基本信息:
- 专利标题： COMPOSITIONS AND METHODS FOR ANTIBODY PRODUCTION
- 专利标题（中）：用于抗体生产的组合物和方法
- 申请号：PCT/US2014/068440 申请日：2014-12-03
- 公开(公告)号：WO2015085003A1 公开(公告)日：2015-06-11
- 发明人： SINGH, Rajeeva , FISHKIN, Nathan , KITCHENER, Seth , MESHULAM, Deborah
- 申请人： IMMUNOGEN, INC.
- 申请人地址： 830 Winter Street Waltham, MA 02451-1477 US
- 专利权人： IMMUNOGEN, INC.
- 当前专利权人： IMMUNOGEN, INC.
- 当前专利权人地址： 830 Winter Street Waltham, MA 02451-1477 US
- 代理机构： HUNTER-ENSOR, Melissa et al.
- 优先权： US61/911,994 20131204; US61/969,631 20140324
- 主分类号： C12P1/00
- IPC分类号： C12P1/00
摘要：
Compositions and methods for minimizing antibody disulfide bond reduction are described. In one aspect, a composition is provided for culturing mammalian host cells to express an antibody including an anti-reduction agent that minimizes reduction of a disulfide bond in the antibody or fragment thereof. In some other aspects, methods for minimizing disulfide bond reduction; increasing production of an antibody or fragment thereof with intact native disulfide bonds; increasing a ratio of non-reduced to reduced antibody or fragment thereof; producing a therapeutic antibody or fragment thereof by adding a sufficient amount of an anti-reduction agent to a cell culture media, pre-harvest cell culture fluid, or harvest cell culture fluid are described. In another aspect, minimizing disulfide bond reduction in an antibody or fragment thereof culturing the host cell in a concentration of at least about 20% O 2 is described.
摘要（中）：
描述了最小化抗体二硫键还原的组合物和方法。 一方面，提供了一种组合物，用于培养哺乳动物宿主细胞以表达包含使抗体或其片段中二硫键还原最小化的抗还原剂的抗体。 在一些其它方面，使二硫键还原最小化的方法; 增加具有完整天然二硫键的抗体或其片段的产生; 增加未还原抗体或其片段的比例; 通过向细胞培养基，收获前细胞培养液或收获细胞培养液中添加足够量的抗还原剂来制备治疗性抗体或其片段。 在另一方面，描述了以至少约20％O 2的浓度培养宿主细胞的抗体或其片段中的二硫键还原最小化。