基本信息:
- 专利标题: A THERMOSTABLE SUCROSE AND SUCROSE-6'-PHOSPHATE PHOSPHORYLASE
- 专利标题(中):可食用的蔗糖和蔗糖-6'-磷酸酶
- 申请号:PCT/EP2013/071587 申请日:2013-10-16
- 公开(公告)号:WO2014060452A1 公开(公告)日:2014-04-24
- 发明人: DE WINTER, Karel , SOETAERT, Wim , DESMET, Tom , AERTS, Dirk , VERHAEGHE, Tom
- 申请人: UNIVERSITEIT GENT
- 申请人地址: Sint-Pietersnieuwstraat 25 B-9000 Gent BE
- 专利权人: UNIVERSITEIT GENT
- 当前专利权人: UNIVERSITEIT GENT
- 当前专利权人地址: Sint-Pietersnieuwstraat 25 B-9000 Gent BE
- 代理机构: JACOBS, Philippe
- 优先权: EP12188966.1 20121018; EP13159683.5 20130318
- 主分类号: C12N9/10
- IPC分类号: C12N9/10 ; C12N5/00 ; C07K14/00
摘要:
The present invention relates to the identification, recombinant expression and characterization of a sucrose and sucrose-6'-phosphate phosphorylase of the thermophilic bacterium Thermoanaerobacterium thermosaccharolyticum. The enzyme has an optimal temperature of about 55°C and has a high specificity for its substrates. More importantly, the sucrose and sucrose-6'-phosphate phosphorylase of the present invention has a half-life of at least 60 hours at the industrially relevant temperature of about 60°C. This enzyme is thus -for example-useful for the industrial production of D-fructose,D-fructose-6-phosphate,alpha-D-glucose -1-phosphate or alpha-D-glucosides.
摘要(中):
本发明涉及嗜热细菌热酵母杆菌的蔗糖和蔗糖-6'-磷酸磷酸化酶的鉴定,重组表达和表征。 该酶具有约55℃的最佳温度,对其底物具有高特异性。 更重要的是,本发明的蔗糖和蔗糖-6'-磷酸化磷酸酶在工业上约60℃的温度下具有至少60小时的半衰期。 因此,该酶例如可用于D-果糖,D-果糖-6-磷酸,α-D-葡萄糖-1-磷酸或α-D-葡糖苷的工业生产。