基本信息:
- 专利标题： REVERSE TRANSCRIPTASE HAVING IMPROVED THERMOSTABILITY
- 申请号：PCT/KR2012000894 申请日：2012-02-07
- 公开(公告)号：WO2012108672A9 公开(公告)日：2012-11-01
- 发明人： PARK HAN OH , YANG SUNG JUN , JOO SUNG MO , HWANG BYOUNG OH
- 申请人： BIONEER CORP , PARK HAN OH , YANG SUNG JUN , JOO SUNG MO , HWANG BYOUNG OH
- 专利权人： BIONEER CORP,PARK HAN OH,YANG SUNG JUN,JOO SUNG MO,HWANG BYOUNG OH
- 当前专利权人： BIONEER CORP,PARK HAN OH,YANG SUNG JUN,JOO SUNG MO,HWANG BYOUNG OH
- 优先权： KR20110011639 2011-02-09
- 主分类号： C12N9/12
- IPC分类号： C12N9/12 ; C12N15/54 ; C12N15/63
摘要：
The present invention relates to a reverse transcriptase having improved thermostability, more precisely a mutant reverse transcriptase with improved thermostability by substitution of one or more amino acids selected from the group consisting of the 63rd glutamine (Q63), the 264th lysine (K264), the 295th lysine (K295), the 306th threonine (T306), the 346th glutamic acid (E346), the 408th proline (P408), the 438th histidine (H438), and the 454th asparagin (N454) of the amino acid sequence of M-MLV originated reverse transcriptase represented by SEQ. ID. NO: 1 with other amino acids. The mutant reverse transcriptase of the present invention demonstrates excellent thermostability, compared with the wild type reverse transcriptase. Therefore, it is advantageous to obtain the target cDNA with stable reverse transcription activity even in the presence of RNA that can form the stable secondary structure at a high temperature.
公开/授权文献:
- WO2012108672A3 REVERSE TRANSCRIPTASE HAVING IMPROVED THERMOSTABILITY 公开/授权日：2012-12-20