基本信息:
- 专利标题: PROCESSES OF PURIFYING STEVIOL GLYCOSIDES REB C
- 专利标题(中):纯化葡萄糖苷的方法
- 申请号:PCT/CA2012/000048 申请日:2012-01-16
- 公开(公告)号:WO2012094752A1 公开(公告)日:2012-07-19
- 发明人: ZHANG, Yong Luke , LI, Cunbiao Kevin
- 申请人: GLG LIFE TECH CORPORATION , ZHANG, Yong Luke , LI, Cunbiao Kevin
- 申请人地址: Suite 2168, 1050 West Pender Street Vancouver, BC V6E 3S7 CA
- 专利权人: GLG LIFE TECH CORPORATION,ZHANG, Yong Luke,LI, Cunbiao Kevin
- 当前专利权人: GLG LIFE TECH CORPORATION,ZHANG, Yong Luke,LI, Cunbiao Kevin
- 当前专利权人地址: Suite 2168, 1050 West Pender Street Vancouver, BC V6E 3S7 CA
- 代理机构: BEN-OLIEL, Susan Margaret
- 优先权: CN201110007515.0 20110114
- 主分类号: C07H15/24
- IPC分类号: C07H15/24 ; A23L1/236 ; C07H13/08
摘要:
A process for producing a natural sweetening enhancer composition comprising at least an Rebaudioside C (RC) extract, said process comprises the steps of preparing a saccharide mother liquor comprising an RC mass content of at least 15%; preparing feed liquid from about 8-25 mg/L of the mother liquor; flowing feed liquid through a porous adsorption column, having a pore size of between about 0.001 to 0,.2 micron, and at a flow rate of between 25 to 35 L/m2h and at a pH of between 6 to 8; eluting RC extract with alcohol, said RC extract having a mass concentration of at least 10%; fractionally collecting eluate based on chromatographic critical point for RC extract; concentrating the RC extract and drying the extract so formed. Another process for preparing a crude RC extract which comprises the steps of preparing a saccharide mother liquor into a feedstock solution with a mass concentration of about 0.5-1%; passing the solution through an ultrafiltration membrane device at a flow rate of 25-35 L/m2h, with a molecular weight cut-off at about 5500-6500 DA and at a pH of between about 6.5-7.5, collecting and then concentrating the RC filtrate from about 55°C to 65°C„ drying solid and liquid fractions obtained from the concentrate separately to provide a crude RC extract. Extracts obtained from processes above are further refined by crystallization from water-alcohols (e.g. ethanol and methanol)-acetone mixed solvents to provide a crystalline solid comprising high content of RC.
摘要(中):
一种生产包含至少一种雷鲍迪甙C(RC)提取物的天然甜味增强剂组合物的方法,所述方法包括以下步骤:制备包含至少15%的RC质量含量的糖母液; 从约8-25mg / L的母液制备饲料液; 流动的进料液体通过多孔吸附塔,孔径为约0.001至0.2微米,流速为25至35升/平方米,pH为6至8; 用乙醇洗脱RC提取物,所述RC提取物的质量浓度至少为10%; 基于RC提取物的色谱临界点分馏收集洗脱液; 浓缩RC提取物并干燥所形成的提取物。 制备粗制RC提取物的另一种方法,其包括以下步骤:将糖母液制备成质量浓度为约0.5-1%的原料溶液; 将溶液通过超滤膜装置,流速为25-35L / m2h,分子量截止值在约5500-6500DA,pH值在约6.5-7.5之间,收集然后浓缩RC 从约55℃至65℃的滤液“,分离从浓缩物中得到的固体和液体组分,以提供粗制的RC提取物。通过从水醇(例如乙醇和甲醇) - 丙酮的结晶进一步精制得到的提取物 混合溶剂以提供包含高含量RC的结晶固体。
IPC结构图谱:
C | 化学;冶金 |
--C07 | 有机化学 |
----C07H | 糖类;及其衍生物;核苷;核苷酸;核酸 |
------C07H15/00 | 含有直接连在糖化物基团的杂原子上的烃基或取代烃基的化合物 |
--------C07H15/20 | .碳环类 |
----------C07H15/24 | ..含有3个或更多的环的稠环系 |