发明申请
WO2014116754A1 MATERIALS AND METHODS FOR CHARACTERIZING AND USING KASIII FOR PRODUCTION OF BI-FUNCTIONAL FATTY ACIDS
审中-公开
基本信息:
- 专利标题： MATERIALS AND METHODS FOR CHARACTERIZING AND USING KASIII FOR PRODUCTION OF BI-FUNCTIONAL FATTY ACIDS
- 专利标题（中）：表征和使用KASIII用于生产双功能脂肪酸的材料和方法
- 申请号：PCT/US2014/012616 申请日：2014-01-22
- 公开(公告)号：WO2014116754A1 公开(公告)日：2014-07-31
- 发明人： GARG, Shivani , JIN, Huanan , YANDEAU-NELSON, Marna , NIKOLAU, Basil J.
- 申请人： IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
- 申请人地址： 310 Lab of Mechanics Ames, Iowa 5011-2131 US
- 专利权人： IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
- 当前专利权人： IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
- 当前专利权人地址： 310 Lab of Mechanics Ames, Iowa 5011-2131 US
- 代理机构： LARCHER, Carol
- 优先权： US61/755,946 20130123
- 主分类号： C12N1/20
- IPC分类号： C12N1/20 ; C12P7/64 ; C12P7/44
摘要：
A mutant Bacillus subtilis , which does not express a functional KASIIIA and/or KASIIIB, and method of making; a mutant Rhodospirillum rubrum , which does not express a functional PhaC1, PhaC2, and/or PhaC3, and method of making; method of characterizing substrate specificity of KASIII; method of making mutant KASIII with altered substrate specificity and/or altered level of activity and nucleic acid, vector, host cell/organism, and mutant KASIII; an in vitro , high-throughput spectrophotometric method of assaying KASIII activity; and materials and methods for using KASIII for production of bi-functional fatty acids and the materials so produced.
摘要（中）：
不表达功能性KASIIIA和/或KASIIIB的突变型枯草芽孢杆菌及其制备方法; 不表达功能性PhaC1，PhaC2和/或PhaC3的突变体Rhodospirillum rubrum及其制备方法; 表征KASIII底物特异性的方法; 具有改变的底物特异性和/或改变的活性水平和核酸，载体，宿主细胞/生物体和突变型KASIII的突变KASIII的方法; 体外，高通量分光光度法测定KASIII活性; 以及使用KASIII生产双功能脂肪酸的材料和方法以及如此生产的材料。