发明申请
WO2017087940A1 NON-INVASIVE MONITORING CANCER USING INTEGRATED MICROFLUIDIC PROFILING OF CIRCULATING MICROVESICLES
审中-公开
基本信息:
- 专利标题： NON-INVASIVE MONITORING CANCER USING INTEGRATED MICROFLUIDIC PROFILING OF CIRCULATING MICROVESICLES
- 专利标题（中）：循环微创综合微流控技术在非侵袭性监测中的应用
- 申请号：PCT/US2016/063044 申请日：2016-11-21
- 公开(公告)号：WO2017087940A1 公开(公告)日：2017-05-26
- 发明人： ZHAO, Zheng , ZENG, Yong , HE, Mei
- 申请人： UNIVERSITY OF KANSAS , KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
- 申请人地址： 245 Strong Hall 1450 Jayhawk Boulevard Lawrence, KS 66045 US
- 专利权人： UNIVERSITY OF KANSAS,KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
- 当前专利权人： UNIVERSITY OF KANSAS,KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
- 当前专利权人地址： 245 Strong Hall 1450 Jayhawk Boulevard Lawrence, KS 66045 US
- 代理机构： LUCEK, Nathaniel, W. et al.
- 优先权： US62/258,258 20151120
- 主分类号： B01L3/00
- IPC分类号： B01L3/00 ; G01N27/00 ; G01N33/543 ; G01N33/574
摘要：
A microfluidic exosome profiling platform integrating exosome isolation and targeted proteomic analysis is disclosed. This platform is capable of quantitative exosomal biomarker profiling directly from plasma samples with markedly enhanced sensitivity and specificity. Identification of distinct subpopulation of patient-derived exosomes is demonstrated by probing surface proteins and multiparameter analyses of intravesicular biomarkers in the selected subpopulation. The expression of IGF-IR and its phosphorylation level in non-small cell lung cancer (NSCLC) patient plasma is assessed as a non-invasive alternative to the conventional biopsy and immunohistochemistry. Detection of ovarian cancer also is assessed. The microfluidic chip, which may be fabricated of a glass substrate and a layer of poly(dimethylsiloxane), includes a serpentine microchannel to mix a fluid and a microchamber for the collection and detection of exosomes.
摘要（中）：
公开了整合外来体分离和靶向蛋白质组分析的微流体外泌体分布平台。 该平台能够直接从血浆样品中定量外泌体生物标志物分析，其灵敏度和特异性显着提高。 通过探测选定亚群中的表面蛋白和囊内生物标志物的多参数分析来证明从患者衍生的外来体的不同亚群的鉴定。 评估非小细胞肺癌（NSCLC）患者血浆中IGF-1R及其磷酸化水平的表达作为常规活检和免疫组织化学的非侵入性替代。 还评估了卵巢癌的检测。 微流控芯片可由玻璃基板和聚（二甲基硅氧烷）层制成，包括蛇形微通道以混合流体和微型腔室以收集和检测外来体。
IPC结构图谱:
| B | 作业；运输 |
| --B01 | 一般的物理或化学的方法或装置 |
| ----B01L | 通用化学或物理实验室设备 |
| ------B01L3/00 | 实验室用的容器或器皿，如实验室玻璃仪器（瓶子入B65D；酶或微生物学用的设备入C12M1/00）；点滴器 |