发明申请
WO2015054507A1 NUTRITIVE POLYPEPTIDE PRODUCTION SYSTEMS, AND METHODS OF MANUFACTURE AND USE THEREOF
审中-公开
基本信息:
- 专利标题： NUTRITIVE POLYPEPTIDE PRODUCTION SYSTEMS, AND METHODS OF MANUFACTURE AND USE THEREOF
- 专利标题（中）：营养多肽生产系统及其制造方法及其应用
- 申请号：PCT/US2014/059923 申请日：2014-10-09
- 公开(公告)号：WO2015054507A1 公开(公告)日：2015-04-16
- 发明人： BASU, Subhayu , CHEN, Ying-Ja , HARVEY, Caitlym , GORA, Katherine , BERRY, David, A. , YOUNG, David
- 申请人： PRONUTRIA, INC. , BASU, Subhayu , CHEN, Ying-Ja , HARVEY, Caitlym , GORA, Katherine , BERRY, David, A. , YOUNG, David
- 申请人地址： 840 Memorial Drive, Third Floor Cambridge, MA 02139 US
- 专利权人： PRONUTRIA, INC.,BASU, Subhayu,CHEN, Ying-Ja,HARVEY, Caitlym,GORA, Katherine,BERRY, David, A.,YOUNG, David
- 当前专利权人： PRONUTRIA, INC.,BASU, Subhayu,CHEN, Ying-Ja,HARVEY, Caitlym,GORA, Katherine,BERRY, David, A.,YOUNG, David
- 当前专利权人地址： 840 Memorial Drive, Third Floor Cambridge, MA 02139 US
- 代理机构： KABLER, Kevin et al.
- 优先权： US61/889,205 20131010
- 主分类号： C12N9/62
- IPC分类号： C12N9/62 ; C12P21/02 ; C12N15/52 ; C12R1/66 ; C12N9/22 ; C12N15/10
摘要：
The application concerns reagents and methods for the production of "nutritive polypeptides" in fungi, in particular, Aspergillus, where the expression of said polypeptide is increased by co-expressing in the fungal host (i) a variant nucleic acid encoding a secreted protease (in particular pepA, pepB, pepD, pepF or pepH), (ii) a "secretory pathway polypeptide (including a chaperone, like foldase, a vesicle trafficking polypeptide, a glycosylation pathway polypeptide or a protein degradation pathway) and/or, (ii) a nucleic acid encoding a Cas9 endonuclease plus a nucleic acid encoding a guide RNA (gRNA). The application is also directed to said nutritive proteins, nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, vectors for expressing the proteints, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins.
摘要（中）：
本申请涉及用于在真菌（特别是曲霉属）中生产“营养多肽”的试剂和方法，其中所述多肽的表达通过在真菌宿主中共表达而增加（i）编码分泌蛋白酶的变体核酸（ 特别是pepA，pepB，pepD，pepF或pepH），（ii）“分泌途径多肽（包括分子伴侣，如折叠酶，囊泡运输多肽，糖基化途径多肽或蛋白质降解途径）和/或（ii ）编码Cas9核酸内切酶加编码引导RNA（gRNA）的核酸的核酸，还涉及所述营养蛋白，编码蛋白质的核酸，制备蛋白质的重组微生物，用于表达蛋白质的载体，方法 使用重组微生物制备蛋白质，包含蛋白质的组合物和使用蛋白质的方法。