（FEN1），胰岛素样生长因子结合蛋白6（IGFBP6） 如生长因子I（IGF1）和斯钙素-2（STC2））。 还进行了瓜氨酸化MBP的免疫优势表位的鉴定。 已鉴定的生物标志物具有高度特异性，特别是MBP。