基本信息:
- 专利标题: ENZYME CONSTRUCT
- 申请号:PCT/GB2013/051928 申请日:2013-07-18
- 公开(公告)号:WO2014013262A1 公开(公告)日:2014-01-23
- 发明人: HERON, Andrew , CLARKE, James , MOYSEY, Ruth , WALLACE, Jayne , BRUCE, Mark , JAYASINGHE, Lakmal , CAPROTTI, Domenico , SOROS, Szabolcs , MCNEILL, Luke , MISCA, Mihaela
- 申请人: OXFORD NANOPORE TECHNOLOGIES LIMITED
- 申请人地址: Edmund Cartwright House 4 Robert Robinson Avenue Oxford Science Park Oxford Oxfordshire OX4 4GA GB
- 专利权人: OXFORD NANOPORE TECHNOLOGIES LIMITED
- 当前专利权人: OXFORD NANOPORE TECHNOLOGIES LIMITED
- 当前专利权人地址: Edmund Cartwright House 4 Robert Robinson Avenue Oxford Science Park Oxford Oxfordshire OX4 4GA GB
- 代理机构: CHAPMAN, Lee Phillip
- 优先权: US61/673,446 20120719; US61/774,694 20130308
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
摘要:
The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
摘要(中):
本发明涉及使用包含解旋酶和另外的多核苷酸结合部分的构建体的方法。 解旋酶连接到多核苷酸结合部分,并且构建体具有控制多核苷酸运动的能力。 该构建体可用于控制多核苷酸的移动,并且特别可用于测序多核苷酸。