基本信息:
- 专利标题： SYSTEM AND METHOD FOR LONGITUDINAL ANALYSIS OF PEPTIDE SYNTHESIS
- 专利标题（中）：用于肽合成的纵向分析的系统和方法
- 申请号：PCT/EP2016/075728 申请日：2016-10-26
- 公开(公告)号：WO2017072141A1 公开(公告)日：2017-05-04
- 发明人： PATEL, Jigar , SULLIVAN, Eric , BARILOVITS, Sarah , BANNEN, Ryan , TAN, John
- 申请人： F. HOFFMANN-LA ROCHE AG , ROCHE DIAGNOSTICS GMBH , ROCHE SEQUENCING SOLUTIONS, INC.
- 申请人地址： Grenzacherstrasse 124 4070 Basel CH
- 专利权人： F. HOFFMANN-LA ROCHE AG,ROCHE DIAGNOSTICS GMBH,ROCHE SEQUENCING SOLUTIONS, INC.
- 当前专利权人： F. HOFFMANN-LA ROCHE AG,ROCHE DIAGNOSTICS GMBH,ROCHE SEQUENCING SOLUTIONS, INC.
- 当前专利权人地址： Grenzacherstrasse 124 4070 Basel CH
- 代理机构： HILDEBRANDT, Martin et al.
- 优先权： US62/247,485 20151028
- 主分类号： B01J19/00
- IPC分类号： B01J19/00 ; C07K1/04 ; C07K7/06 ; C07K17/14 ; C40B30/04 ; C40B50/16 ; G01N21/64 ; G01N33/68
摘要：
The present invention provides a system and method for assessing a synthetic peptide population including interrogating a population of peptide features in the presence of a receptor having an affinity for a binder sequence. The population of peptide features is synthesized over a plurality of synthesis periods and includes a plurality of control peptide features synthesized to have an amino acid sequence including the binder sequence. The control peptide features include a first feature synthesized beginning with a first one of the synthesis periods, and a second feature synthesized beginning after the first one of the synthesis periods such that synthesis of the second control peptide feature is delayed by at least one synthesis period. The method further includes detecting a signal output characteristic of an interaction of the receptor with the control peptide features, the signal output indicative of the fidelity of synthesis of the population of peptide features.
摘要（中）：
本发明提供了用于评估合成肽群体的系统和方法，包括在存在对粘合剂序列具有亲和力的受体时询问肽特征群体。 肽特征群在多个合成期内合成并且包括合成为具有包含粘合剂序列的氨基酸序列的多个对照肽特征。 对照肽特征包括从第一个合成期开始合成的第一个特征和在第一个合成期后开始合成的第二个特征，使得第二个对照肽特征的合成延迟了至少一个合成期 。 该方法进一步包括检测受体与对照肽特征相互作用的信号输出特征，所述信号输出指示肽特征群体合成的保真度。