基本信息:
- 专利标题： NESTED HYBRIDIZATION PNA PROBE SYSTEM HAVING PARALLEL BINDING STRUCTURE
- 申请号：PCT/KR2012005458 申请日：2012-07-10
- 公开(公告)号：WO2013009070A2 公开(公告)日：2013-01-17
- 发明人： KIM SUNG-KEE , PARK CHWANG SIEK , JOE GOON HO , PARK HEEKYUNG , CHOI JI-AH , CHOI SUNG ROK , KIM SU NAM , KIM YONGTAE , KIM SE RYUN
- 申请人： PANAGENE INC , KIM SUNG-KEE , PARK CHWANG SIEK , JOE GOON HO , PARK HEEKYUNG , CHOI JI-AH , CHOI SUNG ROK , KIM SU NAM , KIM YONGTAE , KIM SE RYUN
- 专利权人： PANAGENE INC,KIM SUNG-KEE,PARK CHWANG SIEK,JOE GOON HO,PARK HEEKYUNG,CHOI JI-AH,CHOI SUNG ROK,KIM SU NAM,KIM YONGTAE,KIM SE RYUN
- 当前专利权人： PANAGENE INC,KIM SUNG-KEE,PARK CHWANG SIEK,JOE GOON HO,PARK HEEKYUNG,CHOI JI-AH,CHOI SUNG ROK,KIM SU NAM,KIM YONGTAE,KIM SE RYUN
- 优先权： KR20110068667 2011-07-12
- 主分类号： C12N15/11
- IPC分类号： C12N15/11 ; C12Q1/68
摘要：
The present invention relates to two peptide nucleic acid (PNA) probe systems having a parallel binding structure for real-time amplification of nucleic acid, which can be nested-hybridized with a target nucleotide, a composition comprising the probe system, and a method for detection using same. By using the composition comprising the PNA probe system, according to the present invention, the existence of a nucleotide to be detected can be confirmed, the amount of the nucleotide can be decided at a fixed quantity, single nucleotide polymorphism (SNP), which is difficult to detect with existing technique, can be detected with high sensitivity, and simultaneous multiplexed detection of nucleotides of at least two types existing in a sample can be possible.